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Article

3'-Daidzein sulfonate sodium inhibits neuronal apoptosis induced by cerebral ischemia-reperfusion

  • Authors:
    • Ruizhen Liu
    • Xinming Zhong
    • Jing Zeng
    • Zhihua Huang
    • Xiao Li
    • Hai Xiao
    • Qin Chen
    • Dongliang Li
  • View Affiliations / Copyright

    Affiliations: Gannan Medical College, Ganzhou, Jiangxi 341000, P.R. China, Key Laboratory of Cerebrovascular Diseases of Jiangxi Province, Ganzhou, Jiangxi 341000, P.R. China
  • Pages: 1021-1028
    |
    Published online on: March 10, 2017
       https://doi.org/10.3892/ijmm.2017.2915
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Abstract

This study aimed to observe the effects of 3'-daidzein sulfonate sodium (DSS) on ischemia-reperfusion-induced brain injury and to analyze the mechanisms responsible for neuronal apoptosis. Focal ischemias were induced in male Sprague-Dawley rats using middle cerebral artery occlusion. The rats were divided into 5 groups based on sham surgery or real occlusion, and treatment with different doses of DSS (0.5, 1.0 and 2.0 mg/kg) or normal saline (model group), injected preoperatively into the rats with cerebral occlusion. After 2 h of ischemia and 24 h of reperfusion, neurological deficit scores were evaluated using the Longa grade point standard. The infarct volume was measured using a triphenyl tetrazolium chloride staining technique. Blood-brain barrier (BBB) permeability was measured using the Evans blue (EB) content of brain tissues, while electron microscopy was used to observe ultrastructural changes. The expression levels of Bcl-2, Bax and caspase-3 were detected by an immunohistochemical method and western blot analysis. The neurological deficit in rats pre-treated with DSS at all doses decreased significantly (P<0.05) in comparison with the model group, as did the cerebral infarct volume ratios. The brain EB content was significantly reduced by the injection of DSS. The ultrastructural integrity of the rat BBB was significantly preserved in the DSS-treated groups in comparison with the model group. This was concomitant with the reduced swelling of astrocytes and pericytes in the BBB. The immunohistochemistry results revealed that DSS significantly enhanced the expression of Bcl-2, and inhibited the expression of Bax and caspase-3 in the brain in comparison to the model group. The number of apoptotic cells in the groups treated with DSS was reduced in comparison with similar areas in the model group. These findings suggest that DSS within a dosage range of 0.5-2.0 mg/kg provides significant protection from injury to the BBB induced by cerebral ischemia-reperfusion, as it exerts a neuroprotective effect by inhibiting apoptosis.
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View References

1 

Ministry of Health of the People's Republic of China: Summary of Chinese health statistics. Beijing: People's Medical Publishing House; 2012

2 

Sauser K, Burke JF, Reeves MJ, Barsan WG and Levine DA: A systematic review and critical appraisal of quality measures for the emergency care of acute ischemic stroke. Ann Emerg Med. 64:235–244. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Fang YL, Luo YM and Zhao YM: Protective effect and the related mechanisms of rhubarb on ischemic cerebrovascular disease. ShouDu Yikedaxue Xuebao. 36:718–722. 2015.

4 

Dong GX and Feng YP: Effects of NBP on ATPase and antioxidant enzymes activities and lipid peroxidation in transient focal cerebral ischemic rats. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 24:93–97. 2002.

5 

Liu R, Gao WJ, Qian T and Wang L: Astragalus injection inhibits expression of Apaf-1 in rat hippocampus after cerebral ischemia and reperfusion. Zhongguo Bingli Shengli Zazhi. 29:872–877. 2013.

6 

Qu HY and Yuan J: Effects of Ginsenoside Rg1 on nNOS and iNOS expressions in rat brain tissue after cerebral ischemia reperfusion. Tianjin Yiyao. 42:889–892. 2014.

7 

Li H, Deng CQ, Chen BY, Chen RF, Zhang SP and Liang Y: Effects of Panax Notoginseng saponins on expression of caspase after focal cerebral ischemia-reperfusion in rats. Chin Pharmacol Bull. 22:189–193. 2006.

8 

Li CY and Li WH: Research progress of the mechanisms of cerebral ischemia reperfusion injury. Zhonghua Xiandai Zhongxiyi Zazhi. 3:1744–1746. 2005.

9 

Kuschinsky W and Gillardon F: Apoptosis and cerebral ischemia. Cerebrovasc Dis. 10:165–169. 2000. View Article : Google Scholar : PubMed/NCBI

10 

Uchino H, Morota S, Hirabayashi G, Ushijima K, Kakinuma T, Ishii N, Shibasaki F and Kuroda Y: Molecular mechanism of ischemic brain injuries and perspectives of drug therapies for neuroprotection. Masui. 56:248–270. 2007.In Japanese. PubMed/NCBI

11 

Zhuo AS, Chen AJ and Hua WJ: Effects of cerebral ischemia and reperfusion injury on apoptosis of cell and expression of apoptosis gene in the different tissues of rats. Zhongguo Linchuang Kangfu. 6:1263–1264. 2002.

