Open Access

Downregulated miR-23b-3p expression acts as a predictor of hepatocellular carcinoma progression: A study based on public data and RT-qPCR verification

  • Authors:
    • Rong-Quan He
    • Pei-Rong Wu
    • Xue-Lian Xiang
    • Xia Yang
    • Hai-Wei Liang
    • Xiao-Hui Qiu
    • Li-Hua Yang
    • Zhi-Gang Peng
    • Gang Chen
  • View Affiliations

  • Published online on: February 23, 2018     https://doi.org/10.3892/ijmm.2018.3513
  • Pages: 2813-2831
  • Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Mounting evidence has shown that miR-23b-3p, which is associated with cell proliferation, invasion, and apoptosis, acts as a biomarker for diagnosis and outcomes in numerous cancers. However, the clinicopathological implication of miR-23b-3p in hepatocellular carcinoma (HCC) remains unclear. Our study evaluated the role of miR-23b-3p in HCC and investigated its potential application as a marker for preliminary diagnosis and therapy in HCC. High-throughput data from the NCBI Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) were collected and analyzed. One hundred and one tissue sections of HCC were paired with adjacent non-cancerous HCC as further supplements. miR-23b-3p expression was detected using quantitative real-time PCR. Additionally, the relationship between miR-23b-3p expression and HCC progression and Time-to-recurrence (months) was explored. Ten algorithms were applied to predict the prospective target genes of miR-23b-3p. Next, we conducted bioinformatics analysis for further study. miR-23b-3p expression was pronouncedly decreased in HCC tissues in contrast with their paired adjacent non-cancerous HCC (P<0.001) with RT-qPCR. In total, 405 targets, acquired with consistent prediction from at least five databases, were used for the bioinformatics analysis. According to the Gene Ontology (GO) analysis, all targets were classified into biological processes, cellular components and molecular functions. In the pathway analysis, targets of miR-23b-3p were primarily enriched in the signaling pathways of renal cell carcinoma, hepatitis B and pancreatic cancer (corrected P-value <0.05). In the protein-protein interaction (PPI) network for miR-23b-3p, a total of 8 targets, including SRC, AKT1, EGFR, CTNNB1, BCL2, SMAD3, PTEN and KDM6A, were located in the key nodes with high degree (>35). In conclusion, this study provides impressive illumination of the potential role of miR-23b-3p in HCC tumorigenesis and progression. Furthermore, miR-23b-3p may act as a predictor of HCC and could be a new treatment target.
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May-2018
Volume 41 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
He R, Wu P, Xiang X, Yang X, Liang H, Qiu X, Yang L, Peng Z and Chen G: Downregulated miR-23b-3p expression acts as a predictor of hepatocellular carcinoma progression: A study based on public data and RT-qPCR verification. Int J Mol Med 41: 2813-2831, 2018
APA
He, R., Wu, P., Xiang, X., Yang, X., Liang, H., Qiu, X. ... Chen, G. (2018). Downregulated miR-23b-3p expression acts as a predictor of hepatocellular carcinoma progression: A study based on public data and RT-qPCR verification. International Journal of Molecular Medicine, 41, 2813-2831. https://doi.org/10.3892/ijmm.2018.3513
MLA
He, R., Wu, P., Xiang, X., Yang, X., Liang, H., Qiu, X., Yang, L., Peng, Z., Chen, G."Downregulated miR-23b-3p expression acts as a predictor of hepatocellular carcinoma progression: A study based on public data and RT-qPCR verification". International Journal of Molecular Medicine 41.5 (2018): 2813-2831.
Chicago
He, R., Wu, P., Xiang, X., Yang, X., Liang, H., Qiu, X., Yang, L., Peng, Z., Chen, G."Downregulated miR-23b-3p expression acts as a predictor of hepatocellular carcinoma progression: A study based on public data and RT-qPCR verification". International Journal of Molecular Medicine 41, no. 5 (2018): 2813-2831. https://doi.org/10.3892/ijmm.2018.3513