Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
October-2016 Volume 38 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2016 Volume 38 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Mitochondrial DNA depletion causes decreased ROS production and resistance to apoptosis

  • Authors:
    • Hulin Chen
    • Junling Wang
    • Zhongrong Liu
    • Huilan Yang
    • Yingjie Zhu
    • Minling Zhao
    • Yan Liu
    • Miaomiao Yan
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, Guangzhou General Hospital of Guangzhou Military Command (Liuhuaqiao Hospital), Guangzhou, Guangdong 510010, P.R. China, Gynecologic Department of Guangzhou Hospital of Integrated Traditional and West Medicine, Guangzhou, Guangdong 510800, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1039-1046
    |
    Published online on: August 3, 2016
       https://doi.org/10.3892/ijmm.2016.2697
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Mitochondrial DNA (mtDNA) depletion occurs frequently in many diseases including cancer. The present study was designed in order to examine the hypothesis that mtDNA‑depleted cells are resistant to apoptosis and to explore the possible mechanisms responsible for this effect. Parental human osteosarcoma 143B cells and mtDNA‑deficient (Rho˚ or ρ˚) 206 cells (derived from 143B cells) were exposed to different doses of solar-simulated ultraviolet (UV) radiation. The effects of solar irradiation on cell morphology were observed under both light and fluorescence microscopes. Furthermore, apoptosis, mitochondrial membrane potential (MMP) disruption and reactive oxygen species (ROS) production were detected and measured by flow cytometry. In both cell lines, apoptosis and ROS production were clearly increased, whereas MMP was slightly decreased. However, apoptosis and ROS production were reduced in the Rho˚206 cells compared with the 143B cells. We also performed western blot analysis and demonstrated the increased release of cytosolic Cyt c from mitochondria in the 143B cells compared with that in the Rho˚206 cells. Thus, we concluded that Rho˚206 cells exhibit more resistance to solar‑simulated UV radiation‑induced apoptosis at certain doses than 143B cells and this is possibly due to decreased ROS production.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Orrenius S, Gogvadze V and Zhivotovsky B: Mitochondrial oxidative stress: implications for cell death. Annu Rev Pharmacol Toxicol. 47:143–183. 2007. View Article : Google Scholar

2 

King MP and Attardi G: Human cells lacking mtDNA: repopulation with exogenous mitochondria by complementation. Science. 246:500–503. 1989. View Article : Google Scholar : PubMed/NCBI

3 

Chandel NS and Schumacker PT: Cells depleted of mitochondrial DNA (ϱ°) yield insight into physiological mechanisms. FEBS Lett. 454:173–176. 1999. View Article : Google Scholar : PubMed/NCBI

4 

Higuchi M, Aggarwal BB and Yeh ET: Activation of CPP32-like protease in tumor necrosis factor-induced apoptosis is dependent on mitochondrial function. J Clin Invest. 99:1751–1758. 1997. View Article : Google Scholar : PubMed/NCBI

5 

Amuthan G, Biswas G, Zhang SY, Klein-Szanto A, Vijayasarathy C and Avadhani NG: Mitochondria-to-nucleus stress signaling induces phenotypic changes, tumor progression and cell invasion. EMBO J. 20:1910–1920. 2001. View Article : Google Scholar : PubMed/NCBI

6 

Biswas G, Anandatheerthavarada HK, Zaidi M and Avadhani NG: Mitochondria to nucleus stress signaling: a distinctive mechanism of NFkappaB/Rel activation through calcineurin-mediated inactivation of IkappaBbeta. J Cell Biol. 161:507–519. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Friedberg E, Walker G and Siede W: DNA repair and mutagenesis. ASM Press; Washington, DC: 1995

8 

Shi Y, Wang CH and Gong XG: Apoptosis-inducing effects of two anthraquinones from Hedyotis diffusa WILLD. Biol Pharm Bull. 31:1075–1078. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Lu M, Gong X, Lu Y, Guo J, Wang C and Pan Y: Molecular cloning and functional characterization of a cell-permeable superoxide dismutase targeted to lung adenocarcinoma cells. Inhibition cell proliferation through the Akt/p27kip1 pathway. J Biol Chem. 281:13620–13627. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Li J, Xu Z, Tan M, Su W and Gong XG: 3-(4-(Benzo[d) thiazol-2-yl)-1-phenyl-1H-pyrazol-3-yl) phenyl acetate induced Hep G2 cell apoptosis through a ROS-mediated pathway. Chem Biol Interact. 183:341–348. 2010. View Article : Google Scholar

11 

Gong K, Chen C, Zhan Y, Chen Y, Huang Z and Li W: Autophagy-related gene 7 (ATG7) and reactive oxygen species/extracellular signal-regulated kinase regulate tetrandrine-induced autophagy in human hepatocellular carcinoma. J Biol Chem. 287:35576–35588. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Lewis LD, Hamzeh FM and Lietman PS: Ultrastructural changes associated with reduced mitochondrial DNA and impaired mitochondrial function in the presence of 2′3′-dideoxycytidine. Antimicrob Agents Chemother. 36:2061–2065. 1992. View Article : Google Scholar : PubMed/NCBI