12 

Xue Q, Zou YA, Zhao AM and He XF: Effects of Kangnaoye 1 on cerebral ischemia-reperfusion half silent zone apoptosis and Bcl-2/Bax ratio in rats. Zhongguo Quanke Yixue. 13:498–501. 2010.

13 

Khalil H, Peltzer N, Walicki J, Yang JY, Dubuis G, Gardiol N, Held W, Bigliardi P, Marsland B, Liaudet L and Widmann C: Caspase-3 protects stressed organs against cell death. Mol Cell Biol. 32:4523–4533. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Yu L, Miao H, Hou Y, Zhang B and Guo L: Neuroprotective effect of A20 on TNF-induced postischemic apoptosis. Neurochem Res. 31:21–32. 2006.PubMed/NCBI

15 

Wang QH, Li DL and Huang ZH: The anti-oxidation effects of daidzin on isolated myocardial ischemia/reperfusion injury. J Gannan Med Univ. 31:189–191. 2011.

16 

Qi JP, Wu AP, Wang DS, Wang LF, Li SX and Xu FL: Correlation between neuronal injury and Caspase-3 after focal ischemia in human hippocampus. Chin Med J (Engl). 117:1507–1512. 2004.

17 

Ye HY, Chen QY, Qiu F, Huang ZH, Huang YP, Xiao H and Zeng J: Effect of daidzein on atrial electrophysiological characteristic in guinea pig. Zhong Yao Cai. 29:312–313. 2006.

18 

Ye HY, Hu ZP, Zhou L, Huang ZH and Zeng J: Protective effect of daidzein on myocardial ischemia injury in rats. Pharmacol Clin Chin Mater Med. 27:221–223. 2006.

19 

Woodman OL and Boujaoude M: Chronic treatment of male rats with daidzein and 17 beta-oestradiol induces the contribution of EDHF to endothelium-dependent relaxation. Br J Pharmacol. 141:322–328. 2004. View Article : Google Scholar

20 

Nevala R, Paukku K, Korpela R and Vapaatalo H: Calcium-sensitive potassium channel inhibitors antagonize genistein- and daidzein-induced arterial relaxation in vitro. Life Sci. 69:1407–1417. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Huang YS, Zeng J, Huang YP, Qiu F, Ye HY and Wang SR: Antagonistic effect of 3′-daidzein sulfonate sodium on prostatic hyperplasia in mice. Zhonghua Nan Ke Xue. 13:387–390. 2007.In Chinese. PubMed/NCBI

22 

Li CT, Wang YL, Wu GT, Cheng XL, She YL, Huang Y and Chen YF: Effect of Puerarin on Caspase-3 and Bcl-2 expression of hippocampal CA1 neurons in ovariectomized rats. Zhongguo Zhongyiyao Xinxi Zazhi. 21:40–46. 2014.

23 

Longa EZ, Weinstein PR, Carlson S and Cummins R: Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke. 20:84–91. 1989. View Article : Google Scholar : PubMed/NCBI

24 

Huang HF, Guo F, Cao YZ, Shi W and Xia Q: Neuroprotection by manganese superoxide dismutase (MnSOD) mimics: antioxidant effect and oxidative stress regulation in acute experimental stroke. CNS Neurosci Ther. 18:811–818. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Homi HM, Sheng H, Arepally GM, Mackensen GB and Grocott HP: Aprotinin improves functional outcome but not cerebral infarct size in an experimental model of stroke during cardiopulmonary bypass. Anesth Analg. 111:38–45. 2010.PubMed/NCBI

26 

Liu J and Wang LN: Gamma aminobutyric acid (GABA) receptor agonists for acute stroke. Cochrane Database Syst Rev. CD0096222014.PubMed/NCBI

27 

Lin Z, Zhu D, Yan Y and Yu B: Herbal formula FBD extracts prevented brain injury and inflammation induced by cerebral ischemia-reperfusion. J Ethnopharmacol. 118:140–147. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Chan SJ, Wong WS, Wong PT and Bian JS: Neuroprotective effects of andrographolide in a rat model of permanent cerebral ischaemia. Br J Pharmacol. 161:668–679. 2010. View Article : Google Scholar : PubMed/NCBI

29 

Sun K, Fan J and Han J: Ameliorating effects of traditional Chinese medicine preparation, Chinese materia medica and active compounds on ischemia/reperfusion-induced cerebral microcirculatory disturbances and neuron damage. Acta Pharm Sin B. 5:8–24. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Meng QH: Study on acute toxicity of 3′-daidzein sulfonate sodium in mice. Zhongyiyao Daobao. 19:78–79. 2013.

