Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
December-2018 Volume 42 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2018 Volume 42 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Inflammatory stimuli promote oxidative stress in pancreatic acinar cells via Toll-like receptor 4/nuclear factor-κB pathway

  • Authors:
    • Longfei Pan
    • Lei Yu
    • Liming Wang
    • Juntao He
    • Jiangli Sun
    • Xiaobo Wang
    • Hai Wang
    • Zhenghai Bai
    • Hui Feng
    • Honghong Pei
  • View Affiliations / Copyright

    Affiliations: Department of Emergency Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China, Department of Basic Medicine, Xi'an Medical College, Xi'an, Shaanxi 710021, P.R. China, Clinical Laboratory, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China
  • Pages: 3582-3590
    |
    Published online on: October 1, 2018
       https://doi.org/10.3892/ijmm.2018.3906
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The Toll‑like receptor 4/nuclear factor‑κB (TLR4/NF‑κB) pathway is vital to the pathogenesis of acute pancreatitis (AP). The aim of the present study was to identify the mechanism of the activation of the TLR4/NF‑κB signaling pathway in the viability of primary pancreatic cells. The cells were stimulated with lipopolysaccharide (LPS) for the activation of NF‑κB signaling. Next, the reactive oxygen species (ROS) level was evaluated by detecting the concentration of malondialdehyde and glutathione peroxidase. Cell viability was measured by Cell Counting Kit‑8 and MTT assays, while the percentage of apoptosis was detected by flow cytometry. Quantitative polymerase chain reaction was used to detect TLR4, B‑cell lymphoma 2 (Bcl2), Bcl2‑associated X protein (Bax) and phorbol‑12‑myristate‑13‑acetate‑induced protein 1 (PMAIP1) expression levels. Western blot assay was also conducted to detect TLR4 protein expression, while the activity of NF‑κB signaling was measured by determining the p65 and phosphorylated p65 protein levels. In addition, the effect of TLR4 overexpression or treatment with TLR4 antagonists in the presence of LPS stimulation was investigated. The results revealed that ROS levels were increased and cell viability was decreased in LPS‑stimulated pancreatic acinar cells. TLR4, Bax and PMAIP1 levels were increased, Bcl2 expression was decreased and NF‑κB signaling was activated in LPS‑stimulated pancreatic acinar cells. Furthermore, pancreatic cells with TLR4 overexpression exhibited increased ROS level and decreased viability. Finally, the effect caused by LPS stimulation was partially reversed by treatment of pancreatic acinar cells with TLR4 antagonists. In conclusion, the current study investigated a novel regulatory mechanism of the TLR4/NF‑κB pathway in LPS‑stimulated pancreatic cells, which may contribute to pancreatitis. The damage of these cells due to increased ROS levels was observed to occur through activation of the TLR4/NF‑κB pathway.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

LaRusch J, Solomon S and Whitcomb DC: Pancreatitis Overview. GeneReviews(R). Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Ledbetter N, Mefford HC, Smith RJH, et al: University of Washington; Seattle, WA: 1993

2 

Charbonney E and Nathens AB: Severe acute pancreatitis: A review. Surg Infect (Larchmt). 9:573–578. 2008. View Article : Google Scholar

3 

Kawagishi H and Finkel T: Unraveling the truth about antioxidants: ROS and disease: Finding the right balance. Nat Med. 20:711–713. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Shi C, Andersson R, Zhao X and Wang X: Potential role of reactive oxygen species in pancreatitis-associated multiple organ dysfunction. Pancreatology. 5:492–500. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Booth DM, Mukherjee R, Sutton R and Criddle DN: Calcium and reactive oxygen species in acute pancreatitis: Friend or foe? Antioxid Redox Signal. 15:2683–2698. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Simon HU, Haj-Yehia A and Levi-Schaffer F: Role of reactive oxygen species (ROS) in apoptosis induction. Apoptosis. 5:415–418. 2000. View Article : Google Scholar

7 

Park J, Min JS, Kim B, Chae UB, Yun JW, Choi MS, Kong IK, Chang KT and Lee DS: Mitochondrial ROS govern the LPS-induced pro-inflammatory response in microglia cells by regulating MAPK and NF-κB pathways. Neurosci Lett. 584:191–196. 2015. View Article : Google Scholar

8 

Cho RL, Yang CC, Lee IT, Lin CC, Chi PL, Hsiao LD and Yang CM: Lipopolysaccharide induces ICAM-1 expression via a c-Src/NADPH oxidase/ROS-dependent NF-κB pathway in human pulmonary alveolar epithelial cells. Am J Physiol Lung Cell Mol Physiol. 310:L639–L657. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Lv JC, Wang G, Pan SH, Bai XW and Sun B: Lycopene protects pancreatic acinar cells against severe acute pancreatitis by abating the oxidative stress through JNK pathway. Free Radic Res. 49:151–163. 2015. View Article : Google Scholar

