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Anti-atherogenic effect of Humulus japonicus in apolipoprotein E-deficient mice

  • Authors:
    • Haian Lim
    • Jung-Ran Noh
    • Yong-Hoon Kim
    • Jung Hwan Hwang
    • Kyoung-Shim Kim
    • Dong-Hee Choi
    • Min-Jeong Go
    • Sang-Seop Han
    • Won-Keun Oh
    • Chul-Ho Lee
  • View Affiliations / Copyright

    Affiliations: Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea, Department of Toxicology Evaluation, Konyang University, Daejeon 35365, Republic of Korea, Korea Bioactive Natural Material Bank, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul 08826, Republic of Korea
    Copyright: © Lim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1101-1110
    |
    Published online on: September 1, 2016
       https://doi.org/10.3892/ijmm.2016.2727
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Abstract

Humulus japonicus (HJ) is used as a traditional medicine in Korea owing to its multiple properties including anti-mycobacterial, antioxidant and antihypertensive effects. The present study aimed to examine the anti‑inflammatory and anti-atherogenic effects of a methanol extract of HJ. In lipopolysaccharide-stimulated RAW 264.7 cells, HJ significantly suppressed the mRNA expression and secretion of pro-inflammatory cytokines [tumor necrosis factor-α, interleukin (IL)-1β and IL-6)], and the release of inflammatory mediators such as nitrite and prostaglandin E2, together with a concomitant decrease in the mRNA levels of inducible nitric oxide synthase and cyclooxygenase-2. To examine whether HJ is capable of inhibiting experimental atherogenesis in an animal model, we randomly divided apolipoprotein E-deficient (apoE-/-) mice into three groups: mice fed an atherogenic diet plus vehicle (0.5% carboxymethyl cellulose) as the control vehicle group, and mice fed an atherogenic diet plus either 100 (HJ100) or 500 mg/kg (HJ500) of HJ as the experimental groups. After 12 weeks of HJ administration, lipid accumulation and the formation of atherosclerotic lesions in the aorta (en face) and the aortic sinus markedly decreased in the HJ500 group compared with the corresponding values in the vehicle control group. Moreover, monocyte and macrophage infiltration in the aortic sinus was markedly reduced in the HJ500 group. Reverse transcription-quantitative polymerase chain reaction analysis of the whole aorta showed that the mRNA levels of intercellular adhesion molecule-1, monocyte chemoattractant protein-1, CD68 and IL-18 were significantly decreased in the HJ500 group. Collectively, these findings suggest that HJ may suppress atherosclerosis by inhibiting lipid accumulation and the expression of pro-atherogenic factors, and it may be effective at preventing the development of atherosclerosis.
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Copy and paste a formatted citation
Spandidos Publications style
Lim H, Noh J, Kim Y, Hwang JH, Kim K, Choi D, Go M, Han S, Oh W, Lee C, Lee C, et al: Anti-atherogenic effect of Humulus japonicus in apolipoprotein E-deficient mice. Int J Mol Med 38: 1101-1110, 2016.
APA
Lim, H., Noh, J., Kim, Y., Hwang, J.H., Kim, K., Choi, D. ... Lee, C. (2016). Anti-atherogenic effect of Humulus japonicus in apolipoprotein E-deficient mice. International Journal of Molecular Medicine, 38, 1101-1110. https://doi.org/10.3892/ijmm.2016.2727
MLA
Lim, H., Noh, J., Kim, Y., Hwang, J. H., Kim, K., Choi, D., Go, M., Han, S., Oh, W., Lee, C."Anti-atherogenic effect of Humulus japonicus in apolipoprotein E-deficient mice". International Journal of Molecular Medicine 38.4 (2016): 1101-1110.
Chicago
Lim, H., Noh, J., Kim, Y., Hwang, J. H., Kim, K., Choi, D., Go, M., Han, S., Oh, W., Lee, C."Anti-atherogenic effect of Humulus japonicus in apolipoprotein E-deficient mice". International Journal of Molecular Medicine 38, no. 4 (2016): 1101-1110. https://doi.org/10.3892/ijmm.2016.2727
Copy and paste a formatted citation
x
Spandidos Publications style
Lim H, Noh J, Kim Y, Hwang JH, Kim K, Choi D, Go M, Han S, Oh W, Lee C, Lee C, et al: Anti-atherogenic effect of Humulus japonicus in apolipoprotein E-deficient mice. Int J Mol Med 38: 1101-1110, 2016.
APA
Lim, H., Noh, J., Kim, Y., Hwang, J.H., Kim, K., Choi, D. ... Lee, C. (2016). Anti-atherogenic effect of Humulus japonicus in apolipoprotein E-deficient mice. International Journal of Molecular Medicine, 38, 1101-1110. https://doi.org/10.3892/ijmm.2016.2727
MLA
Lim, H., Noh, J., Kim, Y., Hwang, J. H., Kim, K., Choi, D., Go, M., Han, S., Oh, W., Lee, C."Anti-atherogenic effect of Humulus japonicus in apolipoprotein E-deficient mice". International Journal of Molecular Medicine 38.4 (2016): 1101-1110.
Chicago
Lim, H., Noh, J., Kim, Y., Hwang, J. H., Kim, K., Choi, D., Go, M., Han, S., Oh, W., Lee, C."Anti-atherogenic effect of Humulus japonicus in apolipoprotein E-deficient mice". International Journal of Molecular Medicine 38, no. 4 (2016): 1101-1110. https://doi.org/10.3892/ijmm.2016.2727
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