31 

Wang JZ and Yin CH: Pathophysiology. Beijing: People's Medical Publishing House; 2013

32 

He G, Siddik ZH, Huang Z, Wang R, Koomen J, Kobayashi R, Khokhar AR and Kuang J: Induction of p21 by p53 following DNA damage inhibits both Cdk4 and Cdk2 activities. Oncogene. 24:2929–2943. 2005. View Article : Google Scholar : PubMed/NCBI

33 

Goloudina AR, Mazur SJ, Appella E, Garrido C and Demidov ON: Wip1 sensitizes p53-negative tumors to apoptosis by regulating the Bax/Bcl-xL ratio. Cell Cycle. 11:1883–1887. 2012. View Article : Google Scholar : PubMed/NCBI

34 

Wu C, Fujihara H, Yao J, Qi S, Li H, Shimoji K and Baba H: Different expression patterns of Bcl-2, Bcl-xl, and Bax proteins after sublethal forebrain ischemia in C57Black/Crj6 mouse striatum. Stroke. 34:1803–1808. 2003. View Article : Google Scholar : PubMed/NCBI

35 

Inohara N, Gourley TS, Carrio R, Muñiz M, Merino J, Garcia I, Koseki T, Hu Y, Chen S and Núñez G: Diva, a Bcl-2 homologue that binds directly to Apaf-1 and induces BH3-independent cell death. J Biol Chem. 273:32479–32486. 1998. View Article : Google Scholar : PubMed/NCBI

36 

Jin HM: Pathophysiology. People's Medical Publishing House; Beijing: pp. 178–180. 2000

37 

Behrends M, Martinez-Palli G, Niemann CU, Cohen S, Ramachandran R and Hirose R: Acute hyperglycemia worsens hepatic ischemia/reperfusion injury in rats. J Gastrointest Surg. 14:528–535. 2010. View Article : Google Scholar :

38 

Harrison DC, Davis RP, Bond BC, Campbell CA, James MF, Parsons AA and Philpott KL: Caspase mRNA expression in a rat model of focal cerebral ischemia. Brain Res Mol Brain Res. 89:133–146. 2001. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu R, Zhong X, Zeng J, Huang Z, Li X, Xiao H, Chen Q and Li D: 3'-Daidzein sulfonate sodium inhibits neuronal apoptosis induced by cerebral ischemia-reperfusion. Int J Mol Med 39: 1021-1028, 2017.
APA
Liu, R., Zhong, X., Zeng, J., Huang, Z., Li, X., Xiao, H. ... Li, D. (2017). 3'-Daidzein sulfonate sodium inhibits neuronal apoptosis induced by cerebral ischemia-reperfusion. International Journal of Molecular Medicine, 39, 1021-1028. https://doi.org/10.3892/ijmm.2017.2915
MLA
Liu, R., Zhong, X., Zeng, J., Huang, Z., Li, X., Xiao, H., Chen, Q., Li, D."3'-Daidzein sulfonate sodium inhibits neuronal apoptosis induced by cerebral ischemia-reperfusion". International Journal of Molecular Medicine 39.4 (2017): 1021-1028.
Chicago
Liu, R., Zhong, X., Zeng, J., Huang, Z., Li, X., Xiao, H., Chen, Q., Li, D."3'-Daidzein sulfonate sodium inhibits neuronal apoptosis induced by cerebral ischemia-reperfusion". International Journal of Molecular Medicine 39, no. 4 (2017): 1021-1028. https://doi.org/10.3892/ijmm.2017.2915
Copy and paste a formatted citation
x
Spandidos Publications style
Liu R, Zhong X, Zeng J, Huang Z, Li X, Xiao H, Chen Q and Li D: 3'-Daidzein sulfonate sodium inhibits neuronal apoptosis induced by cerebral ischemia-reperfusion. Int J Mol Med 39: 1021-1028, 2017.
APA
Liu, R., Zhong, X., Zeng, J., Huang, Z., Li, X., Xiao, H. ... Li, D. (2017). 3'-Daidzein sulfonate sodium inhibits neuronal apoptosis induced by cerebral ischemia-reperfusion. International Journal of Molecular Medicine, 39, 1021-1028. https://doi.org/10.3892/ijmm.2017.2915
MLA
Liu, R., Zhong, X., Zeng, J., Huang, Z., Li, X., Xiao, H., Chen, Q., Li, D."3'-Daidzein sulfonate sodium inhibits neuronal apoptosis induced by cerebral ischemia-reperfusion". International Journal of Molecular Medicine 39.4 (2017): 1021-1028.
Chicago
Liu, R., Zhong, X., Zeng, J., Huang, Z., Li, X., Xiao, H., Chen, Q., Li, D."3'-Daidzein sulfonate sodium inhibits neuronal apoptosis induced by cerebral ischemia-reperfusion". International Journal of Molecular Medicine 39, no. 4 (2017): 1021-1028. https://doi.org/10.3892/ijmm.2017.2915
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