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International Journal of Molecular Medicine
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September-2018 Volume 42 Issue 3

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miR‑6835‑3p regulates the function of pancreatic islet cells by modulating the expression of AdipoR1

  • Authors:
    • Huimin Wang
    • Lei Jiang
    • Zhenfu Li
    • Wei Wang
    • Chuanji Hao
  • View Affiliations / Copyright

    Affiliations: Department of Critical Care Medicine, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, P.R. China, Department of Cardiac Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1317-1326
    |
    Published online on: June 15, 2018
       https://doi.org/10.3892/ijmm.2018.3731
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Abstract

Effective drugs and strategies for treating type 2 diabetes mellitus (2‑DM) are urgently required. The aim of the present study was to elucidate the mechanism underlying microRNA (miR)‑6835‑3p regulation of adiponectin receptor 1 (AdipoR1) expression and the miR‑6835‑3p/AdipoR1 signaling pathway in pancreatic islet cells. In addition, the potential anti‑diabetes effect of miR‑6835‑3p on insulin secretion was investigated. Luciferase activity analysis was performed to evaluate how miR‑6835‑3p targets the 3'‑untranslated region of AdipoR1. The SU.86.86 and MIN‑6 cell lines were co‑cultured with or without miR‑6835‑3p inhibitors or mimics, and the insulin secretory functions of these cell lines were then determined. Luciferase reporter analysis revealed that AdipoR1 was a direct target of miR‑6835‑3p. In addition, miR‑6835‑3p overexpression suppressed the mRNA and protein expression levels of AdipoR1 in the SU.86.86 and MIN‑6 cell lines. Furthermore, miR‑6835‑3p exerted negative effects on insulin secretion in SU.86.86 and MIN‑6 cells, which were mediated by regulating AdipoR1 expression. AdipoR1 was a direct target of miR‑6835‑3p; therefore, inhibition of AdiopR1 expression may reduce insulin secretion and may be considered a key regulator of insulin secretion. The results of the present study suggested that targeting AdipoR1 with miR‑6835‑3p inhibitors may be a potential strategy for promoting glucose‑stimulated insulin secretion, and thereby, may be an effective treatment for type 2‑DM.
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Copy and paste a formatted citation
Spandidos Publications style
Wang H, Jiang L, Li Z, Wang W and Hao C: miR‑6835‑3p regulates the function of pancreatic islet cells by modulating the expression of AdipoR1. Int J Mol Med 42: 1317-1326, 2018.
APA
Wang, H., Jiang, L., Li, Z., Wang, W., & Hao, C. (2018). miR‑6835‑3p regulates the function of pancreatic islet cells by modulating the expression of AdipoR1. International Journal of Molecular Medicine, 42, 1317-1326. https://doi.org/10.3892/ijmm.2018.3731
MLA
Wang, H., Jiang, L., Li, Z., Wang, W., Hao, C."miR‑6835‑3p regulates the function of pancreatic islet cells by modulating the expression of AdipoR1". International Journal of Molecular Medicine 42.3 (2018): 1317-1326.
Chicago
Wang, H., Jiang, L., Li, Z., Wang, W., Hao, C."miR‑6835‑3p regulates the function of pancreatic islet cells by modulating the expression of AdipoR1". International Journal of Molecular Medicine 42, no. 3 (2018): 1317-1326. https://doi.org/10.3892/ijmm.2018.3731
Copy and paste a formatted citation
x
Spandidos Publications style
Wang H, Jiang L, Li Z, Wang W and Hao C: miR‑6835‑3p regulates the function of pancreatic islet cells by modulating the expression of AdipoR1. Int J Mol Med 42: 1317-1326, 2018.
APA
Wang, H., Jiang, L., Li, Z., Wang, W., & Hao, C. (2018). miR‑6835‑3p regulates the function of pancreatic islet cells by modulating the expression of AdipoR1. International Journal of Molecular Medicine, 42, 1317-1326. https://doi.org/10.3892/ijmm.2018.3731
MLA
Wang, H., Jiang, L., Li, Z., Wang, W., Hao, C."miR‑6835‑3p regulates the function of pancreatic islet cells by modulating the expression of AdipoR1". International Journal of Molecular Medicine 42.3 (2018): 1317-1326.
Chicago
Wang, H., Jiang, L., Li, Z., Wang, W., Hao, C."miR‑6835‑3p regulates the function of pancreatic islet cells by modulating the expression of AdipoR1". International Journal of Molecular Medicine 42, no. 3 (2018): 1317-1326. https://doi.org/10.3892/ijmm.2018.3731
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