Quercetin inhibits okadaic acid-induced tau protein hyperphosphorylation through the Ca2+‑calpain‑p25‑CDK5 pathway in HT22 cells

  • Authors:
    • Xiu‑Yin Shen
    • Tao Luo
    • Sheng Li
    • Ou‑Yang Ting
    • Feng He
    • Jie Xu
    • Hua‑Qiao Wang
  • View Affiliations

  • Published online on: November 22, 2017     https://doi.org/10.3892/ijmm.2017.3281
  • Pages: 1138-1146
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Abstract

Alzheimer's disease (AD) is a common neurodegenerative disorder characterized by aberrant tau protein hyperphosphorylation, which eventually leads to the formation of neurofibrillary tangles. Hyperphosphorylated tau protein is considered as a vital factor in the development of AD and is highly associated with cognitive impairment. Therefore, it is recognized to be a potential therapeutic target. Quercetin (QUE) is a naturally occurring flavonoid compound. In the present study, the inhibitory effect of QUE on okadaic acid (OA)-induced tau protein hyperphosphorylation in HT22 cells was explored. Western blotting results indicated that QUE significantly attenuated OA‑induced tau protein hyperphosphorylation at the Ser396, Ser199, Thr231 and Thr205 sites. Further experiments demonstrated that QUE inhibited the activity of cyclin‑dependent kinase 5 (CDK5), a key enzyme in the regulation of tau protein, and blocked the Ca2+‑calpain‑p25‑CDK5 signaling pathway. These observations indicate the ability of QUE to decrease tau protein hyperphosphorylation and thereby attenuate the associated neuropathology. In conclusion, these results support the potential of QUE as a therapeutic agent for AD and other neurodegenerative tauopathies.
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February-2018
Volume 41 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Shen XY, Luo T, Li S, Ting OY, He F, Xu J and Wang HQ: Quercetin inhibits okadaic acid-induced tau protein hyperphosphorylation through the Ca2+‑calpain‑p25‑CDK5 pathway in HT22 cells. Int J Mol Med 41: 1138-1146, 2018
APA
Shen, X., Luo, T., Li, S., Ting, O., He, F., Xu, J., & Wang, H. (2018). Quercetin inhibits okadaic acid-induced tau protein hyperphosphorylation through the Ca2+‑calpain‑p25‑CDK5 pathway in HT22 cells. International Journal of Molecular Medicine, 41, 1138-1146. https://doi.org/10.3892/ijmm.2017.3281
MLA
Shen, X., Luo, T., Li, S., Ting, O., He, F., Xu, J., Wang, H."Quercetin inhibits okadaic acid-induced tau protein hyperphosphorylation through the Ca2+‑calpain‑p25‑CDK5 pathway in HT22 cells". International Journal of Molecular Medicine 41.2 (2018): 1138-1146.
Chicago
Shen, X., Luo, T., Li, S., Ting, O., He, F., Xu, J., Wang, H."Quercetin inhibits okadaic acid-induced tau protein hyperphosphorylation through the Ca2+‑calpain‑p25‑CDK5 pathway in HT22 cells". International Journal of Molecular Medicine 41, no. 2 (2018): 1138-1146. https://doi.org/10.3892/ijmm.2017.3281