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Article

BMP‑6 protects retinal pigment epithelial cells from oxidative stress‑induced injury by inhibiting the MAPK signaling pathways

  • Authors:
    • Li Chen
    • Ming Liu
    • Yan Luan
    • Yingfei Liu
    • Zhichao Zhang
    • Bo Ma
    • Xuan Liu
    • Yong Liu
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Department of Ophthalmology, The First Hospital of Xi'an, Xi'an, Shaanxi 710002, P.R. China, Institute of Neurobiology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, P.R. China
  • Pages: 1096-1105
    |
    Published online on: May 14, 2018
       https://doi.org/10.3892/ijmm.2018.3675
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Abstract

Worldwide, neovascular age‑related macular degeneration (nAMD) is one of the most common causes of blindness in the elderly. In particular, degeneration of retinal pigment epithelial (RPE) cells represents the main pathological process in the development of nAMD, and oxidative stress serves a major role. The present study aimed to investigate the association between bone morphogenetic protein 6 (BMP‑6) and nAMD. BMP‑6 concentration was significantly reduced in patients with wet nAMD compared with in the control group. Furthermore, the present study investigated the protective effects of BMP‑6 on RPE cells following oxidative stress‑induced injury. Cell Counting Kit‑8 assay and terminal deoxynucleotidyl transferase dUTP nick‑end labeling staining demonstrated that BMP‑6 increased RPE cell viability, which was decreased following treatment with hydrogen peroxide (H2O2), and reduced H2O2‑induced apoptosis. In addition, western blotting revealed that BMP‑6 reversed the decrease in pro‑caspase‑3 levels and the dysregulation of the B‑cell lymphoma 2 (Bcl‑2)/Bcl‑2‑associated X protein (Bax) balance caused by H2O2. In addition, alterations in c‑Jun N‑terminal protein kinase (JNK) and p38 mitogen‑activated protein kinase (MAPK) expression were examined, and pretreatment with BMP‑6 was demonstrated to reduce H2O2‑induced activation of JNK and p38 MAPK. Conversely, the effects of BMP‑6 were attenuated by its inhibitor noggin. In conclusion, the present study demonstrated that BMP‑6 may protect RPE cells from oxidative stress injury to a certain extent, which may be associated with alterations in the MAPK signaling pathway. However, the specific mechanism of action underlying this effect requires further investigation. Overall, the present study laid a foundation for exploring novel nAMD treatment methods.
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Copy and paste a formatted citation
Spandidos Publications style
Chen L, Liu M, Luan Y, Liu Y, Zhang Z, Ma B, Liu X and Liu Y: BMP‑6 protects retinal pigment epithelial cells from oxidative stress‑induced injury by inhibiting the MAPK signaling pathways. Int J Mol Med 42: 1096-1105, 2018.
APA
Chen, L., Liu, M., Luan, Y., Liu, Y., Zhang, Z., Ma, B. ... Liu, Y. (2018). BMP‑6 protects retinal pigment epithelial cells from oxidative stress‑induced injury by inhibiting the MAPK signaling pathways. International Journal of Molecular Medicine, 42, 1096-1105. https://doi.org/10.3892/ijmm.2018.3675
MLA
Chen, L., Liu, M., Luan, Y., Liu, Y., Zhang, Z., Ma, B., Liu, X., Liu, Y."BMP‑6 protects retinal pigment epithelial cells from oxidative stress‑induced injury by inhibiting the MAPK signaling pathways". International Journal of Molecular Medicine 42.2 (2018): 1096-1105.
Chicago
Chen, L., Liu, M., Luan, Y., Liu, Y., Zhang, Z., Ma, B., Liu, X., Liu, Y."BMP‑6 protects retinal pigment epithelial cells from oxidative stress‑induced injury by inhibiting the MAPK signaling pathways". International Journal of Molecular Medicine 42, no. 2 (2018): 1096-1105. https://doi.org/10.3892/ijmm.2018.3675
Copy and paste a formatted citation
x
Spandidos Publications style
Chen L, Liu M, Luan Y, Liu Y, Zhang Z, Ma B, Liu X and Liu Y: BMP‑6 protects retinal pigment epithelial cells from oxidative stress‑induced injury by inhibiting the MAPK signaling pathways. Int J Mol Med 42: 1096-1105, 2018.
APA
Chen, L., Liu, M., Luan, Y., Liu, Y., Zhang, Z., Ma, B. ... Liu, Y. (2018). BMP‑6 protects retinal pigment epithelial cells from oxidative stress‑induced injury by inhibiting the MAPK signaling pathways. International Journal of Molecular Medicine, 42, 1096-1105. https://doi.org/10.3892/ijmm.2018.3675
MLA
Chen, L., Liu, M., Luan, Y., Liu, Y., Zhang, Z., Ma, B., Liu, X., Liu, Y."BMP‑6 protects retinal pigment epithelial cells from oxidative stress‑induced injury by inhibiting the MAPK signaling pathways". International Journal of Molecular Medicine 42.2 (2018): 1096-1105.
Chicago
Chen, L., Liu, M., Luan, Y., Liu, Y., Zhang, Z., Ma, B., Liu, X., Liu, Y."BMP‑6 protects retinal pigment epithelial cells from oxidative stress‑induced injury by inhibiting the MAPK signaling pathways". International Journal of Molecular Medicine 42, no. 2 (2018): 1096-1105. https://doi.org/10.3892/ijmm.2018.3675
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