Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
January-2019 Volume 43 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2019 Volume 43 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

SENP1 has an important role in lung development and influences the differentiation of alveolar type 2 cells

  • Authors:
    • Xue‑Qing Wan
    • Jia‑Yu Cai
    • Yue Zhu
    • Qiu‑Xia Wang
    • Hai‑Tao Zhu
    • Hui‑Min Ju
    • Hong‑Yan Lu
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212000, P.R. China
    Copyright: © Wan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 371-381
    |
    Published online on: October 29, 2018
       https://doi.org/10.3892/ijmm.2018.3964
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Post‑translational modification via small ubiquitin‑like modifier (SUMO) is involved in the regulation of various important cellular processes. SUMO modification can be regulated at the level of conjugation, and can also be reversed by the SUMO‑specific proteases (SENPs). However, current studies of the regulation and function of SENP in lung development remain limited. In this study, the expression levels of SENP1 and SUMO1 were assessed during lung development in rats. SUMO1 modification occurred during lung development and changes in SENP1 expression were consistent with the changes in the presence of free SUMO1. In order to investigate the function of SENP1, alveolar type (AT) 2 cells were transfected with SENP1‑targeting small interfering RNA, and the proliferation, apoptosis and differentiation function of AT2 cells was subsequently evaluated. Marked upregulation of conjugated SUMO1 was observed following SENP1 inhibition. Furthermore, depletion of SENP1 resulted in increased apoptosis, decreased proliferation and impaired differentiation status of AT2 cells. Thus, the results support that SENP1 is an essential regulator of the balance between SUMOylation and deSUMOylation during lung development, specifically affecting the proliferation and differentiation status of AT2 cells.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

Figure 10

View References

1 

Warburton D and Bellusci S: The molecular genetics of lung morphogenesis and injury repair. Paediatr Respir Rev. 5(Suppl A): S283–S287. 2004. View Article : Google Scholar : PubMed/NCBI

2 

Claudio N and Morty RE: MicroRNA in late lung development and bronchopulmonary dysplasia: The need to demonstrate causality. Mol Cell Pediatr. 3:192016. View Article : Google Scholar

3 

Barkauskas CE, Cronce MJ, Rackley CR, Bowie EJ, Keene DR, Stripp BR, Randell SH, Noble PW and Hogan LM: Type 2 alveolar cells are stem cells in adult lung. J Clin Invest. 123:3025–3036. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Adamson IY and Bowden DH: The type 2 cell as progenitor of alveolar epithelial regeneration: A cytodynamic study in mice after exposure to oxygen. Lab Invest. 30:35–42. 1974.PubMed/NCBI

5 

Adamson IY and Bowden DH: Derivation of type 1 epithelium from type 2 cells in the developing rat lung. Lab Invest. 32:736–745. 1975.PubMed/NCBI

6 

Siegel R, Naishadham D and Jemal A: Cancer statistics. CA Cancer J Clin. 63:11–30. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Lomelí H and Vázquez M: Emerging roles of the SUMO pathway in development. Cell Mol Life Sci. 68:4045–4064. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Garciadominguez M and Reyes JC: SUMO association with repressor complexes, emerging routes for transcriptional control. Biochim Biophys Acta. 1789:451–459. 2009. View Article : Google Scholar

9 

Tempé D, Piechaczyk M and Bossis G: SUMO under stress. Biochem Soc Trans. 36:874–878. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Kim JH and Baek SH: Emerging roles of desumoylating enzymes. Biochim Biophys Acta. 1792:155–162. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Saho E, Takuya A, Hiroshi A, Shunsuke K, Barnabas S, Yusuke Y, Akira M, Shunichi T and Dana B: The SUMO protease SENP1 is required for cohesion maintenance and mitotic arrest following spindle poison treatment. Biochem Biophys Res Commun. 42:310–316. 2012.

