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Assessment of the antioxidant activity of an olive oil total polyphenolic fraction and hydroxytyrosol from a Greek Olea europea variety in endothelial cells and myoblasts

  • Authors:
    • Paraskevi Kouka
    • Alexandros Priftis
    • Dimitrios Stagos
    • Apostolis Angelis
    • Panagiotis Stathopoulos
    • Nikos Xinos
    • Alexios‑Léandros Skaltsounis
    • Charalampos Mamoulakis
    • Aristides M. Tsatsakis
    • Demetrios A. Spandidos
    • Demetrios Kouretas
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry and Biotechnology, University of Thessaly, 41500 Larissa, Greece, Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, University of Athens, 15771 Athens, Greece, PharmaGnose S.A., 34100 Eyboia, Greece, Department of Urology, University General Hospital of Heraklion, University of Crete Medical School, 71003 Heraklion, Greece, Laboratory of Toxicology, Faculty of Medicine, University of Crete, 71003 Heraklion, Greece, Laboratory of Clinical Virology, Medical School, University of Crete, 71003 Heraklion, Greece
    Copyright: © Kouka et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 703-712
    |
    Published online on: July 20, 2017
       https://doi.org/10.3892/ijmm.2017.3078
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Abstract

Olive oil (OO) constitutes the basis of the Mediterranean diet, and it seems that its biophenols, such as hydroxytyrosol (HT) may scavenge free radicals, attracting distinct attention due to their beneficial effects in many pathological conditions, such as cancer. To the best of our knowedge, this is the first study in which the functional properties of an OO total polyphenolic fraction (TPF) and pure HT were examined in order to determine their antioxidant effects at a cellular level in endothelial cells and myoblasts. The test compounds were isolated using a green gradient‑elution centrifugal partition chromatography‑based method that allows the isolation of large volumes of OO in a continuous extraction procedure and with extremely low solvent consumption. For the isolation of HT, a combination of two chromatographic techniques was used, which is effective for the recovery of pure compounds from complex natural extracts. Moreover, TPF and HT exhibited potent free radical scavenging activity in vitro. The cells were treated with non‑cytotoxic concentrations and their redox status [in terms of glutathione (GSH) and reactive oxygen species (ROS) levels] was assessed. TPF extract was less cytotoxic than HT, and the observed differences between the two cell lines used suggest a tissue‑specific activity. Finally, flow cytometric analysis revealed that both TPF and HT improved the redox status by increasing the levels of GSH, one of the most important antioxidant molecules, in both endothelial cells and myoblasts, while the ROS levels were not significantly affected.
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Copy and paste a formatted citation
Spandidos Publications style
Kouka P, Priftis A, Stagos D, Angelis A, Stathopoulos P, Xinos N, Skaltsounis AL, Mamoulakis C, Tsatsakis AM, Spandidos DA, Spandidos DA, et al: Assessment of the antioxidant activity of an olive oil total polyphenolic fraction and hydroxytyrosol from a Greek Olea europea variety in endothelial cells and myoblasts. Int J Mol Med 40: 703-712, 2017.
APA
Kouka, P., Priftis, A., Stagos, D., Angelis, A., Stathopoulos, P., Xinos, N. ... Kouretas, D. (2017). Assessment of the antioxidant activity of an olive oil total polyphenolic fraction and hydroxytyrosol from a Greek Olea europea variety in endothelial cells and myoblasts. International Journal of Molecular Medicine, 40, 703-712. https://doi.org/10.3892/ijmm.2017.3078
MLA
Kouka, P., Priftis, A., Stagos, D., Angelis, A., Stathopoulos, P., Xinos, N., Skaltsounis, A., Mamoulakis, C., Tsatsakis, A. M., Spandidos, D. A., Kouretas, D."Assessment of the antioxidant activity of an olive oil total polyphenolic fraction and hydroxytyrosol from a Greek Olea europea variety in endothelial cells and myoblasts". International Journal of Molecular Medicine 40.3 (2017): 703-712.
Chicago
Kouka, P., Priftis, A., Stagos, D., Angelis, A., Stathopoulos, P., Xinos, N., Skaltsounis, A., Mamoulakis, C., Tsatsakis, A. M., Spandidos, D. A., Kouretas, D."Assessment of the antioxidant activity of an olive oil total polyphenolic fraction and hydroxytyrosol from a Greek Olea europea variety in endothelial cells and myoblasts". International Journal of Molecular Medicine 40, no. 3 (2017): 703-712. https://doi.org/10.3892/ijmm.2017.3078
Copy and paste a formatted citation
x
Spandidos Publications style
Kouka P, Priftis A, Stagos D, Angelis A, Stathopoulos P, Xinos N, Skaltsounis AL, Mamoulakis C, Tsatsakis AM, Spandidos DA, Spandidos DA, et al: Assessment of the antioxidant activity of an olive oil total polyphenolic fraction and hydroxytyrosol from a Greek Olea europea variety in endothelial cells and myoblasts. Int J Mol Med 40: 703-712, 2017.
APA
Kouka, P., Priftis, A., Stagos, D., Angelis, A., Stathopoulos, P., Xinos, N. ... Kouretas, D. (2017). Assessment of the antioxidant activity of an olive oil total polyphenolic fraction and hydroxytyrosol from a Greek Olea europea variety in endothelial cells and myoblasts. International Journal of Molecular Medicine, 40, 703-712. https://doi.org/10.3892/ijmm.2017.3078
MLA
Kouka, P., Priftis, A., Stagos, D., Angelis, A., Stathopoulos, P., Xinos, N., Skaltsounis, A., Mamoulakis, C., Tsatsakis, A. M., Spandidos, D. A., Kouretas, D."Assessment of the antioxidant activity of an olive oil total polyphenolic fraction and hydroxytyrosol from a Greek Olea europea variety in endothelial cells and myoblasts". International Journal of Molecular Medicine 40.3 (2017): 703-712.
Chicago
Kouka, P., Priftis, A., Stagos, D., Angelis, A., Stathopoulos, P., Xinos, N., Skaltsounis, A., Mamoulakis, C., Tsatsakis, A. M., Spandidos, D. A., Kouretas, D."Assessment of the antioxidant activity of an olive oil total polyphenolic fraction and hydroxytyrosol from a Greek Olea europea variety in endothelial cells and myoblasts". International Journal of Molecular Medicine 40, no. 3 (2017): 703-712. https://doi.org/10.3892/ijmm.2017.3078
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