10 

Ramudo L, Manso MA, Sevillano S and de Dios I: Kinetic study of TNF-alpha production and its regulatory mechanisms in acinar cells during acute pancreatitis induced by bile-pancreatic duct obstruction. J Pathol. 206:9–16. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Keck T, Werner J, Banafsche R, Stalmann A, Schneider L, Gebhard MM, Herfarth C and Klar E: Oxygen radicals promote ICAM-1 expression and microcirculatory disturbances in experimental acute pancreatitis. Pancreatology. 3:156–163. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Song R, Yu D and Park J: Changes in gene expression of tumor necrosis factor alpha and interleukin 6 in a canine model of caerulein-induced pancreatitis. Can J Vet Res. 80:236–241. 2016.PubMed/NCBI

13 

Concepcion-Martin M, Gomez-Oliva C, Juanes A, Mora J, Vidal S, Diez X, Torras X, Sainz S, Villanueva C, Farre A, et al: IL-6, IL-10 and TNFα do not improve early detection of post-endoscopic retrograde cholangiopancreatography acute pancreatitis: A prospective cohort study. Sci Rep. 6:334922016. View Article : Google Scholar

14 

Yang ZW, Meng XX, Zhang C and Xu P: CARD9 gene silencing with siRNA protects rats against severe acute pancreatitis: CARD9-dependent NF-κB and P38MAPKs pathway. J Cell Mol Med. 21:1085–1093. 2017. View Article : Google Scholar

15 

Wang WY, Chen Y, Su X, Tang D, Ben QW, Yao WY, Chen P and Yuan YZ: Resistin-like molecule-α causes lung injury in rats with acute pancreatitis by activating the pi-3k/akt-NF-κB pathway and promoting inflammatory cytokine release. Curr Mol Med. 16:677–687. 2016. View Article : Google Scholar

16 

Jiang CY and Wang W: Resistin aggravates the expression of proinflammatory cytokines in ceruleinstimulated AR42J pancreatic acinar cells. Mol Med Rep. 15:502–506. 2017. View Article : Google Scholar

17 

DiDonato JA, Mercurio F and Karin M: NF-κB and the link between inflammation and cancer. Immunol Rev. 246:379–400. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Gukovsky I, Gukovskaya AS, Blinman TA, Zaninovic V and Pandol SJ: Early NF-kappaB activation is associated with hormone-induced pancreatitis. Am J Physiol. 275:G1402–G1414. 1998.PubMed/NCBI

19 

Huang H, Liu Y, Daniluk J, Gaiser S, Chu J, Wang H, Li ZS, Logsdon CD and Ji B: Activation of nuclear factor-κB in acinar cells increases the severity of pancreatitis in mice. Gastroenterology. 144:202–210. 2013. View Article : Google Scholar

20 

Liu Z, Liu J, Zhao K, Shi Q, Zuo T, Wang G and Wang W: Role of daphnetin in rat severe acute pancreatitis through the regulation of TLR4/NF-[formula: See text]B signaling pathway activation. Am J Chin Med. 44:149–163. 2016. View Article : Google Scholar

21 

Cen Y, Liu C, Li X, Yan Z, Kuang M, Su Y, Pan X, Qin R, Liu X, Zheng J and Zhou H: Artesunate ameliorates severe acute pancre-atitis (SAP) in rats by inhibiting expression of pro-inflammatory cytokines and Toll-like receptor 4. Int Immunopharmacol. 38:252–260. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Pan LF, Yu L, Wang LM, He JT, Sun JL, Wang XB, Bai ZH, Su LJ and Pei HH: The toll-like receptor 4 antagonist transforming growth factor-β-activated kinase(TAK)-242 attenuates taurocholate-induced oxidative stress through regulating mitochondrial function in mice pancreatic acinar cells. J Surg Res. 206:298–306. 2016. View Article : Google Scholar : PubMed/NCBI

23 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

24 

Li X, Zhang L, Xu YW, Wang C, Zhao Y, Yu P, Lv SW, Yan GL, Liu JQ and Luo GM: The protective effects of 6-CySeCD with GPx activity against UVB-induced injury in HaCaT cells. Australas J Dermatol. 54:120–125. 2013. View Article : Google Scholar

25 

Zhang X, Zhu C, Wu D and Jiang X: Possible role of toll-like receptor 4 in acute pancreatitis. Pancreas. 39:819–824. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Jimenez-Dalmaroni MJ, Gerswhin ME and Adamopoulos IE: The critical role of toll-like receptors-from microbial recognition to autoimmunity: A comprehensive review. Autoimmun Rev. 15:1–8. 2016. View Article : Google Scholar

