Suppressive effect mediated by human adipose-derived stem cells on T cells involves the activation of JNK

Wang,Yinmin; Wang,Xiuxia; Zhou,Xianyu; Zhu,Zhu; Yang,Jun; Liu,Fei

doi:10.3892/ijmm.2018.3953

Suppressive effect mediated by human adipose-derived stem cells on T cells involves the activation of JNK

Authors:
- Yinmin Wang
- Xiuxia Wang
- Xianyu Zhou
- Zhu Zhu
- Jun Yang
- Fei Liu
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Affiliations: Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China
Published online on: October 24, 2018 https://doi.org/10.3892/ijmm.2018.3953
Pages: 177-184
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Adipose‑derived stem cells (ADSCs) have an immunomodulatory role in vascularized composite tissue allo‑transplantation (VCA). However, the specific effects of ADSCs on lymphocytes remain to be fully elucidated. The present study examined the changes in T cells co‑cultured with ADSCs in terms of the proliferation by Cell Counting Kit‑8 assay, cell cycle profile and apoptosis by flow cytometry, inflammatory cytokine production by polymerase chain reaction and ELISA, in addition to the expression of survival proteins by western blotting. The ADSCs reduced the viability of Jurkat T cells and downregulated the transcription of tumor necrosis factor‑α and transforming growth factor‑β1. Co‑culture with ADSCs also induced apoptosis and increased the levels of phosphorylated c‑Jun N‑terminal kinase in the T cells. Taken together, these findings confirmed that ADSCs modulate the host immune response by suppressing T cells.

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