C1QTNF6 is overexpressed in gastric carcinoma and contributes to the proliferation and migration of gastric carcinoma cells

  • Authors:
    • Hai‑Xia Qu
    • Lin Cui
    • Xin‑Ying Meng
    • Zhi‑Jie Wang
    • Yan‑Xin Cui
    • Yun‑Peng Yu
    • Dong Wang
    • Xiang‑Jun Jiang
  • View Affiliations

  • Published online on: November 6, 2018     https://doi.org/10.3892/ijmm.2018.3978
  • Pages: 621-629
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Abstract

In the present study, proteins differentially expressed between gastric cancer tissue and para‑tumoral normal gastric tissues were screened, and the function of the highly expressed protein C1QTNF6 in gastric carcinoma was investigated. The differential expression of mRNAs extracted from the tumor and adjacent tissues was analyzed using GeneChip assay. An AGS si‑C1QTNF6 cell line was constructed using shRNA‑C1QTNF6 lentivirus. The cell invasion and migration ability of C1QTNF6‑knockdown cells were determined by Transwell chamber migration and wound healing assays, respectively. The effects of C1QTNF6 on AGS cell cycle distribution and apoptosis were detected using a FACScan flow cytometer. The results demonstrated that the expression of 109 genes was increased and the expression of 129 was decreased in tumor tissues. Among these genes, the C1QTNF6 gene was highly expressed in tumor tissues and the AGS7901 cell line. C1QTNF6‑knockdown decreased the cell growth, and the proliferative and migration ability, as well as increasing the apoptosis of gastric carcinoma cells. In addition, the number of AGS cells in the G2/M phase was significantly increased after 5 days of C1QTNF6‑shRNA lentivirus infection. The results of the present study indicated that C1QTNF6 serves an important role in the development of gastric carcinoma. C1QTNF6 is involved in promoting the proliferation and migration, and in reducing the apoptosis of gastric carcinoma cells. These results provided a potential therapeutic target for the treatment of gastric carcinoma.

Introduction

Gastric carcinoma (GC) is the fourth most prevalent type of cancer and is the second highest contributor to global cancer-related mortality (1). GC remains the second most common cause of cancer-associated mortality and health problems in China, despite the lower incidence of GC observed over the past several years (2). Although gastric cancer can be surgically excised and inhibited by chemotherapy, gastric tumor cells continue to grow and metastasize elsewhere in the body, which leads to a poor prognosis. Uncontrolled tumor cell proliferation and migration are two important features of GC. Therefore, inhibition of cancer cell proliferation and migration, and promotion of cancer cell apoptosis are promising therapeutic targets for GC. Investigation of the pathological characteristics of GC and associated mechanisms will provide novel insights for the discovery of potential therapeutic targets.

C1q and tumor necrosis factor superfamily are involved in several biological processes, including inflammation, apoptosis and cell differentiation. C1q/tumor necrosis factor-related proteins (CTRP proteins) have been revealed to serve a role in carcinogenesis and cancer progression (3). The C1q/TNF-related protein family is comprised of 16 CTRP members, CTRP1-9, 9B, 10-15. Among these, CTRP3, CTRP4 and C1QTNF6 have been revealed to be associated with tumor promotion. All CTRP members are secreted proteins, and are widely expressed in various tissues and cell types (4-8). CTRP4 was demonstrated to function as a tumor-promoting inflammatory regulator, and to promote tumor cell survival and reduce drug-induced apoptosis (3). These findings strongly suggested that CTRP4 is a potential therapeutic target. CTRP8 was reported to be involved in brain cancer (9). It was also demonstrated to enhance motility and matrix invasion by human glioblastoma cells (10). CTRP8-induced migration of human glioma cells was revealed to be inhibited by a small competitor peptide derived from C1QTNF6 (11). Western blotting experiments have demonstrated that C1QTNF6 is highly expressed in human hepatocellular carcinoma tissues. An immunohistochemistry assay indicated that C1QTNF6 is mainly localized in hepato-cellular carcinoma cells and endothelial cells in tumor tissues. High expression of C1QTNF6 was revealed to activate the Akt signaling pathway, increase tumor angiogenesis and reduce the necrosis of HepG2 cells (12). C1QTNF6-interference was revealed to inhibit the Erk1/2 signaling pathway in 3T3-L1 adipocytes (13). C1QTNF6 was also revealed to serve as an endogenous complement regulator that exhibited a prominent therapeutic effect in arthritis (14). It was also demonstrated that C1QTNF6 inhibited fibrogenesis by TGF-β1-stimulated human dermal fibroblasts (15).

The present study investigated the expression and localization of C1QTNF6 in GC specimens. Furthermore, the pathological functions of C1QTNF6 in GC, including cell growth, proliferation, cycle, migration and apoptosis, were studied by transfection of AGS cells with lentivirus expressing siRNA-C1QTNF6.