27 

Joosten LA, Abdollahi-Roodsaz S, Dinarello CA, O'Neill L and Netea MG: Toll-like receptors and chronic inflammation in rheumatic diseases: New developments. Nat Rev Rheumatol. 12:344–357. 2016. View Article : Google Scholar : PubMed/NCBI

28 

Zhou Y, Jin XH, Jing YX, Song Y, He XX, Zheng LL, Wang YB, Wei ZY and Zhang GP: Porcine parvovirus infection activates inflammatory cytokine production through Toll-like receptor 9 and NF-κB signaling pathways in porcine kidney cells. Vet Microbiol. 207:56–62. 2017. View Article : Google Scholar : PubMed/NCBI

29 

Lunin SM, Khrenov MO, Novoselova TV, Parfenyuk SB, Glushkova OV, Fesenko EE and Novoselova EG: Modulation of inflammatory response in mice with severe autoimmune disease by thymic peptide thymulin and an inhibitor of NF-kappaB signalling. Int Immunopharmacol. 25:260–266. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Bhaskar S, Sudhakaran PR and Helen A: Quercetin attenuates atherosclerotic inflammation and adhesion molecule expression by modulating TLR-NF-κB signaling pathway. Cell Immunol. 310:131–140. 2016. View Article : Google Scholar : PubMed/NCBI

31 

Wu LY, Ye ZN, Zhou CH, Wang CX, Xie GB, Zhang XS, Gao YY, Zhang ZH, Zhou ML, Zhuang Z, et al: Roles of pannexin-1 channels in inflammatory response through the TLRs/NF-Kappa B signaling pathway following experimental subarachnoid hemorrhage in rats. Front Mol Neurosci. 10:1752017. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Pan L, Yu L, Wang L, He J, Sun J, Wang X, Wang H, Bai Z, Feng H, Pei H, Pei H, et al: Inflammatory stimuli promote oxidative stress in pancreatic acinar cells via Toll-like receptor 4/nuclear factor-κB pathway. Int J Mol Med 42: 3582-3590, 2018.
APA
Pan, L., Yu, L., Wang, L., He, J., Sun, J., Wang, X. ... Pei, H. (2018). Inflammatory stimuli promote oxidative stress in pancreatic acinar cells via Toll-like receptor 4/nuclear factor-κB pathway. International Journal of Molecular Medicine, 42, 3582-3590. https://doi.org/10.3892/ijmm.2018.3906
MLA
Pan, L., Yu, L., Wang, L., He, J., Sun, J., Wang, X., Wang, H., Bai, Z., Feng, H., Pei, H."Inflammatory stimuli promote oxidative stress in pancreatic acinar cells via Toll-like receptor 4/nuclear factor-κB pathway". International Journal of Molecular Medicine 42.6 (2018): 3582-3590.
Chicago
Pan, L., Yu, L., Wang, L., He, J., Sun, J., Wang, X., Wang, H., Bai, Z., Feng, H., Pei, H."Inflammatory stimuli promote oxidative stress in pancreatic acinar cells via Toll-like receptor 4/nuclear factor-κB pathway". International Journal of Molecular Medicine 42, no. 6 (2018): 3582-3590. https://doi.org/10.3892/ijmm.2018.3906
Copy and paste a formatted citation
x
Spandidos Publications style
Pan L, Yu L, Wang L, He J, Sun J, Wang X, Wang H, Bai Z, Feng H, Pei H, Pei H, et al: Inflammatory stimuli promote oxidative stress in pancreatic acinar cells via Toll-like receptor 4/nuclear factor-κB pathway. Int J Mol Med 42: 3582-3590, 2018.
APA
Pan, L., Yu, L., Wang, L., He, J., Sun, J., Wang, X. ... Pei, H. (2018). Inflammatory stimuli promote oxidative stress in pancreatic acinar cells via Toll-like receptor 4/nuclear factor-κB pathway. International Journal of Molecular Medicine, 42, 3582-3590. https://doi.org/10.3892/ijmm.2018.3906
MLA
Pan, L., Yu, L., Wang, L., He, J., Sun, J., Wang, X., Wang, H., Bai, Z., Feng, H., Pei, H."Inflammatory stimuli promote oxidative stress in pancreatic acinar cells via Toll-like receptor 4/nuclear factor-κB pathway". International Journal of Molecular Medicine 42.6 (2018): 3582-3590.
Chicago
Pan, L., Yu, L., Wang, L., He, J., Sun, J., Wang, X., Wang, H., Bai, Z., Feng, H., Pei, H."Inflammatory stimuli promote oxidative stress in pancreatic acinar cells via Toll-like receptor 4/nuclear factor-κB pathway". International Journal of Molecular Medicine 42, no. 6 (2018): 3582-3590. https://doi.org/10.3892/ijmm.2018.3906
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team