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Article Open Access

Identification of CALM as the potential serum biomarker for predicting the recurrence of nasopharyngeal carcinoma using a mass spectrometry-based comparative proteomic approach

  • Authors:
    • Huiling Meng
    • Xiaodong Zhu
    • Ling Li
    • Zhongguo Liang
    • Xiaoyu Li
    • Xinbin Pan
    • Fanyan Zeng
    • Song Qu
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Oncology, Affiliated Cancer Hospital of Guangxi Medical University, Cancer Institute of Guangxi Zhuang Autonomous Region, Nanning, Guangxi 530021, P.R. China
    Copyright: © Meng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1152-1164
    |
    Published online on: August 9, 2017
       https://doi.org/10.3892/ijmm.2017.3094
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Abstract

To date, there are no serum biomarkers available for the prediction of recurrent nasopharyngeal carcinoma (rNPC). The diagnosis of rNPC mostly depends on imaging and biopsy of diseased tissue; however, both of these methods work mostly if the target tumor is at an advanced stage. Therefore, the identificaqtion of recurrent biomarkers is urgently required. In the present study, we used tandem mass tag (TMT) labeling and high performance liquid chromatography (HPLC) fractionation followed by liquid chromatography-tandem mass spectrometry (LC‑MS/MS) to identify differentially expressed proteins. Serum was collected from 40 patients with NPC [recurrence (n=20) and no recurrence (n=20)]. Compared to non‑recurrent NPC (nrNPC), we found 59 proteins to be significantly dysregulated in rNPC; most of these have been previously reported to play a role in carcinogenesis. The dysregulation of calmodulin (CALM) was confirmed in 74 new patients [recurrence (n=32) and no recurrence (n=42)] by ELISA. Moreover, we performed a preliminary pathway analysis which revealed that oxidative phosphorylation was altered in the patients with rNPC compared to those with nrNPC. Taken together, these data identify a potential diagnostic biomarker for rNPC and elucidate the potential molecular mechanisms that are dysregulated and contribute to the pathogenesis of rNPC.
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Copy and paste a formatted citation
Spandidos Publications style
Meng H, Zhu X, Li L, Liang Z, Li X, Pan X, Zeng F and Qu S: Identification of CALM as the potential serum biomarker for predicting the recurrence of nasopharyngeal carcinoma using a mass spectrometry-based comparative proteomic approach. Int J Mol Med 40: 1152-1164, 2017.
APA
Meng, H., Zhu, X., Li, L., Liang, Z., Li, X., Pan, X. ... Qu, S. (2017). Identification of CALM as the potential serum biomarker for predicting the recurrence of nasopharyngeal carcinoma using a mass spectrometry-based comparative proteomic approach. International Journal of Molecular Medicine, 40, 1152-1164. https://doi.org/10.3892/ijmm.2017.3094
MLA
Meng, H., Zhu, X., Li, L., Liang, Z., Li, X., Pan, X., Zeng, F., Qu, S."Identification of CALM as the potential serum biomarker for predicting the recurrence of nasopharyngeal carcinoma using a mass spectrometry-based comparative proteomic approach". International Journal of Molecular Medicine 40.4 (2017): 1152-1164.
Chicago
Meng, H., Zhu, X., Li, L., Liang, Z., Li, X., Pan, X., Zeng, F., Qu, S."Identification of CALM as the potential serum biomarker for predicting the recurrence of nasopharyngeal carcinoma using a mass spectrometry-based comparative proteomic approach". International Journal of Molecular Medicine 40, no. 4 (2017): 1152-1164. https://doi.org/10.3892/ijmm.2017.3094
Copy and paste a formatted citation
x
Spandidos Publications style
Meng H, Zhu X, Li L, Liang Z, Li X, Pan X, Zeng F and Qu S: Identification of CALM as the potential serum biomarker for predicting the recurrence of nasopharyngeal carcinoma using a mass spectrometry-based comparative proteomic approach. Int J Mol Med 40: 1152-1164, 2017.
APA
Meng, H., Zhu, X., Li, L., Liang, Z., Li, X., Pan, X. ... Qu, S. (2017). Identification of CALM as the potential serum biomarker for predicting the recurrence of nasopharyngeal carcinoma using a mass spectrometry-based comparative proteomic approach. International Journal of Molecular Medicine, 40, 1152-1164. https://doi.org/10.3892/ijmm.2017.3094
MLA
Meng, H., Zhu, X., Li, L., Liang, Z., Li, X., Pan, X., Zeng, F., Qu, S."Identification of CALM as the potential serum biomarker for predicting the recurrence of nasopharyngeal carcinoma using a mass spectrometry-based comparative proteomic approach". International Journal of Molecular Medicine 40.4 (2017): 1152-1164.
Chicago
Meng, H., Zhu, X., Li, L., Liang, Z., Li, X., Pan, X., Zeng, F., Qu, S."Identification of CALM as the potential serum biomarker for predicting the recurrence of nasopharyngeal carcinoma using a mass spectrometry-based comparative proteomic approach". International Journal of Molecular Medicine 40, no. 4 (2017): 1152-1164. https://doi.org/10.3892/ijmm.2017.3094
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