Materials and methods

Cell culture and regents

All cell lines were purchased from American Type Culture Collection (American Type Culture Collection, Manassas, VA, USA). SGC-7901, AGS, BGC-823 and MGC-803 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin in 5% CO2 at 37°C. Cell growth was monitored using Celigo instrument (Nexcelom Corporation, Lawrence, MA, USA). The siRNA targeting C1QTNF6 (target sequence site: TGT GTG AGA TCC CTA TGG T) was purchased from Shanghai GeneChem (Shanghai, China). SYBR-Green quantitative polymerase chain reaction (qPCR) master mix was obtained from Takara Biotechnology Co., Ltd., Dalian, China (cat. no. DRR041B). BrDU kit was obtained from Roche Diagnostics, Basel, Switzerland. FITC-Annexin V apoptosis detection kit was purchased from eBioscience; Thermo Fisher Scientific, Inc. (cat. no. 88-8007).

Patient selection

A total of 64 patients (34 males and 18 females) aged between 27 and 85 years (mean, 62.8 years) undergoing surgical resection of primary GC between March 2012 and June 2015 at the Qingdao Municipal Hospital (Qingdao, China) were selected for the present study. Gene expression profiles were generated from gastric biopsy specimens of 12 patients using GeneChip. Immunohistochemical analysis for C1QTNF6 was performed on the tumors and non-neoplastic gastric mucosal specimens (located >2 cm away from the margins of the tumor) obtained from the other 52 patients. None of the patients received chemotherapy or radiation therapy prior to surgery. The pathological diagnosis was made by 2 pathologists based on the depth of infiltration, differentiation, lymph node metastasis, microvascular invasion and nerve invasion (Table I). The Institutional Review Board at the Qingdao Municipal hospital approved the study protocol, and all patients provided written informed consent.

Table I

Pathological characteristics of gastric carcinoma (n=52).

Table I

Pathological characteristics of gastric carcinoma (n=52).

Pathological characteristicNumber%
Differentiation
 Well23.8
 Moderate1121.2
 Poor3975
Depth
 Mucosa and submucosa1121.1
 Muscularis713.5
 Serosa3465.4
Lymph node metastasis
  (+)3873.1
  (−)1426.9
Micro vessel invasion
  (+)3057.7
  (−)2242.3
Nerve invasion
  (+)3057.7
  (−)2242.3
Gene microarray analysis

Total RNA samples were extracted from tumor and peri-tumoral normal gastric tissues using TRIzol reagent (Thermo Fisher Scientific, Inc.). RNA microarray was conducted by Shanghai GeneChem. In brief, the RNA was reverse transcribed at 37°C for 60 min and then at 95°C for 5 min using the GeneChip IVT Express kit (Agilent Technologies, Inc., Santa Clara, CA, USA). The product from each reverse transcription reaction was pre-amplified and then the RNA expression was profiled using human GeneChip primeview array (Affymetrix; Thermo Fisher Scientific, Inc.), according to the manufacturer’s protocol.

Reverse transcription (RT)-qPCR analysis

Total RNA was extracted from cells and tissues using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.), according to the manufacturer’s protocol. The RNA was reverse transcribed at 42°C for 60 min using the SYBR-Green master mix in a 20-μl reaction. The primer sequences were as follows (designed and synthesized by Shanghai GeneChem): GAPDH forward, 5′-TGA CTT CAA CAG CGA CAC CCA-3′ and reverse, 5′-CAC CCT GTT GCT GTA GCC AAA-3′; and C1QTNF6 forward, 5′-GAA AGG GTC TTT GTG AAC CTT GA-3′ and reverse, 5′-CTG CGC GTA CAG GAT GAC AG-3′. The qPCR reaction cycle was performed using SYBR green master mix as follows: 10 min at 95°C and 40 cycles of 15 sec at 95°C and 60 sec at 60°C. Quantification for each sample was performed using the 2-∆∆Cq method (16).

Immunohistochemistry (IHC)

Following the tissues being fixed in 10% formalin for 24 h at room temperature and embedded in paraffin, sections from tumor and peri-tumoral normal gastric tissues were deparaffinized in xylene (15 min at room temperature, twice), rehydrated in a descending alcohol series (100%, 5 min twice; 95, 85 and 75%, 2 min each), and washed in distilled water. Prior to antigen retrieval, the tissues were treated with sodium citrate buffer (pH 6.0) at 100°C for 20 min, and endogenous hydrogen peroxidase activity was inhibited by 0.3% hydrogen peroxide. Following microwave antigen retrieval at 95-100°C, the sections were incubated with 5% FBS for 10 min at room temperature to inhibit non-specific staining. Next, the slides were incubated overnight with anti-C1QTNF6 antibody (1:1,000; ab36900; Abcam, Cambridge, UK) in a moist chamber at 4°C, followed by incubating with anti-rabbit immunoglobulin G as secondary antibody (1:2,000; ab205718; Abcam) at room temperature for 1 h. The antigen was visualized using a light microscope (magnification, ×200 or ×400).

