Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
May-2018 Volume 41 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2018 Volume 41 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

UVB induces apoptosis via downregulation of CALML3-dependent JNK1/2 and ERK1/2 pathways in cataract

  • Authors:
    • Yin Jia
    • Qin Qin
    • Chao‑Ping Fang
    • Wei Shen
    • Ting‑Ting Sun
    • Yuan‑Lan Huang
    • Wen‑Jie Li
    • An‑Mei Deng
  • View Affiliations / Copyright

    Affiliations: Department of Laboratory Diagnosis, Changhai Hospital, The Second Military Medical University, Shanghai 200433, P.R. China, Department of Ophthalmology, Changhai Hospital, The Second Military Medical University, Shanghai 200433, P.R. China, Medical Department, The 455th Hospital of PLA, Shanghai 200052, P.R. China
  • Pages: 3041-3050
    |
    Published online on: February 9, 2018
       https://doi.org/10.3892/ijmm.2018.3478
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of the current study was to understand the mechanisms of apoptosis occurring in cultured human lens epithelial cells (HLECs) following ultraviolet B (UVB) irradiation. The investigations intended to confirm the presence of apoptosis and to reveal the roles of oxidative stress, calcium (Ca2+), c‑Jun NH2‑terminal kinase (JNK)1/2, and extracellular signal‑regulated kinase (ERK)1/2 signaling pathway in these progresses. Cell apoptosis, ROS generation and intracellular Ca2+ concentration was measured by flow cytometry. The expression of CALML3, caspase-3, Bax, Bcl-2, p-JNK1/2, JNK1/2, p-ERK1/2 and ERK1/2 was measured by RT-qPCR and western blot analysis. Annexin V‑fluorescein isothiocyanate/propidium iodide staining demonstrated that UVB irradiation increased the apoptotic rate, reactive oxygen species (ROS) production and intracellular Ca2+ concentration of HLECs in dose‑ and time‑dependent manners. Overexpression of calmodulin like 3 (CALML3) reversed the effects of UVB irradiation on apoptosis, ROS production and Ca2+ concentration of HLECs, and decreased expressions of caspase‑3 and Bax, with increased expressions of Bcl‑2. Notably, silencing of CALML3 had similar effects to UVB irradiation and inhibited the activation JNK1/2 and ERK1/2 pathways. Nimodipine, a Ca2+‑channel antagonist, significantly attenuated the damages induced by CALML3 downregulation. In conclusion, UVB irradiation induced increase in apoptosis, ROS production and Ca2+ concentration of HLECs, in part, by downregulating the expression of CALML3 and involved oxidative stress, Ca2+, JNK1/2 and ERK1/2 signaling pathways, suggesting that investigating CALML3 may useful for developing cataract treatment.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

View References

1 

Shibata S, Singh DP, Shibata N, Sasaki H and Kubo E: Hyperglycemia-and oxidative stress-induced hyperoxidation of Prdx 6 during lens epithelial cell damage and rat lens opacity in vitro and in vivo. Invest Ophthalmol Vis Sci. 55:5723. 2014.

2 

Giblin FJ, Lin L-R, Leverenz VR and Dang L: A class I (Senofilcon A) soft contact lens prevents UVB-induced ocular effects, including cataract, in the rabbit in vivo. Invest Ophthalmol Vis Sci. 52:3667–3675. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Wu Q, Guo D, Bi H, Wang D and Du Y: UVB irradiation-induced dysregulation of plasma membrane calcium ATPase1 and intracellular calcium homeostasis in human lens epithelial cells. Mol Cell Biochem. 382:263–272. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Zhang J, Yan H, Löfgren S, Tian X and Lou MF: Ultraviolet radiation-induced cataract in mice: The effect of age and the potential biochemical mechanism. Invest Ophthalmol Vis Sci. 53:7276–7285. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Kim J, Kim OS, Kim C-S, Sohn E, Jo K and Kim JS: Accumulation of argpyrimidine, a methylglyoxal-derived advanced glycation end product, increases apoptosis of lens epithelial cells both in vitro and in vivo. Exp Mol Med. 44:167–175. 2012. View Article : Google Scholar :

6 

Sinha K, Das J, Pal PB and Sil PC: Oxidative stress: The mitochondria-dependent and mitochondria-independent pathways of apoptosis. Arch Toxicol. 87:1157–1180. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Ji Y, Cai L, Zheng T, Ye H, Rong X, Rao J and Lu Y: The mechanism of UVB irradiation induced-apoptosis in cataract. Mol Cell Biochem. 401:87–95. 2015. View Article : Google Scholar

8 

Zwang Y and Yarden Y: p38 MAP kinase mediates stress-induced internalization of EGFR: Implications for cancer chemotherapy. EMBO J. 25:4195–4206. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Roduit R and Schorderet DF: MAP kinase pathways in UV-induced apoptosis of retinal pigment epithelium ARPE19 cells. Apoptosis. 13:343–353. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Chang D, Zhang X, Rong S, Sha Q, Liu P, Han T and Pan H: Serum antioxidative enzymes levels and oxidative stress products in age-related cataract patients. Oxid Med Cell Longev. 2013:5878262013. View Article : Google Scholar : PubMed/NCBI