13 

Amuthan G, Biswas G, Ananadatheerthavarada HK, Vijayasarathy C, Shephard HM and Avadhani NG: Mitochondrial stress-induced calcium signaling, phenotypic changes and invasive behavior in human lung carcinoma A549 cells. Oncogene. 21:7839–7849. 2002. View Article : Google Scholar : PubMed/NCBI

14 

Xiong Y, Liu X, Lee CP, Chua BH and Ho YS: Attenuation of doxorubicin-induced contractile and mitochondrial dysfunction in mouse heart by cellular glutathione peroxidase. Free Radic Biol Med. 41:46–55. 2006. View Article : Google Scholar : PubMed/NCBI

15 

López E, Arce C, Oset-Gasque MJ, Cañadas S and González MP: Cadmium induces reactive oxygen species generation and lipid peroxidation in cortical neurons in culture. Free Radic Biol Med. 40:940–951. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES and Wang X: Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell. 91:479–489. 1997. View Article : Google Scholar : PubMed/NCBI

17 

Hojo M, Morimoto T, Maluccio M, Asano T, Morimoto K, Lagman M, Shimbo T and Suthanthiran M: Cyclosporine induces cancer progression by a cell-autonomous mechanism. Nature. 397:530–534. 1999. View Article : Google Scholar : PubMed/NCBI

18 

Kehrer JP: Free radicals as mediators of tissue injury and disease. Crit Rev Toxicol. 23:21–48. 1993. View Article : Google Scholar : PubMed/NCBI

19 

Lenaz G: Role of mitochondria in oxidative stress and ageing. Biochim Biophys Acta. 1366:53–67. 1998. View Article : Google Scholar : PubMed/NCBI

20 

Pinz KG and Bogenhagen DF: Efficient repair of abasic sites in DNA by mitochondrial enzymes. Mol Cell Biol. 18:1257–1265. 1998. View Article : Google Scholar : PubMed/NCBI

21 

Allen JA and Coombs MM: Covalent binding of polycyclic aromatic compounds to mitochondrial and nuclear DNA. Nature. 287:244–245. 1980. View Article : Google Scholar : PubMed/NCBI

22 

Backer JM and Weinstein IB: Mitochondrial DNA is a major cellular target for a dihydrodiol-epoxide derivative of benzo[a) pyrene. Science. 209:297–299. 1980. View Article : Google Scholar : PubMed/NCBI

23 

Niranjan BG, Bhat NK and Avadhani NG: Preferential attack of mitochondrial DNA by aflatoxin B1 during hepatocarcinogenesis. Science. 215:73–75. 1982. View Article : Google Scholar : PubMed/NCBI

24 

Horton TM, Petros JA, Heddi A, Shoffner J, Kaufman AE, Graham SD Jr, Gramlich T and Wallace DC: Novel mitochondrial DNA deletion found in a renal cell carcinoma. Genes Chromosomes Cancer. 15:95–101. 1996. View Article : Google Scholar : PubMed/NCBI

25 

Polyak K, Li Y, Zhu H, Lengauer C, Willson JK, Markowitz SD, Trush MA, Kinzler KW and Vogelstein B: Somatic mutations of the mitochondrial genome in human colorectal tumours. Nat Genet. 20:291–293. 1998. View Article : Google Scholar : PubMed/NCBI

26 

Fliss MS, Usadel H, Caballero OL, Wu L, Buta MR, Eleff SM, Jen J and Sidransky D: Facile detection of mitochondrial DNA mutations in tumors and bodily fluids. Science. 287:2017–2019. 2000. View Article : Google Scholar : PubMed/NCBI

27 

Yeh JJ, Lunetta KL, van Orsouw NJ, Moore FD Jr, Mutter GL, Vijg J, Dahia PL and Eng C: Somatic mitochondrial DNA (mtDNA) mutations in papillary thyroid carcinomas and differential mtDNA sequence variants in cases with thyroid tumours. Oncogene. 19:2060–2066. 2000. View Article : Google Scholar : PubMed/NCBI

28 

Loeb LA: A mutator phenotype in cancer. Cancer Res. 61:3230–3239. 2001.PubMed/NCBI

29 

Rasmussen AK, Chatterjee A, Rasmussen LJ and Singh KK: Mitochondria-mediated nuclear mutator phenotype in Saccharomyces cerevisiae. Nucleic Acids Res. 31:3909–3917. 2003. View Article : Google Scholar : PubMed/NCBI

