Open Access

Linarin prevents LPS‑induced acute lung injury by suppressing oxidative stress and inflammation via inhibition of TXNIP/NLRP3 and NF‑κB pathways

  • Authors:
    • Xiang Han
    • Yi‑Chen Wu
    • Min Meng
    • Qing‑Song Sun
    • Su‑Min Gao
    • Hong Sun
  • View Affiliations

  • Published online on: May 30, 2018     https://doi.org/10.3892/ijmm.2018.3710
  • Pages: 1460-1472
  • Copyright: © Han et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Acute lung injury (ALI) is an important cause of morbidity and mortality for critically ill patients, and linarin (LR) may be a potential treatment for ALI as it reportedly has antioxidant, anti‑inflammatory and apoptotic‑regulating activity. In the present study, the authors report that saline and LR (12.5, 25 and 50 mg/kg) were applied to male C57BL/6 mice via gavage. Then, mice were intratracheally injected with either saline or lipopolysaccharide (LPS). LR‑pretreatment attenuated LPS‑induced ALI and platelet activation and reduced CD41 expression levels and neutrophil platelet aggregates. Additionally, LPS‑triggered pulmonary myeloperoxidase activity and neutrophil infiltration in lung tissues, and this was eliminated by LR dose‑dependently. Furthermore, LPS‑induced oxidative stress and pro‑inflammatory cytokine release were downregulated by LR by inhibiting thioredoxin‑interacting protein and nuclear factor‑κB signaling pathways, including their downstream and upstream signals, such as xanthine oxidase, NLR family WHAT, pyrin domain‑containing 3 (NLRP3), apoptosis‑associated speck‑like protein containing a C‑terminal caspase recruitment domain (ASC), caspase‑1, IκB kinase‑α (IKK‑α) and IκBα. Moreover, in LPS‑induced mice, the mitogen‑activated protein kinase pathway was inactivated by LR. In vitro, LR reduced LPS‑induced inflammation and oxidative stress, which was linked to reduction of ROS. In conclusion, LR pretreatment may be protective against LPS‑induced ALI.
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September-2018
Volume 42 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Han X, Wu YC, Meng M, Sun QS, Gao SM and Sun H: Linarin prevents LPS‑induced acute lung injury by suppressing oxidative stress and inflammation via inhibition of TXNIP/NLRP3 and NF‑κB pathways. Int J Mol Med 42: 1460-1472, 2018
APA
Han, X., Wu, Y., Meng, M., Sun, Q., Gao, S., & Sun, H. (2018). Linarin prevents LPS‑induced acute lung injury by suppressing oxidative stress and inflammation via inhibition of TXNIP/NLRP3 and NF‑κB pathways. International Journal of Molecular Medicine, 42, 1460-1472. https://doi.org/10.3892/ijmm.2018.3710
MLA
Han, X., Wu, Y., Meng, M., Sun, Q., Gao, S., Sun, H."Linarin prevents LPS‑induced acute lung injury by suppressing oxidative stress and inflammation via inhibition of TXNIP/NLRP3 and NF‑κB pathways". International Journal of Molecular Medicine 42.3 (2018): 1460-1472.
Chicago
Han, X., Wu, Y., Meng, M., Sun, Q., Gao, S., Sun, H."Linarin prevents LPS‑induced acute lung injury by suppressing oxidative stress and inflammation via inhibition of TXNIP/NLRP3 and NF‑κB pathways". International Journal of Molecular Medicine 42, no. 3 (2018): 1460-1472. https://doi.org/10.3892/ijmm.2018.3710