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Article

MicroRNA‑155 contributes to the occurrence of epilepsy through the PI3K/Akt/mTOR signaling pathway

  • Authors:
    • Wei Duan
    • Yan Chen
    • Xiao‑Rong Wang
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, The Fifth Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Department of Neurophysiology, Oilfields General Hospital in Daqing, Daqing, Heilongjiang 163001, P.R. China, Department of Neurology, Ya'an Hospital, Ya'an, Sichuan 625000, P.R. China
  • Pages: 1577-1584
    |
    Published online on: June 1, 2018
       https://doi.org/10.3892/ijmm.2018.3711
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Abstract

Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to measure the expression of microRNA-155 in patients with temporal lobe epilepsy. Commercial kit and western blot analysis were used to measure gap-associated protein expression. The aim of the present study was to investigate the effect of microRNA‑155 (miRNA‑155) in the occurrence of epilepsy and the molecular mechanism involved. In patients with temporal lobe epilepsy, miRNA‑155 expression was evidently higher than that in patients of the normal volunteers group. Overexpression of miRNA‑155 resulted in decreased brain‑derived neurotrophic factor (BDNF) and tropomyosin receptor kinase B (TrkB) protein expression, increased caspase‑3 activity, tumor protein p53 (p53) and apoptosis regulator BAX (Bax) protein expression, and inhibited phosphoinositide 3‑kinase (PI3K), phosphorylated (p‑)protein kinase B (Akt) and p‑mechanistic target of rapamycin (mTOR) protein expression in epilepsy cells. PI3K inhibitor accelerated the effect of miRNA‑155 on the inhibition of BDNF and TrkB protein expression, the promotion of caspase‑3 activity, p53 and Bax protein expression, and the inhibition of PI3K, p‑Akt and p‑mTOR protein expression in epilepsy cells. The present findings indicate that miRNA‑155 contributes to the occurrence of epilepsy through the PI3K/Akt/mTOR signaling pathway.
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1 

Lazzari AA, Dussault PM, Thakore-James M, Gagnon D, Baker E, Davis SA and Houranieh AM: Prevention of bone loss and vertebral fractures in patients with chronic epilepsy - anti-epileptic drug and osteoporosis prevention trial. Epilepsia. 54:1997–2004. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Delger AB, Avakyan GN, Oleinikova OM, Bogomazova MA, Chromych EA and Lagutin IuV: Effects of tenoten on anxiety and depression disorders in patients with epilepsy. Bull Exp Biol Med. 153:704–706. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Jacoby A, Sudell M, Tudur Smith C, Crossley J, Marson AG and Baker GA; SANAD Study Group: Quality-of-life outcomes of initiating treatment with standard and newer antiepileptic drugs in adults with new-onset epilepsy: Findings from the SANAD trial. Epilepsia. 56:460–472. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Jóźwiak S, Kotulska K, Domańska-Pakieła D, Lojszczyk B, Syczewska M, Chmielewski D, Dunin-Wąsowicz D, Kmieć T, Szymkiewicz-Dangel J, Kornacka M, et al: Antiepileptic treatment before the onset of seizures reduces epilepsy severity and risk of mental retardation in infants with tuberous sclerosis complex. Eur J Paediatr Neurol. 15:424–431. 2011. View Article : Google Scholar

5 

French JA, Abou-Khalil BW, Leroy RF, Yacubian EM, Shin P, Hall S, Mansbach H and Nohria V; RESTORE 1/Study 301 Investigators: Randomized, double-blind, placebo-controlled trial of ezogabine (retigabine) in partial epilepsy. Neurology. 76:1555–1563. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Sharma S, Sankhyan N, Gulati S and Agarwala A: Use of the modified Atkins diet for treatment of refractory childhood epilepsy: A randomized controlled trial. Epilepsia. 54:481–486. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Kerr EN and Blackwell MC: Near-transfer effects following working memory intervention (Cogmed) in children with symptomatic epilepsy: An open randomized clinical trial. Epilepsia. 56:1784–1792. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Li MM, Li XM, Zheng XP, Yu JT and Tan L: MicroRNAs dysregulation in epilepsy. Brain Res. 1584:94–104. 2014. View Article : Google Scholar

