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Molecular expression of multiple Nav1.5 splice variants in the frontal lobe of the human brain

  • Authors:
    • Jun Wang
    • Shao‑Wu Ou
    • Zhi‑Yong Zhang
    • Bo Qiu
    • Yun‑Jie Wang
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 915-923
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    Published online on: November 24, 2017
       https://doi.org/10.3892/ijmm.2017.3286
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Abstract

Voltage-gated sodium channels serve an essential role in the initiation and propagation of action potentials for central neurons. Previous studies have demonstrated that two novel variants of Nav1.5, designated Nav1.5e and Nav1.5f, were expressed in the human brain cortex. To date, nine distinct sodium channel isoforms of Nav1.5 have been identified. In the present study, the expression of Nav1.5 splice variants in the frontal lobe of the human brain cortex was systematically investigated. The results demonstrated that wild Nav1.5 and its splice variants, Nav1.5c and Nav1.5e, were expressed in the frontal lobe of the human brain cortex. Nav1.5a, Nav1.5b and Nav1.5d splice variants were not detected. However, the expression level of different Nav1.5 variants was revealed to vary. The expression ratio of wild Nav1.5 vs. Nav1.5c and Nav1.5e was approximately 5:1 and 1:5, respectively. Immunochemistry results revealed that Nav1.5 immunoreactivity was predominantly in neuronal cell bodies and processes, including axons and dendrites, whereas little immunoreactivity was detected in the glial components. These results revealed that a minimum of four Nav1.5 splice variants are expressed in the frontal lobe of the human brain cortex. This indicates that the previously reported tetrodotoxin‑resistant sodium current was a compound product of different Nav1.5 variants. The present study revealed that Nav1.5 channels have a more abundant expression in the human brain than previously considered. It also provided further insight into the complexity and functional significance of Nav1.5 channels in human brain neurons.
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Copy and paste a formatted citation
Spandidos Publications style
Wang J, Ou SW, Zhang ZY, Qiu B and Wang YJ: Molecular expression of multiple Nav1.5 splice variants in the frontal lobe of the human brain. Int J Mol Med 41: 915-923, 2018.
APA
Wang, J., Ou, S., Zhang, Z., Qiu, B., & Wang, Y. (2018). Molecular expression of multiple Nav1.5 splice variants in the frontal lobe of the human brain. International Journal of Molecular Medicine, 41, 915-923. https://doi.org/10.3892/ijmm.2017.3286
MLA
Wang, J., Ou, S., Zhang, Z., Qiu, B., Wang, Y."Molecular expression of multiple Nav1.5 splice variants in the frontal lobe of the human brain". International Journal of Molecular Medicine 41.2 (2018): 915-923.
Chicago
Wang, J., Ou, S., Zhang, Z., Qiu, B., Wang, Y."Molecular expression of multiple Nav1.5 splice variants in the frontal lobe of the human brain". International Journal of Molecular Medicine 41, no. 2 (2018): 915-923. https://doi.org/10.3892/ijmm.2017.3286
Copy and paste a formatted citation
x
Spandidos Publications style
Wang J, Ou SW, Zhang ZY, Qiu B and Wang YJ: Molecular expression of multiple Nav1.5 splice variants in the frontal lobe of the human brain. Int J Mol Med 41: 915-923, 2018.
APA
Wang, J., Ou, S., Zhang, Z., Qiu, B., & Wang, Y. (2018). Molecular expression of multiple Nav1.5 splice variants in the frontal lobe of the human brain. International Journal of Molecular Medicine, 41, 915-923. https://doi.org/10.3892/ijmm.2017.3286
MLA
Wang, J., Ou, S., Zhang, Z., Qiu, B., Wang, Y."Molecular expression of multiple Nav1.5 splice variants in the frontal lobe of the human brain". International Journal of Molecular Medicine 41.2 (2018): 915-923.
Chicago
Wang, J., Ou, S., Zhang, Z., Qiu, B., Wang, Y."Molecular expression of multiple Nav1.5 splice variants in the frontal lobe of the human brain". International Journal of Molecular Medicine 41, no. 2 (2018): 915-923. https://doi.org/10.3892/ijmm.2017.3286
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