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Enhanced proliferation and differentiation of HO-1 gene-modified bone marrow-derived mesenchymal stem cells in the acute injured kidney

  • Authors:
    • Nanmei Liu
    • Huiling Wang
    • Guofeng Han
    • Jin Cheng
    • Weifeng Hu
    • Jinyuan Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, The 455th Hospital of PLA, Shanghai 200052, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 946-956
    |
    Published online on: May 11, 2018
       https://doi.org/10.3892/ijmm.2018.3670
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Abstract

The aim of the present study was to investigate the effect of heme oxygenase-1 (HO-1) overexpression on the survival and differentiation ability of bone marrow‑derived mesenchymal stem cells (BMSCs) in the acute kidney injury (AKI) microenvironment. HO-1-BMSCs and enhanced green fluorescent protein (eGFP)-BMSCs were constructed. Rat ischemia/reperfusion (I/R)‑AKI-kidney homogenate supernatant was prep­ared to treat the BMSCs, eGFP-BMSCs and HO-1-BMSCs in vitro. In the AKI microenvironment, the HO-1-BMSCs exhibited a smaller proportion of cells at the G0/G1 phase, and a larger proportion of cells expressing proliferating cell nuclear antigen (PCNA) and cytokeratin 18 (CK18). Phosphorylated protein kinase B (Akt) and extracellular signal‑regulated kinase (ERK) protein levels were observed to be increased in the HO-1-BMSCs compared with the BMSCs. LY294002 and PD98059 each inhibited the above effects. BMSCs, eGFP-BMSCs and HO-1-BMSCs were implanted into an I/R-AKI rat model. The proportions of PCNA+ BMSCs and CK18+ BMSCs were higher in the HO-1-BMSCs group compared with the BMSCs group, which resulted in a decreased acute tubular necrosis score and improved renal function for the AKI rats. In conclusion, the enhanced proliferation and differentiation of HO-1-BMSCs suggest the beneficial effects of such cells in the BMSC-based therapy of AKI. The mechanism underlying these effects may involve the stimulation of Akt and ERK signaling.
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Copy and paste a formatted citation
Spandidos Publications style
Liu N, Wang H, Han G, Cheng J, Hu W and Zhang J: Enhanced proliferation and differentiation of HO-1 gene-modified bone marrow-derived mesenchymal stem cells in the acute injured kidney. Int J Mol Med 42: 946-956, 2018.
APA
Liu, N., Wang, H., Han, G., Cheng, J., Hu, W., & Zhang, J. (2018). Enhanced proliferation and differentiation of HO-1 gene-modified bone marrow-derived mesenchymal stem cells in the acute injured kidney. International Journal of Molecular Medicine, 42, 946-956. https://doi.org/10.3892/ijmm.2018.3670
MLA
Liu, N., Wang, H., Han, G., Cheng, J., Hu, W., Zhang, J."Enhanced proliferation and differentiation of HO-1 gene-modified bone marrow-derived mesenchymal stem cells in the acute injured kidney". International Journal of Molecular Medicine 42.2 (2018): 946-956.
Chicago
Liu, N., Wang, H., Han, G., Cheng, J., Hu, W., Zhang, J."Enhanced proliferation and differentiation of HO-1 gene-modified bone marrow-derived mesenchymal stem cells in the acute injured kidney". International Journal of Molecular Medicine 42, no. 2 (2018): 946-956. https://doi.org/10.3892/ijmm.2018.3670
Copy and paste a formatted citation
x
Spandidos Publications style
Liu N, Wang H, Han G, Cheng J, Hu W and Zhang J: Enhanced proliferation and differentiation of HO-1 gene-modified bone marrow-derived mesenchymal stem cells in the acute injured kidney. Int J Mol Med 42: 946-956, 2018.
APA
Liu, N., Wang, H., Han, G., Cheng, J., Hu, W., & Zhang, J. (2018). Enhanced proliferation and differentiation of HO-1 gene-modified bone marrow-derived mesenchymal stem cells in the acute injured kidney. International Journal of Molecular Medicine, 42, 946-956. https://doi.org/10.3892/ijmm.2018.3670
MLA
Liu, N., Wang, H., Han, G., Cheng, J., Hu, W., Zhang, J."Enhanced proliferation and differentiation of HO-1 gene-modified bone marrow-derived mesenchymal stem cells in the acute injured kidney". International Journal of Molecular Medicine 42.2 (2018): 946-956.
Chicago
Liu, N., Wang, H., Han, G., Cheng, J., Hu, W., Zhang, J."Enhanced proliferation and differentiation of HO-1 gene-modified bone marrow-derived mesenchymal stem cells in the acute injured kidney". International Journal of Molecular Medicine 42, no. 2 (2018): 946-956. https://doi.org/10.3892/ijmm.2018.3670
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