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Article Open Access

In vitro analysis of microRNA-26a in chronic lymphocytic leukemia cells

  • Authors:
    • Jing Li
    • Chang‑Kui Sun
  • View Affiliations / Copyright

    Affiliations: Blood Group Reference Laboratory, Shandong Blood Center, Jinan, Shandong 250014, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3364-3370
    |
    Published online on: October 10, 2018
       https://doi.org/10.3892/ijmm.2018.3925
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Abstract

microRNA (miRNA)‑26a‑loaded liposomes were prepared in the present study for effective treatment of leukemia. The results demonstrated that miRNA‑26a reduced the viability of chronic lymphocytic leukemia (CLL) cells in a concentration‑dependent manner. Cells treated with miRNA‑26a‑loaded liposomes exhibited increased rates of apoptosis, as determined by flow cytometry and Hoechst 33342 staining. Western blot analysis revealed an increased apoptotic effect of miRNA‑26a‑loaded liposomes compared with control. Treatment with these liposomes resulted in significant downregulation of the expression of the miRNA‑26a target genes, myeloid cell leukemia 1 and cyclin‑dependent kinase 6. Taken together, the results of the present study indicate that miRNA‑26a exerts apoptosis‑inducing and anticancer effects on leukemia cells, suggesting therapeutic potential. This approach may be possible to extrapolate to other neoplasms, including lymphomas and acute myeloid leukemia.
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Copy and paste a formatted citation
Spandidos Publications style
Li J and Sun CK: In vitro analysis of microRNA-26a in chronic lymphocytic leukemia cells. Int J Mol Med 42: 3364-3370, 2018.
APA
Li, J., & Sun, C. (2018). In vitro analysis of microRNA-26a in chronic lymphocytic leukemia cells. International Journal of Molecular Medicine, 42, 3364-3370. https://doi.org/10.3892/ijmm.2018.3925
MLA
Li, J., Sun, C."In vitro analysis of microRNA-26a in chronic lymphocytic leukemia cells". International Journal of Molecular Medicine 42.6 (2018): 3364-3370.
Chicago
Li, J., Sun, C."In vitro analysis of microRNA-26a in chronic lymphocytic leukemia cells". International Journal of Molecular Medicine 42, no. 6 (2018): 3364-3370. https://doi.org/10.3892/ijmm.2018.3925
Copy and paste a formatted citation
x
Spandidos Publications style
Li J and Sun CK: In vitro analysis of microRNA-26a in chronic lymphocytic leukemia cells. Int J Mol Med 42: 3364-3370, 2018.
APA
Li, J., & Sun, C. (2018). In vitro analysis of microRNA-26a in chronic lymphocytic leukemia cells. International Journal of Molecular Medicine, 42, 3364-3370. https://doi.org/10.3892/ijmm.2018.3925
MLA
Li, J., Sun, C."In vitro analysis of microRNA-26a in chronic lymphocytic leukemia cells". International Journal of Molecular Medicine 42.6 (2018): 3364-3370.
Chicago
Li, J., Sun, C."In vitro analysis of microRNA-26a in chronic lymphocytic leukemia cells". International Journal of Molecular Medicine 42, no. 6 (2018): 3364-3370. https://doi.org/10.3892/ijmm.2018.3925
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