9 

Ashhab MU, Omran A, Kong H, Gan N, He F, Peng J and Yin F: Expressions of tumor necrosis factor alpha and microRNA-155 in immature rat model of status epilepticus and children with mesial temporal lobe epilepsy. J Mol Neurosci. 51:950–958. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Li Y, Wang J, Jiang C, Zheng G, Lu X and Guo H: Association of the genetic polymorphisms in pre-microRNAs with risk of childhood epilepsy in a Chinese population. Seizure. 40:21–26. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Li MM, Jiang T, Sun Z, Zhang Q, Tan CC, Yu JT and Tan L: Genome-wide microRNA expression profiles in hippocampus of rats with chronic temporal lobe epilepsy. Sci Rep. 4:47342014. View Article : Google Scholar : PubMed/NCBI

12 

Henshall DC: MicroRNA and epilepsy: Profiling, functions and potential clinical applications. Curr Opin Neurol. 27:199–205. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Xue Y, Xie N, Cao L, Zhao X, Jiang H and Chi Z: Diazoxide preconditioning against seizure-induced oxidative injury is via the PI3K/Akt pathway in epileptic rat. Neurosci Lett. 495:130–134. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Xiao Z, Peng J, Yang L, Kong H and Yin F: Interleukin-1β plays a role in the pathogenesis of mesial temporal lobe epilepsy through the PI3K/Akt/mTOR signaling pathway in hippocampal neurons. J Neuroimmunol. 282:110–117. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Zheng H, Wang X, Tang Z, Zheng W and Li Z: The PI3K/Akt and ERK1/2 signaling pathways mediate the erythropoietin-modulated calcium influx in kainic acid-induced epilepsy. Neuroreport. 24:335–341. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Wong M: mTOR strikes again: mTORC1 activation causes epilepsy independent of overt pathological changes. Epilepsy Curr. 14:41–43. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Bockaert J and Marin P: mTOR in brain physiology and pathologies. Physiol Rev. 95:1157–1187. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar

19 

Aalbers MW, Klinkenberg S, Rijkers K, Verschuure P, Kessels A, Aldenkamp A, Vles J and Majoie M: The effects of vagus nerve stimulation on pro- and anti-inflammatory cytokines in children with refractory epilepsy: An exploratory study. Neuroimmunomodulation. 19:352–358. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Meurer WJ, Silbergleit R, Nicholas KS, Burke JF and Durkalski V: Accounting for repeat enrollments during an emergency clinical trial: The Rapid Anticonvulsant Medications Prior to Arrival Trial (RAMPART). Acad Emerg Med. 22:373–377. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Ryvlin P, Gilliam FG, Nguyen DK, Colicchio G, Iudice A, Tinuper P, Zamponi N, Aguglia U, Wagner L, Minotti L, et al: The long-term effect of vagus nerve stimulation on quality of life in patients with pharmacoresistant focal epilepsy: The PuLsE (Open Prospective Randomized Long-term Effectiveness) trial. Epilepsia. 55:893–900. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Hu K, Xie YY, Zhang C, Ouyang DS, Long HY, Sun DN, Long LL, Feng L, Li Y and Xiao B: MicroRNA expression profile of the hippocampus in a rat model of temporal lobe epilepsy and miR-34a-targeted neuroprotection against hippocampal neurone cell apoptosis post-status epilepticus. BMC Neurosci. 13:1152012. View Article : Google Scholar : PubMed/NCBI

23 

Zucchini S, Marucci G, Paradiso B, Lanza G, Roncon P, Cifelli P, Ferracin M, Giulioni M, Michelucci R, Rubboli G, et al: Identification of miRNAs differentially expressed in human epilepsy with or without granule cell pathology. PLoS One. 9:e1055212014. View Article : Google Scholar : PubMed/NCBI

24 

Reschke CR and Henshall DC: microRNA and Epilepsy. Adv Exp Med Biol. 888:41–70. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Manna I, Labate A, Borzì G, Mumoli L, Cavalli SM, Sturniolo M, Quattrone A and Gambardella A: An SNP site in primiR-124, a brain expressed miRNA gene, no contribution to mesial temporal lobe epilepsy in an Italian sample. Neurol Sci. 37:1335–1339. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Tzeng TT, Tsay HJ, Chang L, Hsu CL, Lai TH, Huang FL and Shiao YJ: Caspase 3 involves in neuroplasticity, microglial activation and neurogenesis in the mice hippocampus after intracerebral injection of kainic acid. J Biomed Sci. 20:902013. View Article : Google Scholar : PubMed/NCBI

