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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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March-2018 Volume 41 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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Article

miR‑214 reduces cisplatin resistance by targeting netrin‑1 in bladder cancer cells

  • Authors:
    • Jiao Liu
    • Jianbin Bi
    • Zeliang Li
    • Zhenhua Li
    • Xiankui Liu
    • Chuize Kong
  • View Affiliations / Copyright

    Affiliations: Department of Urology, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China
  • Pages: 1765-1773
    |
    Published online on: January 10, 2018
       https://doi.org/10.3892/ijmm.2018.3374
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Abstract

miR‑214 has been reported to be downregulated in several cancer types, such as bladder cancer. However, its involvement in apoptosis and chemoresistance has not been investigated. The present study aimed to clarify the biological function of miR‑214 and potential mechanisms in chemoresistance of bladder cancer cells. Reverse transcription‑quantitative polymerase chain reaction demonstrated that miR‑214 was downregulated in bladder cancer tissues compared with the level in normal tissues. miR‑214 was downregulated in bladder cancer cell lines compared with the level in the normal cell line SV‑HUC‑1. miR‑214 mimics were transfected into T24 and J82 cell lines to restore its expression. The results indicated that miR‑214 mimic inhibited proliferation and invasion in these cell lines. In addition, miR‑214 mimic reduced cisplatin resistance in T24 and J82 cells, indicated by the inhibition of cell viability and upregulation of cell apoptosis. Western blotting demonstrated that miR‑214 mimic was able to upregulate cleaved caspase‑3 and cleaved poly (ADP‑ribose) polymerase (PARP), while downregulate caspase‑3 and PARP expression, and AKT phosphorylation. Using prediction software, it was revealed that the netrin‑1 oncoprotein is on the target list of miR‑214. miR‑214 also downregulated netrin‑1 protein and mRNA expression levels in the T24 and J82 cell lines. Luciferase reporter assays demonstrated that netrin‑1 acted as a direct target of miR‑214. A negative correlation between netrin‑1 and miR‑214 expression in bladder cancer tissues was also observed. In addition, cisplatin treatment could induce netrin‑1 protein expression in bladder cancer cells and miR‑214 mimic partly blocked this phenomenon. Netrin‑1 plasmid transfection inhibited cisplatin‑induced apoptosis, upregulated AKT phosphorylation, and downregulated caspase‑3 and PARP cleavage. Netrin‑1 was restored in cells transfected with miR‑214 mimic using plasmid transfection. Netrin‑1 transfection restored AKT phosphorylation and blocked caspase/PARP cleavage in the T24 and J82 cell lines. In conclusion, the present study demonstrated that miR‑214 is downregulated in bladder cancer tissues and cell lines. miR‑214 reduces chemoresistance by targeting netrin‑1 in bladder cancer cell lines.
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Copy and paste a formatted citation
Spandidos Publications style
Liu J, Bi J, Li Z, Li Z, Liu X and Kong C: miR‑214 reduces cisplatin resistance by targeting netrin‑1 in bladder cancer cells. Int J Mol Med 41: 1765-1773, 2018.
APA
Liu, J., Bi, J., Li, Z., Li, Z., Liu, X., & Kong, C. (2018). miR‑214 reduces cisplatin resistance by targeting netrin‑1 in bladder cancer cells. International Journal of Molecular Medicine, 41, 1765-1773. https://doi.org/10.3892/ijmm.2018.3374
MLA
Liu, J., Bi, J., Li, Z., Li, Z., Liu, X., Kong, C."miR‑214 reduces cisplatin resistance by targeting netrin‑1 in bladder cancer cells". International Journal of Molecular Medicine 41.3 (2018): 1765-1773.
Chicago
Liu, J., Bi, J., Li, Z., Li, Z., Liu, X., Kong, C."miR‑214 reduces cisplatin resistance by targeting netrin‑1 in bladder cancer cells". International Journal of Molecular Medicine 41, no. 3 (2018): 1765-1773. https://doi.org/10.3892/ijmm.2018.3374
Copy and paste a formatted citation
x
Spandidos Publications style
Liu J, Bi J, Li Z, Li Z, Liu X and Kong C: miR‑214 reduces cisplatin resistance by targeting netrin‑1 in bladder cancer cells. Int J Mol Med 41: 1765-1773, 2018.
APA
Liu, J., Bi, J., Li, Z., Li, Z., Liu, X., & Kong, C. (2018). miR‑214 reduces cisplatin resistance by targeting netrin‑1 in bladder cancer cells. International Journal of Molecular Medicine, 41, 1765-1773. https://doi.org/10.3892/ijmm.2018.3374
MLA
Liu, J., Bi, J., Li, Z., Li, Z., Liu, X., Kong, C."miR‑214 reduces cisplatin resistance by targeting netrin‑1 in bladder cancer cells". International Journal of Molecular Medicine 41.3 (2018): 1765-1773.
Chicago
Liu, J., Bi, J., Li, Z., Li, Z., Liu, X., Kong, C."miR‑214 reduces cisplatin resistance by targeting netrin‑1 in bladder cancer cells". International Journal of Molecular Medicine 41, no. 3 (2018): 1765-1773. https://doi.org/10.3892/ijmm.2018.3374
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