Effects of a small, volatile bacterial molecule on Pseudomonas aeruginosa bacteria using whole cell high-resolution magic angle spinning nuclear magnetic resonance spectroscopy and genomics

  • Authors:
    • Valeria Righi
    • Caterina Constantinou
    • Meenu Kesarwani
    • Laurence G. Rahme
    • A. Aria Tzika
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  • Published online on: July 6, 2018     https://doi.org/10.3892/ijmm.2018.3760
  • Pages: 2129-2136
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Abstract

In the present study, high-resolution magic-angle spinning (HRMAS) nuclear magnetic resonance (NMR) spectroscopy was applied to live Pseudomonas aeruginosa (PA) bacterial cells to determine the metabolome of this opportunistic Gram-negative human pathogen, and in particular, its response to the volatile aromatic low molecular weight signaling molecule, 2-aminoacetophenone (2-AA). Multi-dimensional HRMAS NMR is a promising method which may be used to determine the in vivo metabolome of live intact bacterial cells; 2-AA is produced by PA and triggers the emergence of phenotypes that promote chronic infection phenotypes in in vitro and in vivo (animal) models. In the present study, we applied one-dimensional and two-dimensional proton (1H) HRMAS NMR to PA cells which were grown with or without 2-AA in order to examine the associations between metabolites and cellular processes in response to 2-AA. We also compared whole-genome transcriptome profiles of PA cells grown with or without 2-AA and found that 2-AA promoted profound metabolic changes in the PA cells. By comparing the whole-genome transcriptome profiles and metabolomic analysis, we demonstrated that 2-AA profoundly reprogramed the gene expression and metabolic profiles of the cells. Our in vivo 1H HRMAS NMR spectroscopy may prove to be a helpful tool in the validation of gene functions, the study of pathogenic mechanisms, the classification of microbial strains into functional/clinical groups and the testing of anti-bacterial agents.
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October-2018
Volume 42 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Righi V, Constantinou C, Kesarwani M, Rahme LG and Tzika AA: Effects of a small, volatile bacterial molecule on Pseudomonas aeruginosa bacteria using whole cell high-resolution magic angle spinning nuclear magnetic resonance spectroscopy and genomics. Int J Mol Med 42: 2129-2136, 2018
APA
Righi, V., Constantinou, C., Kesarwani, M., Rahme, L.G., & Tzika, A.A. (2018). Effects of a small, volatile bacterial molecule on Pseudomonas aeruginosa bacteria using whole cell high-resolution magic angle spinning nuclear magnetic resonance spectroscopy and genomics. International Journal of Molecular Medicine, 42, 2129-2136. https://doi.org/10.3892/ijmm.2018.3760
MLA
Righi, V., Constantinou, C., Kesarwani, M., Rahme, L. G., Tzika, A. A."Effects of a small, volatile bacterial molecule on Pseudomonas aeruginosa bacteria using whole cell high-resolution magic angle spinning nuclear magnetic resonance spectroscopy and genomics". International Journal of Molecular Medicine 42.4 (2018): 2129-2136.
Chicago
Righi, V., Constantinou, C., Kesarwani, M., Rahme, L. G., Tzika, A. A."Effects of a small, volatile bacterial molecule on Pseudomonas aeruginosa bacteria using whole cell high-resolution magic angle spinning nuclear magnetic resonance spectroscopy and genomics". International Journal of Molecular Medicine 42, no. 4 (2018): 2129-2136. https://doi.org/10.3892/ijmm.2018.3760