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Article Open Access

Protective effect of angiotensin-(1-7) against hyperglycaemia-induced injury in H9c2 cardiomyoblast cells via the PI3K̸Akt signaling pathway

Corrigendum in: /10.3892/ijmm.2017.3322
  • Authors:
    • Yi-Ying Yang
    • Xiu-Ting Sun
    • Zheng-Xun Li
    • Wei-Yan Chen
    • Xiang Wang
    • Mei-Ling Liang
    • Hui Shi
    • Zhi-Sheng Yang
    • Wu-Tao Zeng
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdong 510080, P.R. China, Intensive Care Unit, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510260, P.R. China, Department of Cardiology, Laiwu City People's Hospital, Laiwu, Shandong 27110, P.R. China, Department of Cardiology, Sun Yat-Sen Cardiovascular Hospital, Shenzhen, Guangdong 518020, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].
  • Pages: 1283-1292
    |
    Published online on: December 15, 2017
       https://doi.org/10.3892/ijmm.2017.3322
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Abstract

Angiotensin-(1-7) [Ang-(1-7)], a heptapeptide mainly generated from cleavage of AngⅠ and AngⅡ, possesses physiological and pharmacological properties, including anti‑inflammatory and antidiabetic properties. Activation of the phosphoinositide 3-kinase and protein kinase B (PI3K̸Akt) signaling pathway has been confirmed to participate in cardioprotection against hyperglycaemia-induced injury. The aim of the present study was to test the hypothesis that Ang-(1-7) protects H9c2 cardiomyoblast cells against high glucose (HG)-induced injury by activating the PI3K̸Akt pathway. To examine this hypothesis, H9c2 cells were treated with 35 mmol/l (mM) glucose (HG) for 24 h to establish a HG-induced cardiomyocyte injury model. The cells were co-treated with 1 µmol/l (µM) Ang-(1-7) and 35 mM glucose. The findings of the present study demonstrated that exposure of H9c2 cells to HG for 24 h markedly induced injury, as evidenced by an increase in the percentage of apoptotic cells, generation of reactive oxygen species and level of inflammatory cytokines, as well as a decline in cell viability and mitochondrial luminosity. These injuries were significantly attenuated by co-treatment of the cells with Ang-(1-7) and HG. In addition, PI3K̸Akt phosphorylation was suppressed by HG treatment, but this effect was abolished when the H9c2 cells were co-treated with Ang-(1-7) and HG. Furthermore, the cardioprotection of Ang-(1-7) against HG-induced injury in H9c2 cardiomyoblasts was highly attenuated in the presence of either D-Ala7-Ang-(1-7) (A-779, an antagonist of the Mas receptor) or LY294002 (an inhibitor of PI3K̸Akt). In conclusion, the present study provided new evidence that Ang-(1-7) protects H9c2 cardiomyoblasts against HG-induced injury by activating the PI3K̸Akt signaling pathway.
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Yang Y, Sun X, Li Z, Chen W, Wang X, Liang M, Shi H, Yang Z and Zeng W: Protective effect of angiotensin-(1-7) against hyperglycaemia-induced injury in H9c2 cardiomyoblast cells via the PI3K̸Akt signaling pathway Corrigendum in /10.3892/ijmm.2017.3322. Int J Mol Med 41: 1283-1292, 2018.
APA
Yang, Y., Sun, X., Li, Z., Chen, W., Wang, X., Liang, M. ... Zeng, W. (2018). Protective effect of angiotensin-(1-7) against hyperglycaemia-induced injury in H9c2 cardiomyoblast cells via the PI3K̸Akt signaling pathway Corrigendum in /10.3892/ijmm.2017.3322. International Journal of Molecular Medicine, 41, 1283-1292. https://doi.org/10.3892/ijmm.2017.3322
MLA
Yang, Y., Sun, X., Li, Z., Chen, W., Wang, X., Liang, M., Shi, H., Yang, Z., Zeng, W."Protective effect of angiotensin-(1-7) against hyperglycaemia-induced injury in H9c2 cardiomyoblast cells via the PI3K̸Akt signaling pathway Corrigendum in /10.3892/ijmm.2017.3322". International Journal of Molecular Medicine 41.3 (2018): 1283-1292.
Chicago
Yang, Y., Sun, X., Li, Z., Chen, W., Wang, X., Liang, M., Shi, H., Yang, Z., Zeng, W."Protective effect of angiotensin-(1-7) against hyperglycaemia-induced injury in H9c2 cardiomyoblast cells via the PI3K̸Akt signaling pathway Corrigendum in /10.3892/ijmm.2017.3322". International Journal of Molecular Medicine 41, no. 3 (2018): 1283-1292. https://doi.org/10.3892/ijmm.2017.3322
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Y, Sun X, Li Z, Chen W, Wang X, Liang M, Shi H, Yang Z and Zeng W: Protective effect of angiotensin-(1-7) against hyperglycaemia-induced injury in H9c2 cardiomyoblast cells via the PI3K̸Akt signaling pathway Corrigendum in /10.3892/ijmm.2017.3322. Int J Mol Med 41: 1283-1292, 2018.
APA
Yang, Y., Sun, X., Li, Z., Chen, W., Wang, X., Liang, M. ... Zeng, W. (2018). Protective effect of angiotensin-(1-7) against hyperglycaemia-induced injury in H9c2 cardiomyoblast cells via the PI3K̸Akt signaling pathway Corrigendum in /10.3892/ijmm.2017.3322. International Journal of Molecular Medicine, 41, 1283-1292. https://doi.org/10.3892/ijmm.2017.3322
MLA
Yang, Y., Sun, X., Li, Z., Chen, W., Wang, X., Liang, M., Shi, H., Yang, Z., Zeng, W."Protective effect of angiotensin-(1-7) against hyperglycaemia-induced injury in H9c2 cardiomyoblast cells via the PI3K̸Akt signaling pathway Corrigendum in /10.3892/ijmm.2017.3322". International Journal of Molecular Medicine 41.3 (2018): 1283-1292.
Chicago
Yang, Y., Sun, X., Li, Z., Chen, W., Wang, X., Liang, M., Shi, H., Yang, Z., Zeng, W."Protective effect of angiotensin-(1-7) against hyperglycaemia-induced injury in H9c2 cardiomyoblast cells via the PI3K̸Akt signaling pathway Corrigendum in /10.3892/ijmm.2017.3322". International Journal of Molecular Medicine 41, no. 3 (2018): 1283-1292. https://doi.org/10.3892/ijmm.2017.3322
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