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miR‑377 targets CUL4A and regulates metastatic capability in ovarian cancer

  • Authors:
    • Rufen Yu
    • Limei Cai
    • Yingui Chi
    • Xiangcui Ding
    • Xueqing Wu
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Ruian People's Hospital, The Third Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325200, P. R. China, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Wenzhou Medical University, The First Provincial Wenzhou Hospital of Zhejiang, Wenzhou, Zhejiang 325000, P. R. China
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3147-3156
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    Published online on: March 7, 2018
       https://doi.org/10.3892/ijmm.2018.3540
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Abstract

The incidence and recurrence rates of ovarian cancer are still high, and once the disease metastasizes, it is nearly always fatal. Cullin 4A (CUL4A) serves a significant role in tumourigenesis and tumour progression; however, the effect and mechanisms underlying CUL4A overexpression are still unknown. The role of microRNAs (miRs) in the regulation of metastatic capability in ovarian cancer cell lines was investigated. The interaction between miR‑377 and CUL4A was investigated using bioinformatics analyses and dual‑luciferase reporter assays. Furthermore, miR‑377 mRNA and protein levels were detected using reverse transcription‑quantitative polymerase chain reaction and western blotting, respectively and cell migration and invasion were detected using a Transwell assay. Results revealed that CUL4A expression was negatively associated with miR‑377 levels in ovarian cancer tissues and cell lines. Through in silico analysis, the targeting effect of miR‑377 on CUL4A was verified. Ectopic expression of miR‑377 in SKOV3 cells downregulated the level of CUL4A, and significantly reduced the migratory ability of the cells. miR‑377 overexpression led to reduced activity of the Wnt/β‑catenin signaling pathway, and regulated the expression of matrix metalloproteinase‑2, and 9, and epithelial‑mesenchymal transition (EMT)‑associated protein. These results suggested that miR‑377 is a significant negative regulator of CUL4A that controls cancer cell progression in ovarian cancer cell lines.
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Copy and paste a formatted citation
Spandidos Publications style
Yu R, Cai L, Chi Y, Ding X and Wu X: miR‑377 targets CUL4A and regulates metastatic capability in ovarian cancer. Int J Mol Med 41: 3147-3156, 2018.
APA
Yu, R., Cai, L., Chi, Y., Ding, X., & Wu, X. (2018). miR‑377 targets CUL4A and regulates metastatic capability in ovarian cancer. International Journal of Molecular Medicine, 41, 3147-3156. https://doi.org/10.3892/ijmm.2018.3540
MLA
Yu, R., Cai, L., Chi, Y., Ding, X., Wu, X."miR‑377 targets CUL4A and regulates metastatic capability in ovarian cancer". International Journal of Molecular Medicine 41.6 (2018): 3147-3156.
Chicago
Yu, R., Cai, L., Chi, Y., Ding, X., Wu, X."miR‑377 targets CUL4A and regulates metastatic capability in ovarian cancer". International Journal of Molecular Medicine 41, no. 6 (2018): 3147-3156. https://doi.org/10.3892/ijmm.2018.3540
Copy and paste a formatted citation
x
Spandidos Publications style
Yu R, Cai L, Chi Y, Ding X and Wu X: miR‑377 targets CUL4A and regulates metastatic capability in ovarian cancer. Int J Mol Med 41: 3147-3156, 2018.
APA
Yu, R., Cai, L., Chi, Y., Ding, X., & Wu, X. (2018). miR‑377 targets CUL4A and regulates metastatic capability in ovarian cancer. International Journal of Molecular Medicine, 41, 3147-3156. https://doi.org/10.3892/ijmm.2018.3540
MLA
Yu, R., Cai, L., Chi, Y., Ding, X., Wu, X."miR‑377 targets CUL4A and regulates metastatic capability in ovarian cancer". International Journal of Molecular Medicine 41.6 (2018): 3147-3156.
Chicago
Yu, R., Cai, L., Chi, Y., Ding, X., Wu, X."miR‑377 targets CUL4A and regulates metastatic capability in ovarian cancer". International Journal of Molecular Medicine 41, no. 6 (2018): 3147-3156. https://doi.org/10.3892/ijmm.2018.3540
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