p43 induces IP-10 expression through the JAK-STAT signaling pathway in HMEC-1 cells

  • Authors:
    • Wei Wang
    • Junjie Tan
    • Yuhua Xing
    • Naipeng Kan
    • Jingyi Ling
    • Guifu Dong
    • Gang Liu
    • Huipeng Chen
  • View Affiliations

  • Published online on: August 19, 2016     https://doi.org/10.3892/ijmm.2016.2710
  • Pages: 1217-1224
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Abstract

p43 is a cofactor of aminoacyl-tRNA synthetase in mammals that effectively inhibits angiogenesis. However, the role of p43 in angiogenesis remains unclear. In the present study, we examined the effects of p43 on angiogenesis using human microvascular endothelial cells-1 (HMEC-1) cells as a model. Our microarray data showed that p43 regulated a number of cytokines, and the majoity of these are involved in the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) pathway. IP-10 was previously shown to inhibit angiogenesis and suppress tumor growth via the JAK-STAT signaling pathway in vitro and in vivo. Our results showed that p43 induces both the mRNA and protein expression of IP-10. Furthermore, we demonstrated that p43 exerted an effect on the JAK-STAT signaling pathway by regulating key factors of the pathway. Using a JAK inhibitor, AG490, we studied the effect of p43 on HMEC-1 cells by blocking the JAK-STAT pathway. We found that AG490 inhibited the induction of IP-10 expression by p43, and suppressed the inhibitory effect of p43 on tubule formation and cell migration in HMEC-1 cells. We concluded that p43 inhibits tubule formation and cell migration by inducing IP-10 through the JAK-STAT signaling pathway, and blocking the JAK-STAT pathway with AG490 diminishes the inhibitory effects of p43 on angiogenesis.
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October-2016
Volume 38 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Wang W, Tan J, Xing Y, Kan N, Ling J, Dong G, Liu G and Chen H: p43 induces IP-10 expression through the JAK-STAT signaling pathway in HMEC-1 cells. Int J Mol Med 38: 1217-1224, 2016.
APA
Wang, W., Tan, J., Xing, Y., Kan, N., Ling, J., Dong, G. ... Chen, H. (2016). p43 induces IP-10 expression through the JAK-STAT signaling pathway in HMEC-1 cells. International Journal of Molecular Medicine, 38, 1217-1224. https://doi.org/10.3892/ijmm.2016.2710
MLA
Wang, W., Tan, J., Xing, Y., Kan, N., Ling, J., Dong, G., Liu, G., Chen, H."p43 induces IP-10 expression through the JAK-STAT signaling pathway in HMEC-1 cells". International Journal of Molecular Medicine 38.4 (2016): 1217-1224.
Chicago
Wang, W., Tan, J., Xing, Y., Kan, N., Ling, J., Dong, G., Liu, G., Chen, H."p43 induces IP-10 expression through the JAK-STAT signaling pathway in HMEC-1 cells". International Journal of Molecular Medicine 38, no. 4 (2016): 1217-1224. https://doi.org/10.3892/ijmm.2016.2710