12 

Chen CH, Chang CC, Lee TH, Luo M, Huang P, Liao P, Wei S, Li F, Chen R, Zhou XZ, et al: SENP1 deSUMOylates and regulates Pin1 protein activity and cellular function. Cancer Res. 73:3951–3962. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Flotho A and Melchior F: Sumoylation: A regulatory protein modification in health and disease. Annu Rev Biochem. 82:357–385. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Bawa-Khalfe T and Yeh ET: SUMO losing balance: SUMO proteases disrupt SUMO homeostasis to facilitate cancer development and progression. Genes Cancer. 1:748–752. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Nacerddine K, Lehembre F, Bhaumik M, Artus J, Cohen- Tannoudji M, Babinet C, Pandolfi PP and Dejean A: The SUMO pathway is essential for nuclear integrity and chromosome segregation in mice. Dev Cell. 9:769–779. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Yamaguchi T, Sharma P, Athanasiou M, Kumar A, Yamada S and Kuehn MR: Mutation of SENP1/SuPr-2 reveals an essential role for desumoylation in mouse development. Mol Cell Biol. 25:5171–5182. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Chen YD, Liu JY, Lu YM, Zhu HT, Tang W, Wang QX and Lu HY: Functional roles of C/EBPα and SUMO-modification in lung development. Int J Mol Med. 40:1037–1046. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Zhou F, Dai A, Fu D, Jiang Y, Tan X and Zhang X: SENP-1 enhances hypoxia-induced proliferation of rat pulmonary artery smooth muscle cells by regulating hypoxia-inducible factor-1α. Mol Med Rep. 13:3482–3490. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Jiang Y, Wang J, Tian H, Li G, Zhu H, Liu L, Hu R and Dai A: Increased SUMO-1 expression in response to hypoxia: Interaction with HIF-1α in hypoxic pulmonary hypertension. Int J Mol Med. 36:271–281. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Pandey D, Nomura Y, Rossberg MC, Hori D, Bhatta A, Keceli G, Leucker T, Santhanam L, Shimoda LA, Berkowitz D and Romer L: Hypoxia triggers SENP1 (sentrin-specific protease 1) modulation of KLF15 (Kruppel-like factor 15) and transcriptional regulation of Arg2 (Arginase 2) in pulmonary endothelium. Arterioscler Thromb Vasc Biol. 38:913–926. 2018. View Article : Google Scholar : PubMed/NCBI

21 

Wang RT, Zhi XY, Zhang Y and Zhang J: Inhibition of SENP1 induces radiosensitization in lung cancer cells. Exp Ther Med. 6:1054–1058. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

23 

Gao RW, Kong XY, Zhu XX, Zhu GQ, Ma JS and Liu XX: Retinoic acid promotes primary fetal alveolar epithelial type II cell proliferation and differentiation to alveolar epithelial type I cells. In Vitro Cell Dev Biol Anim. 51:479–487. 2015. View Article : Google Scholar

24 

Sharma P, Yamada S, Lualdi M, Dasso M and Kuehn MR: SENP1 is essential for desumoylating SUMO1-modified proteins but dispensable for SUMO2 and SUMO3 deconjugation in the mouse embryo. Cell Rep. 3:1640–1650. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Juarez-Vicente F, Luna-Pelaez N and Garcia-Dominguez M: The SUMO protease SENP7 is required for proper neuronal differentiation. Biochim Biophys Acta. 1863:1490–1498. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Besnard V, Nabeyrat E, Henrion-Caude A, Chadelat K, Perin L, Le Boucn Y and Clement A: Protective role of retinoic acid from antiproliferative action of TNF-alpha on lung epithelial cells. Am J Physiol Lung Cell Mol Physiol. 282:L863–L871. 2002. View Article : Google Scholar : PubMed/NCBI

27 

Londhe VA, Maisonet TM, Lopez B, Shin BC, Huynh J and Devaskar SU: Retinoic acid rescues alveolar hypoplasia in the calorierestricted developing rat lung. Am J Respir Cell Mol Biol. 48:179–187. 2013. View Article : Google Scholar :

28 

Evans MJ, Cabral LJ, Stephens RJ and Freeman G: Renewal of alveolar epithelium in the rat following exposure to NO2. Am J Pathol. 70:175–198. 1973.PubMed/NCBI

29 

Lu H, Chang L, Li W, Jiang N, Peng Q, Cai C and Liu J: Effects of hyperoxia on the dynamic expression of Aquaporin5 in premature rats lung development. J Huazhong Univ Sci Technolog Med Sci. 27:318–312. 2007. View Article : Google Scholar : PubMed/NCBI