11 

Wojcik M, Burzynska-Pedziwiatr I and Wozniak LA: A review of natural and synthetic antioxidants important for health and longevity. Curr Med Chem. 17:3262–3288. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Yonar ME: The effect of lycopene on oxytetracycline-induced oxidative stress and immunosuppression in rainbow trout (Oncorhynchus mykiss, W.). Fish Shellfish Immunol. 32:994–1001. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Dechandt CR, De Souza DL, Siqueira JT, Pereira MP, De Assis RP, Da Silva VC, De Sousa PT Jr, Brunetti IL, Andrade CM, Kawashita NH, et al: Changes in the oxidative stress biomarkers in liver of streptozotocin-diabetic rats treated with combretum lanceolatum flowers extract. Br J Pharm Res. 4:2340–2356. 2014. View Article : Google Scholar

14 

Chang D, Zhang X, Rong S, Sha Q, Liu P, Han T and Pan H: Serum antioxidative enzymes levels and oxidative stress products in age-related cataract patients. Oxid Med Cell Longev. 2013:5878262013. View Article : Google Scholar : PubMed/NCBI

15 

Strehler EE: Emanuel Strehler's work on calcium pumps and calcium signaling. World J Biol Chem. 2:67–72. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Chang KC, Lee AS, Chen WY, Lin YN, Hsu JF, Chan HC, Chang CM, Chang SS, Pan CC, Sawamura T, et al: Increased LDL electronegativity in chronic kidney disease disrupts calcium homeostasis resulting in cardiac dysfunction. J Mol Cell Cardiol. 84:36–44. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Fu S, Yang L, Li P, Hofmann O, Dicker L, Hide W, Lin X, Watkins SM, Ivanov AR and Hotamisligil GS: Aberrant lipid metabolism disrupts calcium homeostasis causing liver endoplasmic reticulum stress in obesity. Nature. 473:528–531. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Walton JR: Aluminum disruption of calcium homeostasis and signal transduction resembles change that occurs in aging and Alzheimer's disease. J Alzheimers Dis. 29:255–273. 2012.PubMed/NCBI

19 

Brooks MD, Bennett RD, Weaver AL, Sebo TJ, Eckert SE, Strehler EE and Carr AB: Human calmodulin-like protein CALML3: a novel marker for normal oral squamous mucosa that is downregulated in malignant transformation. Int J Dent. 2013:5928432013. View Article : Google Scholar : PubMed/NCBI

20 

Bennett RD, Pittelkow MR and Strehler EE: Immunolocalization of the tumor-sensitive calmodulin-like protein CALML3 in normal human skin and hyperproliferative skin disorders. PLoS One. 8:e623472013. View Article : Google Scholar : PubMed/NCBI

21 

Calenda G, Suadicani SO, Iglesias R, Spray DC, Melman A and Davies KP: Silencing MaxiK activity in corporal smooth muscle cells initiates compensatory mechanisms to maintain calcium homeostasis. J Sex Med. 8:2191–2204. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Livak and Schmittgen: Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method. Methods. 25:402–408. 2001. View Article : Google Scholar

23 

Cao G, Chen M, Song Q, Liu Y, Xie L, Han Y, Liu Z, Ji Y and Jiang Q: EGCG protects against UVB-induced apoptosis via oxidative stress and the JNK1/c-Jun pathway in ARPE19 cells. Mol Med Rep. 5:54–59. 2012.

24 

Tsukahara S, Yamamoto S, Tin-Tin-Win-Shwe, Ahmed S, Kunugita N, Arashidani K and Fujimaki H: Inhalation of low-level formaldehyde increases the Bcl-2/Bax expression ratio in the hippocampus of immunologically sensitized mice. Neuroimmunomodulation. 13:63–68. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Xu GX and Wang TT: Apoptosis of lens epithelial cells induced by cinobufagin in vitro. Int J Ophthalmol. 3:128–131. 2010.PubMed/NCBI

26 

Klein B, Myers C, Cruickshanks K, Lee K and Klein R: Inflammatory and oxidative stress markers and the 20-year cumulative incidence of age-related cataract: The beaver dam eye study. Invest Ophthalmol Vis Sci. 54:897. 2013.

