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Article

Effects of a small, volatile bacterial molecule on Pseudomonas aeruginosa bacteria using whole cell high-resolution magic angle spinning nuclear magnetic resonance spectroscopy and genomics

  • Authors:
    • Valeria Righi
    • Caterina Constantinou
    • Meenu Kesarwani
    • Laurence G. Rahme
    • A. Aria Tzika
  • View Affiliations / Copyright

    Affiliations: NMR Surgical Laboratory, Center for Surgery, Innovation and Bioengineering, Department of Surgery, Massachusetts General and Shriners Burns Hospitals, Harvard Medical School, Boston, MA 02114, USA, Molecular Surgery Laboratory, Department of Surgery, Microbiology and Immunobiology, Harvard Medical School and Molecular Surgery Laboratory, Center for Surgery, Innovation and Bioengineering, Department of Surgery, Massachusetts General and Shriners Burns Hospitals, Boston, MA 02114, USA
  • Pages: 2129-2136
    |
    Published online on: July 6, 2018
       https://doi.org/10.3892/ijmm.2018.3760
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Abstract

In the present study, high-resolution magic-angle spinning (HRMAS) nuclear magnetic resonance (NMR) spectroscopy was applied to live Pseudomonas aeruginosa (PA) bacterial cells to determine the metabolome of this opportunistic Gram-negative human pathogen, and in particular, its response to the volatile aromatic low molecular weight signaling molecule, 2-aminoacetophenone (2-AA). Multi-dimensional HRMAS NMR is a promising method which may be used to determine the in vivo metabolome of live intact bacterial cells; 2-AA is produced by PA and triggers the emergence of phenotypes that promote chronic infection phenotypes in in vitro and in vivo (animal) models. In the present study, we applied one-dimensional and two-dimensional proton (1H) HRMAS NMR to PA cells which were grown with or without 2-AA in order to examine the associations between metabolites and cellular processes in response to 2-AA. We also compared whole-genome transcriptome profiles of PA cells grown with or without 2-AA and found that 2-AA promoted profound metabolic changes in the PA cells. By comparing the whole-genome transcriptome profiles and metabolomic analysis, we demonstrated that 2-AA profoundly reprogramed the gene expression and metabolic profiles of the cells. Our in vivo 1H HRMAS NMR spectroscopy may prove to be a helpful tool in the validation of gene functions, the study of pathogenic mechanisms, the classification of microbial strains into functional/clinical groups and the testing of anti-bacterial agents.
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Copy and paste a formatted citation
Spandidos Publications style
Righi V, Constantinou C, Kesarwani M, Rahme LG and Tzika AA: Effects of a small, volatile bacterial molecule on Pseudomonas aeruginosa bacteria using whole cell high-resolution magic angle spinning nuclear magnetic resonance spectroscopy and genomics. Int J Mol Med 42: 2129-2136, 2018.
APA
Righi, V., Constantinou, C., Kesarwani, M., Rahme, L.G., & Tzika, A.A. (2018). Effects of a small, volatile bacterial molecule on Pseudomonas aeruginosa bacteria using whole cell high-resolution magic angle spinning nuclear magnetic resonance spectroscopy and genomics. International Journal of Molecular Medicine, 42, 2129-2136. https://doi.org/10.3892/ijmm.2018.3760
MLA
Righi, V., Constantinou, C., Kesarwani, M., Rahme, L. G., Tzika, A. A."Effects of a small, volatile bacterial molecule on Pseudomonas aeruginosa bacteria using whole cell high-resolution magic angle spinning nuclear magnetic resonance spectroscopy and genomics". International Journal of Molecular Medicine 42.4 (2018): 2129-2136.
Chicago
Righi, V., Constantinou, C., Kesarwani, M., Rahme, L. G., Tzika, A. A."Effects of a small, volatile bacterial molecule on Pseudomonas aeruginosa bacteria using whole cell high-resolution magic angle spinning nuclear magnetic resonance spectroscopy and genomics". International Journal of Molecular Medicine 42, no. 4 (2018): 2129-2136. https://doi.org/10.3892/ijmm.2018.3760
Copy and paste a formatted citation
x
Spandidos Publications style
Righi V, Constantinou C, Kesarwani M, Rahme LG and Tzika AA: Effects of a small, volatile bacterial molecule on Pseudomonas aeruginosa bacteria using whole cell high-resolution magic angle spinning nuclear magnetic resonance spectroscopy and genomics. Int J Mol Med 42: 2129-2136, 2018.
APA
Righi, V., Constantinou, C., Kesarwani, M., Rahme, L.G., & Tzika, A.A. (2018). Effects of a small, volatile bacterial molecule on Pseudomonas aeruginosa bacteria using whole cell high-resolution magic angle spinning nuclear magnetic resonance spectroscopy and genomics. International Journal of Molecular Medicine, 42, 2129-2136. https://doi.org/10.3892/ijmm.2018.3760
MLA
Righi, V., Constantinou, C., Kesarwani, M., Rahme, L. G., Tzika, A. A."Effects of a small, volatile bacterial molecule on Pseudomonas aeruginosa bacteria using whole cell high-resolution magic angle spinning nuclear magnetic resonance spectroscopy and genomics". International Journal of Molecular Medicine 42.4 (2018): 2129-2136.
Chicago
Righi, V., Constantinou, C., Kesarwani, M., Rahme, L. G., Tzika, A. A."Effects of a small, volatile bacterial molecule on Pseudomonas aeruginosa bacteria using whole cell high-resolution magic angle spinning nuclear magnetic resonance spectroscopy and genomics". International Journal of Molecular Medicine 42, no. 4 (2018): 2129-2136. https://doi.org/10.3892/ijmm.2018.3760
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