30 

George UM, Ashna U, Kumar SS and Nandkumar AM: Effect of tobacco extract on surfactant synthesis and its reversal by retinoic acid-role of cell-cell interactions in vitro. In Vitro Cell Dev Biol Anim. 49:260–269. 2013. View Article : Google Scholar : PubMed/NCBI

31 

Nomura J, Horie I, Seto M, Nagai K, Hisatsune A, Miyata T and Isohama Y: All-trans retinoic acid increases expression of aquaporin-5 and plasma membrane water permeability via transactivation of Sp1 in mouse lung epithelial cells. Biochem Biophys Res Commun. 351:1048–1053. 2006. View Article : Google Scholar : PubMed/NCBI

32 

Schittny JC: Development of the lung. Cell Tissue Res. 367:427–444. 2017. View Article : Google Scholar : PubMed/NCBI

33 

Yuasa E and Saitoh H: In situ SUMOylation and DeSUMOylation assays: fluorescent methods to visualize SUMOylation and DeSUMOylation in permeabilized cells. Methods Mol Biol. 1475:151–159. 2016. View Article : Google Scholar : PubMed/NCBI

34 

Desai TJ, Brownfield DG and Krasnow MA: Alveolar progenitor and stem cells in lung development, renewal and cancer. Nature. 507:190–194. 2014. View Article : Google Scholar : PubMed/NCBI

35 

Tan F, Dong W, Lei X, Li Q, Kang L, Zhao S and Zhang C: Attenuated SUMOylation of sirtuin 1 in premature neonates with bronchopulmonary dysplasia. Mol Med Rep. 17:1283–1288. 2018.

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wan XQ, Cai JY, Zhu Y, Wang QX, Zhu HT, Ju HM and Lu HY: SENP1 has an important role in lung development and influences the differentiation of alveolar type 2 cells. Int J Mol Med 43: 371-381, 2019.
APA
Wan, X., Cai, J., Zhu, Y., Wang, Q., Zhu, H., Ju, H., & Lu, H. (2019). SENP1 has an important role in lung development and influences the differentiation of alveolar type 2 cells. International Journal of Molecular Medicine, 43, 371-381. https://doi.org/10.3892/ijmm.2018.3964
MLA
Wan, X., Cai, J., Zhu, Y., Wang, Q., Zhu, H., Ju, H., Lu, H."SENP1 has an important role in lung development and influences the differentiation of alveolar type 2 cells". International Journal of Molecular Medicine 43.1 (2019): 371-381.
Chicago
Wan, X., Cai, J., Zhu, Y., Wang, Q., Zhu, H., Ju, H., Lu, H."SENP1 has an important role in lung development and influences the differentiation of alveolar type 2 cells". International Journal of Molecular Medicine 43, no. 1 (2019): 371-381. https://doi.org/10.3892/ijmm.2018.3964
Copy and paste a formatted citation
x
Spandidos Publications style
Wan XQ, Cai JY, Zhu Y, Wang QX, Zhu HT, Ju HM and Lu HY: SENP1 has an important role in lung development and influences the differentiation of alveolar type 2 cells. Int J Mol Med 43: 371-381, 2019.
APA
Wan, X., Cai, J., Zhu, Y., Wang, Q., Zhu, H., Ju, H., & Lu, H. (2019). SENP1 has an important role in lung development and influences the differentiation of alveolar type 2 cells. International Journal of Molecular Medicine, 43, 371-381. https://doi.org/10.3892/ijmm.2018.3964
MLA
Wan, X., Cai, J., Zhu, Y., Wang, Q., Zhu, H., Ju, H., Lu, H."SENP1 has an important role in lung development and influences the differentiation of alveolar type 2 cells". International Journal of Molecular Medicine 43.1 (2019): 371-381.
Chicago
Wan, X., Cai, J., Zhu, Y., Wang, Q., Zhu, H., Ju, H., Lu, H."SENP1 has an important role in lung development and influences the differentiation of alveolar type 2 cells". International Journal of Molecular Medicine 43, no. 1 (2019): 371-381. https://doi.org/10.3892/ijmm.2018.3964
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team