A novel method of neural differentiation of PC12 cells by using Opti-MEM as a basic induction medium

Hu,Rendong; Cao,Qiaoyu; Sun,Zhongqing; Chen,Jinying; Zheng,Qing; Xiao,Fei

doi:10.3892/ijmm.2017.3195

A novel method of neural differentiation of PC12 cells by using Opti-MEM as a basic induction medium

Authors:
- Rendong Hu
- Qiaoyu Cao
- Zhongqing Sun
- Jinying Chen
- Qing Zheng
- Fei Xiao
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Affiliations: Department of Pharmacology, School of Medicine, Jinan University, Guangzhou, Guangdong 510632, P.R. China, College of Pharmacy, Jinan University, Guangzhou, Guangdong 510632, P.R. China, Department of Anesthesia and Intensive Care, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong 999077, SAR, P.R. China, Department of Ophthalmology, The First Clinical Medical College of Jinan University, Guangzhou, Guangdong 510632, P.R. China
Published online on: October 19, 2017 https://doi.org/10.3892/ijmm.2017.3195
Pages: 195-201
Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The PC12 cell line is a classical neuronal cell model due to its ability to acquire the sympathetic neurons features when deal with nerve growth factor (NGF). In the present study, the authors used a variety of different methods to induce PC12 cells, such as Opti-MEM medium containing different concentrations of fetal bovine serum (FBS) and horse serum compared with RPMI-1640 medium, and then observed the neurite length, differentiation, adhesion, cell proliferation and action potential, as well as the protein levels of axonal growth-associated protein 43 (GAP-43) and synaptic protein synapsin-1, among other differences. Compared with the conventional RPMI-1640 medium induction method, the new approach significantly improved the neurite length of induced cells (2.7 times longer), differentiation rate (30% increase), adhesion rate (21% increase) and expression of GAP-43 and synapsin-1 (three times), as well as reduced cell proliferation. The morphology of induced cells in Opti-MEM medium containing 0.5% FBS was more like that of neurons. Additionally, induced cells were also able to motivate the action potential after treatment for 6 days. Therefore, the research provided a novel, improved induction method of neural differentiation of PC12 cells using Opti-MEM medium containing 0.5% FBS, resulting in a better neuronal model cell line that can be widely used in neurobiology and neuropharmacology research.

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