Placenta‑associated serum exosomal miR‑155 derived from patients with preeclampsia inhibits eNOS expression in human umbilical vein endothelial cells

  • Authors:
    • Li Shen
    • Yujing Li
    • Ruotian Li
    • Zhenyu Diao
    • Muyi Yany
    • Mengfei Wu
    • Haixiang Sun
    • Guijun Yan
    • Yali Hu
  • View Affiliations

  • Published online on: January 8, 2018     https://doi.org/10.3892/ijmm.2018.3367
  • Pages: 1731-1739
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Abstract

Preeclampsia (PE) is considered to be initiated by abnormal placentation in early pregnancy and results in systemic endothelial cell dysfunction in the second or third trimester. MicroRNAs (miRs) expressed in the human placenta can be secreted into maternal circulation via exosomes, which are secreted extracellular vesicles that serve important roles in intercellular communication. The present study hypothesized that upregulation of placenta‑associated serum exosomal miR‑155 from patients with PE may suppress endothelial nitric oxide synthase (eNOS) expression in endothelial cells. The results demonstrated that placenta‑associated serum exosomes from patients with PE decreased nitric oxide (NO) production and eNOS expression in primary human umbilical vein endothelial cells (HUVECs). Subsequently, an upregulation of placenta‑associated serum exosomal miR‑155 was detected in patients with PE compared with in gestational age‑matched normal pregnant women. In addition, the results demonstrated that overexpression of exosomal miR‑155 from BeWo cells was internalized into HUVECs, and was able to suppress eNOS expression by targeting its 3'‑untranslated region. The results of the present study indicated that placenta‑associated serum exosomes may inhibit eNOS expression in endothelial cell during PE development in humans, and this phenomenon may be partly due to increased miR‑155 expression in placenta‑associated serum exosomes.
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March 2018
Volume 41 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Shen, L., Li, Y., Li, R., Diao, Z., Yany, M., Wu, M. ... Hu, Y. (2018). Placenta‑associated serum exosomal miR‑155 derived from patients with preeclampsia inhibits eNOS expression in human umbilical vein endothelial cells. International Journal of Molecular Medicine, 41, 1731-1739. https://doi.org/10.3892/ijmm.2018.3367
MLA
Shen, L., Li, Y., Li, R., Diao, Z., Yany, M., Wu, M., Sun, H., Yan, G., Hu, Y."Placenta‑associated serum exosomal miR‑155 derived from patients with preeclampsia inhibits eNOS expression in human umbilical vein endothelial cells". International Journal of Molecular Medicine 41.3 (2018): 1731-1739.
Chicago
Shen, L., Li, Y., Li, R., Diao, Z., Yany, M., Wu, M., Sun, H., Yan, G., Hu, Y."Placenta‑associated serum exosomal miR‑155 derived from patients with preeclampsia inhibits eNOS expression in human umbilical vein endothelial cells". International Journal of Molecular Medicine 41, no. 3 (2018): 1731-1739. https://doi.org/10.3892/ijmm.2018.3367