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Article

RP105 ameliorates hypoxia̸reoxygenation injury in cardiac microvascular endothelial cells by suppressing TLR4̸MAPKs̸NF-κB signaling

  • Authors:
    • Xin Guo
    • Hong Jiang
    • Jing Chen
    • Bo-Fang Zhang
    • Qi Hu
    • Shuo Yang
    • Jian Yang
    • Jing Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Renmin Hospital of Wuhan University, Cardiovascular Research Institute of Wuhan University, Wuhan, Hubei 430060, P.R. China, Department of Cardiology, The First College of Clinical Medical Sciences, China Three Gorges University, Yichang, Hubei 443000, P.R. China
  • Pages: 505-513
    |
    Published online on: April 16, 2018
       https://doi.org/10.3892/ijmm.2018.3621
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Abstract

The radioprotective 105 kDa protein (RP105) has been implicated in the pathological process of multiple cardiovascular diseases through its functional and physical interactions with Toll‑like receptor 4 (TLR4). However, the effects of RP105 on cardiac microvascular endothelial cells (CMECs) in response to hypoxia̸reoxygenation (H̸R) injury have not been extensively investigated. The aim of the present study was to elucidate the potential roles of RP105 in the protection of CMECs against H̸R injury, and investigate the underlying mechanisms. CMECs isolated from Sprague‑Dawley rats were transduced with adenoviral vectors encoding RP105 or green fluorescent protein (GFP). At 48 h post‑transfection, CMECs were subjected to hypoxia for 4 h and reoxygenation for 2 h (H̸R) to simulate the in vivo ischemia̸reperfusion model. The mRNA and protein levels of RP105 were detected by reverse transcription‑quantitative polymerase chain reaction and western blot analysis, respectively. The effects of RP105 on CMEC proliferation, migration and apoptosis were measured by GFP‑8, Transwell chamber and flow cytometry assays, respectively. The secretion of interleukin (IL)‑6 and tumor necrosis factor (TNF)‑α in the culture medium was measured by ELISA. Moreover, the expression level of TLR4, p38 mitogen‑activated protein kinase (MAPK), extracellular-signal-regulated kinase 1̸2, c-Jun N-terminal kinase, nuclear factor (NF)‑κB̸p65, IL‑6, TNF‑α and intercellular adhesion melecule‑1 was evaluated by western blot analysis. The results demonstrated that RP105 was minimally expressed in CMECs subjected to H̸R injury. Overexpression of RP105 via adenoviral vectors was able to significantly protect CMECs against H̸R injury, as evidenced by the promotion of cell proliferation and migration, as well as the amelioration of inflammation and apoptosis. These beneficial effects were at least partly mediated through inhibition of TLR4̸MAPKs̸NF‑κB signaling. Therefore, RP105 may be a promising candidate for prevention against CMECs‑associated H̸R injury.
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Copy and paste a formatted citation
Spandidos Publications style
Guo X, Jiang H, Chen J, Zhang B, Hu Q, Yang S, Yang J and Zhang J: RP105 ameliorates hypoxia̸reoxygenation injury in cardiac microvascular endothelial cells by suppressing TLR4̸MAPKs̸NF-κB signaling. Int J Mol Med 42: 505-513, 2018.
APA
Guo, X., Jiang, H., Chen, J., Zhang, B., Hu, Q., Yang, S. ... Zhang, J. (2018). RP105 ameliorates hypoxia̸reoxygenation injury in cardiac microvascular endothelial cells by suppressing TLR4̸MAPKs̸NF-κB signaling. International Journal of Molecular Medicine, 42, 505-513. https://doi.org/10.3892/ijmm.2018.3621
MLA
Guo, X., Jiang, H., Chen, J., Zhang, B., Hu, Q., Yang, S., Yang, J., Zhang, J."RP105 ameliorates hypoxia̸reoxygenation injury in cardiac microvascular endothelial cells by suppressing TLR4̸MAPKs̸NF-κB signaling". International Journal of Molecular Medicine 42.1 (2018): 505-513.
Chicago
Guo, X., Jiang, H., Chen, J., Zhang, B., Hu, Q., Yang, S., Yang, J., Zhang, J."RP105 ameliorates hypoxia̸reoxygenation injury in cardiac microvascular endothelial cells by suppressing TLR4̸MAPKs̸NF-κB signaling". International Journal of Molecular Medicine 42, no. 1 (2018): 505-513. https://doi.org/10.3892/ijmm.2018.3621
Copy and paste a formatted citation
x
Spandidos Publications style
Guo X, Jiang H, Chen J, Zhang B, Hu Q, Yang S, Yang J and Zhang J: RP105 ameliorates hypoxia̸reoxygenation injury in cardiac microvascular endothelial cells by suppressing TLR4̸MAPKs̸NF-κB signaling. Int J Mol Med 42: 505-513, 2018.
APA
Guo, X., Jiang, H., Chen, J., Zhang, B., Hu, Q., Yang, S. ... Zhang, J. (2018). RP105 ameliorates hypoxia̸reoxygenation injury in cardiac microvascular endothelial cells by suppressing TLR4̸MAPKs̸NF-κB signaling. International Journal of Molecular Medicine, 42, 505-513. https://doi.org/10.3892/ijmm.2018.3621
MLA
Guo, X., Jiang, H., Chen, J., Zhang, B., Hu, Q., Yang, S., Yang, J., Zhang, J."RP105 ameliorates hypoxia̸reoxygenation injury in cardiac microvascular endothelial cells by suppressing TLR4̸MAPKs̸NF-κB signaling". International Journal of Molecular Medicine 42.1 (2018): 505-513.
Chicago
Guo, X., Jiang, H., Chen, J., Zhang, B., Hu, Q., Yang, S., Yang, J., Zhang, J."RP105 ameliorates hypoxia̸reoxygenation injury in cardiac microvascular endothelial cells by suppressing TLR4̸MAPKs̸NF-κB signaling". International Journal of Molecular Medicine 42, no. 1 (2018): 505-513. https://doi.org/10.3892/ijmm.2018.3621
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