27 

Aanandhi MV, Bhattacherjee D, Ray A and George PS: New avenue in the treatment of temporal lobe epilepsy by classical anti-epileptics: A hypothetical establishment of executioner Caspase 3 inactivation by molecular modeling. J Adv Pharm Technol Res. 6:65–74. 2015. View Article : Google Scholar : PubMed/NCBI

28 

Meng XJ, Wang F and Li CK: Resveratrol is neuroprotective and improves cognition in pentylenetetrazole-kindling model of epilepsy in rats. Indian J Pharm Sci. 76:125–131. 2014.PubMed/NCBI

29 

Chen X, Bao G, Hua Y, Li Y, Wang Z and Zhang X: The effects of topiramate on caspase-3 expression in hippocampus of baso-lateral amygdala (BLA) electrical kindled epilepsy rat. J Mol Neurosci. 38:201–206. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Zhang B and Wong M: Pentylenetetrazole-induced seizures cause acute, but not chronic, mTOR pathway activation in rat. Epilepsia. 53:506–511. 2012. View Article : Google Scholar : PubMed/NCBI

31 

Jansen LA, Mirzaa GM, Ishak GE, O'Roak BJ, Hiatt JB, Roden WH, Gunter SA, Christian SL, Collins S, Adams C, et al: PI3K/AKT pathway mutations cause a spectrum of brain malformations from megalencephaly to focal cortical dysplasia. Brain. 138:1613–1628. 2015. View Article : Google Scholar : PubMed/NCBI

32 

Avet-Rochex A, Carvajal N, Christoforou CP, Yeung K, Maierbrugger KT, Hobbs C, Lalli G, Cagin U, Plachot C, McNeill H, et al: Unkempt is negatively regulated by mTOR and uncouples neuronal differentiation from growth control. PLoS Genet. 10:e10046242014. View Article : Google Scholar : PubMed/NCBI

33 

Ye M, Bi YF, Ding L, Zhu WW and Gao W: Saikosaponin a functions as anti-epileptic effect in pentylenetetrazol induced rats through inhibiting mTOR signaling pathway. Biomed Pharmacother. 81:281–287. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Duan W, Chen Y and Wang XR: MicroRNA‑155 contributes to the occurrence of epilepsy through the PI3K/Akt/mTOR signaling pathway. Int J Mol Med 42: 1577-1584, 2018.
APA
Duan, W., Chen, Y., & Wang, X. (2018). MicroRNA‑155 contributes to the occurrence of epilepsy through the PI3K/Akt/mTOR signaling pathway. International Journal of Molecular Medicine, 42, 1577-1584. https://doi.org/10.3892/ijmm.2018.3711
MLA
Duan, W., Chen, Y., Wang, X."MicroRNA‑155 contributes to the occurrence of epilepsy through the PI3K/Akt/mTOR signaling pathway". International Journal of Molecular Medicine 42.3 (2018): 1577-1584.
Chicago
Duan, W., Chen, Y., Wang, X."MicroRNA‑155 contributes to the occurrence of epilepsy through the PI3K/Akt/mTOR signaling pathway". International Journal of Molecular Medicine 42, no. 3 (2018): 1577-1584. https://doi.org/10.3892/ijmm.2018.3711
Copy and paste a formatted citation
x
Spandidos Publications style
Duan W, Chen Y and Wang XR: MicroRNA‑155 contributes to the occurrence of epilepsy through the PI3K/Akt/mTOR signaling pathway. Int J Mol Med 42: 1577-1584, 2018.
APA
Duan, W., Chen, Y., & Wang, X. (2018). MicroRNA‑155 contributes to the occurrence of epilepsy through the PI3K/Akt/mTOR signaling pathway. International Journal of Molecular Medicine, 42, 1577-1584. https://doi.org/10.3892/ijmm.2018.3711
MLA
Duan, W., Chen, Y., Wang, X."MicroRNA‑155 contributes to the occurrence of epilepsy through the PI3K/Akt/mTOR signaling pathway". International Journal of Molecular Medicine 42.3 (2018): 1577-1584.
Chicago
Duan, W., Chen, Y., Wang, X."MicroRNA‑155 contributes to the occurrence of epilepsy through the PI3K/Akt/mTOR signaling pathway". International Journal of Molecular Medicine 42, no. 3 (2018): 1577-1584. https://doi.org/10.3892/ijmm.2018.3711
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