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Tracking of transplanted human umbilical cord-derived mesenchymal stem cells labeled with fluorescent probe in a mouse model of acute lung injury

  • Authors:
    • Genglong Liu
    • Haijin Lv
    • Yuling An
    • Xuxia Wei
    • Xiaomeng Yi
    • Huimin Yi
  • View Affiliations / Copyright

    Affiliations: Surgical Intensive Care Unit, The Third Affiliated Hospital of Sun Yat‑sen University, Guangzhou, Guangdong 510630, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2527-2534
    |
    Published online on: February 13, 2018
       https://doi.org/10.3892/ijmm.2018.3491
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Abstract

The aim of the present study was topreliminarily visualize the distribution of humanumbilical cord‑derivedmesenchymal stem cells (hUC‑MSCs) in treating acute lung injury (ALI) using a targeted fluorescent technique. Anovel fluorescent molecule probe was first synthesized via the specific binding of antigen and antibody in vitro to label the hUC‑MSCs. Two groups of mice, comprising a normal saline (NS)+MSC group and lipopolysaccharide (LPS)+MSC group, were subjected to optical imaging. At 4 h following ALI mouse model construction, the labeled hUC‑MSCs were transplanted into the mice in the NS+MSC group and LPS+MSC group by tail vein injection. The mice were sacrificed 30 min, 1 day, 3 days and 7 days following injection of the labeled hUC‑MSCs, and the lungs, heart, spleen, kidneys and liver were removed. The excised lungs, heart, spleen, kidneys and liver were then detected on asmall animal fluorescent imager. The fluorescent results showed that the signal intensity in the lungs of the LPS+MSC group was significantly higher, compared with that of the NS+MSC group at 30 min (3.53±0.06x10‑4, vs. 1.95±0.05x10‑4 scaled counts/sec), 1 day (36.20±0.77x10‑4, vs. 23.45±0.43x10‑4 scaled counts/sec), 3 days (11.83±0.26x10‑4, vs. 5.39±0.10x10‑4 scaled counts/sec), and 7 days (3.14±0.04x10‑4, vs. 0.00±0.00x10‑4 scaled counts/sec; all P<0.05). The fluorescence intensity in the liver of the LPS+MSC group, vs. NS+MSC group was measured at 30 min (0.00±0.00x10‑4, vs. 0.00±0.00x10‑4 scaled counts/sec); 1 day (5.53±0.08x10‑4, vs. 5.44±0.16x10‑4 scaled counts/sec); 3 days (0.00±0.00x10‑4, vs. 8.67±0.05x10‑4 scaled counts/sec); 7 days (0.00±0.00x10‑4, vs. 0.00±0.00x10‑4 scaled counts/sec). The signal intensity of the heart, spleen and kidneys was minimal. In conclusion, the novel targeted fluorescence molecular probe was suitable for tracking the distribution processes of hUC‑MSCs in treating ALI.
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Copy and paste a formatted citation
Spandidos Publications style
Liu G, Lv H, An Y, Wei X, Yi X and Yi H: Tracking of transplanted human umbilical cord-derived mesenchymal stem cells labeled with fluorescent probe in a mouse model of acute lung injury. Int J Mol Med 41: 2527-2534, 2018.
APA
Liu, G., Lv, H., An, Y., Wei, X., Yi, X., & Yi, H. (2018). Tracking of transplanted human umbilical cord-derived mesenchymal stem cells labeled with fluorescent probe in a mouse model of acute lung injury. International Journal of Molecular Medicine, 41, 2527-2534. https://doi.org/10.3892/ijmm.2018.3491
MLA
Liu, G., Lv, H., An, Y., Wei, X., Yi, X., Yi, H."Tracking of transplanted human umbilical cord-derived mesenchymal stem cells labeled with fluorescent probe in a mouse model of acute lung injury". International Journal of Molecular Medicine 41.5 (2018): 2527-2534.
Chicago
Liu, G., Lv, H., An, Y., Wei, X., Yi, X., Yi, H."Tracking of transplanted human umbilical cord-derived mesenchymal stem cells labeled with fluorescent probe in a mouse model of acute lung injury". International Journal of Molecular Medicine 41, no. 5 (2018): 2527-2534. https://doi.org/10.3892/ijmm.2018.3491
Copy and paste a formatted citation
x
Spandidos Publications style
Liu G, Lv H, An Y, Wei X, Yi X and Yi H: Tracking of transplanted human umbilical cord-derived mesenchymal stem cells labeled with fluorescent probe in a mouse model of acute lung injury. Int J Mol Med 41: 2527-2534, 2018.
APA
Liu, G., Lv, H., An, Y., Wei, X., Yi, X., & Yi, H. (2018). Tracking of transplanted human umbilical cord-derived mesenchymal stem cells labeled with fluorescent probe in a mouse model of acute lung injury. International Journal of Molecular Medicine, 41, 2527-2534. https://doi.org/10.3892/ijmm.2018.3491
MLA
Liu, G., Lv, H., An, Y., Wei, X., Yi, X., Yi, H."Tracking of transplanted human umbilical cord-derived mesenchymal stem cells labeled with fluorescent probe in a mouse model of acute lung injury". International Journal of Molecular Medicine 41.5 (2018): 2527-2534.
Chicago
Liu, G., Lv, H., An, Y., Wei, X., Yi, X., Yi, H."Tracking of transplanted human umbilical cord-derived mesenchymal stem cells labeled with fluorescent probe in a mouse model of acute lung injury". International Journal of Molecular Medicine 41, no. 5 (2018): 2527-2534. https://doi.org/10.3892/ijmm.2018.3491
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