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Article

Isoastragaloside I inhibits NF-κB activation and inflammatory responses in BV-2 microglial cells stimulated with lipopolysaccharide

  • Authors:
    • Hongshuai Liu
    • Fei Huang
    • Hui Wu
    • Beibei Zhang
    • Hailian Shi
    • Xiaojun Wu
    • Zhibi Hu
  • View Affiliations / Copyright

    Affiliations: Shanghai Key Laboratory of Compound Chinese Medicines, The Ministry of Education (MOE) Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, P.R. China
  • Pages: 1270-1276
    |
    Published online on: August 30, 2017
       https://doi.org/10.3892/ijmm.2017.3114
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Abstract

The excessive activation of microglia in many neurodegenerative diseases is detrimental to neuronal survival. Isoastragaloside I (ISO I) is a natural saponin molecule found within the roots of Astragalus membranaceus, a famous traditional Chinese medicine. In the present study, the anti‑inflammatory effects and the mechanisms of action of ISO I on activated BV-2 cells stimulated with lipopolysaccharide (LPS) were investigated. ISO I dose‑dependently inhibited the excessive release of nitric oxide (NO) and tumor necrosis factor (TNF)-α in the LPS-stimulated BV-2 cells. Moreover, it decreased the production of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2), and mitigated the gene expression of interleukin (IL)-1β, TNF-α and iNOS induced by LPS. Further experiments revealed that ISO I decreased the phosphorylation levels of nuclear factor-κB (NF-κB), and suppressed its nuclear translocation and transactivation activity. In addition, it inhibited the activation of signaling pathway molecules, such as PI3K, Akt and mitogen-activated protein kinases (MAPKs). Taken together, our findings suggest that ISO I prevents LPS-induced microglial activation probably by inhibiting the activation of the NF-κB via PI3K/Akt and MAPK signaling pathways, indicating its therapeutic potential for neurological diseases relevant to neuroinflammation.
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Copy and paste a formatted citation
Spandidos Publications style
Liu H, Huang F, Wu H, Zhang B, Shi H, Wu X and Hu Z: Isoastragaloside I inhibits NF-κB activation and inflammatory responses in BV-2 microglial cells stimulated with lipopolysaccharide. Int J Mol Med 40: 1270-1276, 2017.
APA
Liu, H., Huang, F., Wu, H., Zhang, B., Shi, H., Wu, X., & Hu, Z. (2017). Isoastragaloside I inhibits NF-κB activation and inflammatory responses in BV-2 microglial cells stimulated with lipopolysaccharide. International Journal of Molecular Medicine, 40, 1270-1276. https://doi.org/10.3892/ijmm.2017.3114
MLA
Liu, H., Huang, F., Wu, H., Zhang, B., Shi, H., Wu, X., Hu, Z."Isoastragaloside I inhibits NF-κB activation and inflammatory responses in BV-2 microglial cells stimulated with lipopolysaccharide". International Journal of Molecular Medicine 40.4 (2017): 1270-1276.
Chicago
Liu, H., Huang, F., Wu, H., Zhang, B., Shi, H., Wu, X., Hu, Z."Isoastragaloside I inhibits NF-κB activation and inflammatory responses in BV-2 microglial cells stimulated with lipopolysaccharide". International Journal of Molecular Medicine 40, no. 4 (2017): 1270-1276. https://doi.org/10.3892/ijmm.2017.3114
Copy and paste a formatted citation
x
Spandidos Publications style
Liu H, Huang F, Wu H, Zhang B, Shi H, Wu X and Hu Z: Isoastragaloside I inhibits NF-κB activation and inflammatory responses in BV-2 microglial cells stimulated with lipopolysaccharide. Int J Mol Med 40: 1270-1276, 2017.
APA
Liu, H., Huang, F., Wu, H., Zhang, B., Shi, H., Wu, X., & Hu, Z. (2017). Isoastragaloside I inhibits NF-κB activation and inflammatory responses in BV-2 microglial cells stimulated with lipopolysaccharide. International Journal of Molecular Medicine, 40, 1270-1276. https://doi.org/10.3892/ijmm.2017.3114
MLA
Liu, H., Huang, F., Wu, H., Zhang, B., Shi, H., Wu, X., Hu, Z."Isoastragaloside I inhibits NF-κB activation and inflammatory responses in BV-2 microglial cells stimulated with lipopolysaccharide". International Journal of Molecular Medicine 40.4 (2017): 1270-1276.
Chicago
Liu, H., Huang, F., Wu, H., Zhang, B., Shi, H., Wu, X., Hu, Z."Isoastragaloside I inhibits NF-κB activation and inflammatory responses in BV-2 microglial cells stimulated with lipopolysaccharide". International Journal of Molecular Medicine 40, no. 4 (2017): 1270-1276. https://doi.org/10.3892/ijmm.2017.3114
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