30 

Masaki H, Atsumi T and Sakurai H: Detection of hydrogen peroxide and hydroxyl radicals in murine skin fibroblasts under UVB irradiation. Biochem Biophys Res Commun. 206:474–479. 1995. View Article : Google Scholar : PubMed/NCBI

31 

Jurkiewicz BA and Buettner GR: EPR detection of free radicals in UV-irradiated skin: mouse versus human. Photochem Photobiol. 64:918–922. 1996. View Article : Google Scholar : PubMed/NCBI

32 

Barber LA, Spandau DF, Rathman SC, Murphy RC, Johnson CA, Kelley SW, Hurwitz SA and Travers JB: Expression of the platelet-activating factor receptor results in enhanced ultraviolet B radiation-induced apoptosis in a human epidermal cell line. J Biol Chem. 273:18891–18897. 1998. View Article : Google Scholar : PubMed/NCBI

33 

Brenneisen P, Wenk J, Klotz LO, Wlaschek M, Briviba K, Krieg T and Scharffetter-Kochanek K: Central role of ferrous/ferric iron in the ultraviolet B irradiation-mediated signaling pathway leading to increased interstitial collagenase (matrix-degrading metalloprotease (MMP)-1) and stromelysin-1 (MMP-3) mRNA levels in cultured human dermal fibroblasts. Biol Chem. 273:5279–5287. 1998. View Article : Google Scholar

34 

Yasui H and Sakurai H: Chemiluminescent detection and imaging of reactive oxygen species in live mouse skin exposed to UVA. Biochem Biophys Res Commun. 269:131–136. 2000. View Article : Google Scholar : PubMed/NCBI

35 

Kang S, Chung JH, Lee JH, Fisher GJ, Wan YS, Duell EA and Voorhees JJ: Topical N-acetyl cysteine and genistein prevent ultraviolet-light-induced signaling that leads to photoaging in human skin in vivo. J Invest Dermatol. 120:835–841. 2003. View Article : Google Scholar : PubMed/NCBI

36 

Yamamoto Y: Role of active oxygen species and antioxidants in photoaging. J Dermatol Sci. 27:1–4. 2001. View Article : Google Scholar

37 

Chandra D, Liu JW and Tang DG: Early mitochondrial activation and cytochrome c up-regulation during apoptosis. J Biol Chem. 277:50842–50854. 2002. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Chen H, Wang J, Liu Z, Yang H, Zhu Y, Zhao M, Liu Y and Yan M: Mitochondrial DNA depletion causes decreased ROS production and resistance to apoptosis. Int J Mol Med 38: 1039-1046, 2016.
APA
Chen, H., Wang, J., Liu, Z., Yang, H., Zhu, Y., Zhao, M. ... Yan, M. (2016). Mitochondrial DNA depletion causes decreased ROS production and resistance to apoptosis. International Journal of Molecular Medicine, 38, 1039-1046. https://doi.org/10.3892/ijmm.2016.2697
MLA
Chen, H., Wang, J., Liu, Z., Yang, H., Zhu, Y., Zhao, M., Liu, Y., Yan, M."Mitochondrial DNA depletion causes decreased ROS production and resistance to apoptosis". International Journal of Molecular Medicine 38.4 (2016): 1039-1046.
Chicago
Chen, H., Wang, J., Liu, Z., Yang, H., Zhu, Y., Zhao, M., Liu, Y., Yan, M."Mitochondrial DNA depletion causes decreased ROS production and resistance to apoptosis". International Journal of Molecular Medicine 38, no. 4 (2016): 1039-1046. https://doi.org/10.3892/ijmm.2016.2697
Copy and paste a formatted citation
x
Spandidos Publications style
Chen H, Wang J, Liu Z, Yang H, Zhu Y, Zhao M, Liu Y and Yan M: Mitochondrial DNA depletion causes decreased ROS production and resistance to apoptosis. Int J Mol Med 38: 1039-1046, 2016.
APA
Chen, H., Wang, J., Liu, Z., Yang, H., Zhu, Y., Zhao, M. ... Yan, M. (2016). Mitochondrial DNA depletion causes decreased ROS production and resistance to apoptosis. International Journal of Molecular Medicine, 38, 1039-1046. https://doi.org/10.3892/ijmm.2016.2697
MLA
Chen, H., Wang, J., Liu, Z., Yang, H., Zhu, Y., Zhao, M., Liu, Y., Yan, M."Mitochondrial DNA depletion causes decreased ROS production and resistance to apoptosis". International Journal of Molecular Medicine 38.4 (2016): 1039-1046.
Chicago
Chen, H., Wang, J., Liu, Z., Yang, H., Zhu, Y., Zhao, M., Liu, Y., Yan, M."Mitochondrial DNA depletion causes decreased ROS production and resistance to apoptosis". International Journal of Molecular Medicine 38, no. 4 (2016): 1039-1046. https://doi.org/10.3892/ijmm.2016.2697
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team