27 

Berthoud VM and Beyer EC: Oxidative stress, lens gap junctions, and cataracts. Antioxid Redox Signal. 11:339–353. 2009. View Article : Google Scholar :

28 

Brennan LA and Kantorow M: Mitochondrial function and redox control in the aging eye: Role of MsrA and other repair systems in cataract and macular degenerations. Exp Eye Res. 88:195–203. 2009. View Article : Google Scholar :

29 

Scharf J and Dovrat A: Superoxide dismutase molecules in human cataractous lenses. Ophthalmic Res. 18:332–337. 1986. View Article : Google Scholar : PubMed/NCBI

30 

Wang D, Wang Z, Li Y, Chen X and Sun GY: Nimodipine inhibits N-methyl-N-nitrosourea-induced retinal photoreceptor apoptosis in vivo. Indian J Pharmacol. 45:149–154. 2013. View Article : Google Scholar : PubMed/NCBI

31 

Ambhore N, Prasanna M, Antony A, Satish Kumar M and Elango K: Pharmacological and anti-oxidant evaluation of Aspirin, nimodipine and its combination for anti-Parkinson's activity in MPTP induced rat model. Int J Health Allied Sci. 3:142014.

32 

Yu L, Wang N, Zhang Y, Wang Y, Li J, Wu Q and Liu Y: Neuroprotective effect of muscone on glutamate-induced apoptosis in PC12 cells via antioxidant and Ca(2+) antagonism. Neurochem Int. 70:10–21. 2014. View Article : Google Scholar : PubMed/NCBI

33 

Amin KK and Shah A: Prevention of oxidative stress and cell death in human lens epithelial cells by guggulsterone. Invest Ophthalmol Vis Sci. 53:1070. 2012.

34 

Dent P, Yacoub A, Fisher PB, Hagan MP and Grant S: MAPK pathways in radiation responses. Oncogene. 22:5885–5896. 2003. View Article : Google Scholar : PubMed/NCBI

35 

Li T, Dai W and Lu L: Ultraviolet-induced junD activation and apoptosis in myeloblastic leukemia ML-1 cells. J Biol Chem. 277:32668–32676. 2002. View Article : Google Scholar : PubMed/NCBI

36 

Wang CP, Li GC, Shi YW, Zhang XC, Li JL, Wang ZW, Ding F and Liang XM: Neuroprotective effect of schizandrin A on oxygen and glucose deprivation/reperfusion-induced cell injury in primary culture of rat cortical neurons. J Physiol Biochem. 70:735–747. 2014. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Jia Y, Qin Q, Fang CP, Shen W, Sun TT, Huang YL, Li WJ and Deng AM: UVB induces apoptosis via downregulation of CALML3-dependent JNK1/2 and ERK1/2 pathways in cataract. Int J Mol Med 41: 3041-3050, 2018.
APA
Jia, Y., Qin, Q., Fang, C., Shen, W., Sun, T., Huang, Y. ... Deng, A. (2018). UVB induces apoptosis via downregulation of CALML3-dependent JNK1/2 and ERK1/2 pathways in cataract. International Journal of Molecular Medicine, 41, 3041-3050. https://doi.org/10.3892/ijmm.2018.3478
MLA
Jia, Y., Qin, Q., Fang, C., Shen, W., Sun, T., Huang, Y., Li, W., Deng, A."UVB induces apoptosis via downregulation of CALML3-dependent JNK1/2 and ERK1/2 pathways in cataract". International Journal of Molecular Medicine 41.5 (2018): 3041-3050.
Chicago
Jia, Y., Qin, Q., Fang, C., Shen, W., Sun, T., Huang, Y., Li, W., Deng, A."UVB induces apoptosis via downregulation of CALML3-dependent JNK1/2 and ERK1/2 pathways in cataract". International Journal of Molecular Medicine 41, no. 5 (2018): 3041-3050. https://doi.org/10.3892/ijmm.2018.3478
Copy and paste a formatted citation
x
Spandidos Publications style
Jia Y, Qin Q, Fang CP, Shen W, Sun TT, Huang YL, Li WJ and Deng AM: UVB induces apoptosis via downregulation of CALML3-dependent JNK1/2 and ERK1/2 pathways in cataract. Int J Mol Med 41: 3041-3050, 2018.
APA
Jia, Y., Qin, Q., Fang, C., Shen, W., Sun, T., Huang, Y. ... Deng, A. (2018). UVB induces apoptosis via downregulation of CALML3-dependent JNK1/2 and ERK1/2 pathways in cataract. International Journal of Molecular Medicine, 41, 3041-3050. https://doi.org/10.3892/ijmm.2018.3478
MLA
Jia, Y., Qin, Q., Fang, C., Shen, W., Sun, T., Huang, Y., Li, W., Deng, A."UVB induces apoptosis via downregulation of CALML3-dependent JNK1/2 and ERK1/2 pathways in cataract". International Journal of Molecular Medicine 41.5 (2018): 3041-3050.
Chicago
Jia, Y., Qin, Q., Fang, C., Shen, W., Sun, T., Huang, Y., Li, W., Deng, A."UVB induces apoptosis via downregulation of CALML3-dependent JNK1/2 and ERK1/2 pathways in cataract". International Journal of Molecular Medicine 41, no. 5 (2018): 3041-3050. https://doi.org/10.3892/ijmm.2018.3478
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team