Construction of AGS si-C1QTNF6 cell line

AGS cell lines stably expressing siRNAs were generated by transduction of 5 μg of GV115-si-C1QTNF6 or empty vector (Shanghai GeneChem) in a 100 ul transfection mix and 10 μl lipo-fectamine 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) to 1 ml of medium without serum. At 6 h following transfection, full medium was used for cell culture. Stable knockdown of the gene was determined by RT-qPCR.

Cell cycle assay

Cells were plated in 6-cm culture plates and grown to 80% confluence. Subsequently, the cells were harvested by centrifugation at 179 x g for 5 min, washed twice with PBS, fixed overnight with 75% cold ethanol at 4°C and stained with 0.05 mg/ml propidium iodide (PI) solution (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) containing 2.5 μg/ml RNase A for 40 min at 37°C. The cell cycle distribution was detected using a FACScan flow cytometer (EMD Millipore, Billerica, MA, USA) and the data was analyzed with FlowJo software 10.5.2 (Tree Star, Inc., Ashland, OR, USA).

Wound healing assay

Cells (3×104) were seeded onto 96-well plates and grown to 90% confluence. Next, the cell culture DMEM medium was changed to DMEM containing 0.5% FBS, and cells were scratched with a 96 Wounding Replicator (V&P Scientific, Inc., San Diego, CA, USA; cat. no. VP408FH) to create a mechanical wound. Images were obtained at 0, 8 and 24 h using a phase-contrast microscope (magnification, ×100). The distance of wound closure was calculated and the associated P-values calculated.

Transwell chamber invasion assay

Transwell chamber invasion assay was conducted using a Transwell kit (Corning Incorporated, Corning, NY, USA), as previously described (17). In brief, following infection with shRNA lentivirus for 3 days, the cells were harvested and suspended at a density of 1×105 cells/ml in serum-free DMEM medium. A total of 100 μl cells were placed in the upper Matrigel-coated chambers, and 600 μl DMEM medium containing 30% FBS was placed in the lower chambers. Additionally, cell suspension containing 5,000 cells was seeded into 96-well plates, and was subsequently used for measurement of OD570. After 24-h incubation at 37°C, the non-invaded cells were removed using a cotton swab, while the invaded cells were stained with 2-3 drops of Giemsa solution for 3-5 min at room temperature. The invaded cells on the lower surface of the membrane filter were counted under an inverted microscope (magnification, ×100). The data are presented as the mean number of migratory cells per field.

Colony assay

Cells (400-1,000 cells/well) were seeded into 6-well plates with three replicates. A total of 14 days after seeding, the cells were washed with PBS and fixed with 4% paraformaldehyde for 30-60 min at room temperature. The fixed cells were stained with Giemsa dyes for 10-20 min at room temperature. The colonies were visualized and counted using an inverted microscope (magnification, ×100; Shanghai Caikon Optical Instrument Co., Ltd., Shanghai, China; cat. no. XDS-100).

Proliferation assay

Cell proliferation was assessed by MTT assay or with the use of Roche BrDU kit (cat. no. 11647229001), according to the manufacturer’s protocols. For the MTT assay, 2,000 cells were seeded into 96-well plates. On the next day, 5 mg/ml MTT solution (GenView SA, Lausanne, Switzerland; cat. no. JT343) was added to each well. Four hours later, 100 μl DMSO was added to dissolve formazan particles, with votex for 2-5 min. OD490/570 of each well was read using a microplate spectrophotometer (Tecan Group Ltd., Mannedorf, Switzerland; cat. no. M2009PR). For the BrDU assay, cells were seeded into 96-well plates, and the OD450 of each well was recorded.

Apoptosis assay

Flow cytometry was used to detect the apoptotic rate. AGS cells (5×105) cells/well with or without siC1QTNF6 lentivirus infection were plated into 6-well plates and grown to 90% confluence. Following incubation, the AGS cells were harvested by centrifugation at 179 x g for 5 min and incubated with 10 μl Annexin V-APC for 10-15 min. Next, the cells were analyzed using a flow cytometer (EMD Millipore) and FlowJo software 10.5.2.

Statistical analysis

Fisher’s exact test and Student’s t-test were used to analyze the relative expression of C1QTNF6 protein in human gastric carcinoma and normal tissues. Two-way analysis of variance with a post hoc Bonferroni’s test was used to analyze the relative expression of C1QTNF6 in SGC-7901, AGS, BGC-823 and MGC-803 cell lines, and cell proliferation, migration, invasion and apoptosis. Data are presented as the mean ± standard error of the mean (n=3). All these experiments were conducted in triplicate. P<0.05 was considered to indicate a statistically significant difference. Results were analyzed using GraphPad 5.0 (GraphPad Software, Inc., La Jolla, CA, USA).

Results

C1QTNF6 gene expression was upregulated in GC tissues compared with that in peritumoral normal gastric tissues

To investigate the alterations of mRNAs, total RNA samples were isolated from tumor and the peritumoral normal gastric tissues to conduct microarray analysis. Of the ~49,395 detectable mRNAs, the expression of 238 genes had changed by >5 times. The microarray measurements are also displayed as a heat-map matrix (Fig. 1). Compared with the peritu-moral normal gastric tissues, the expression of 109 mRNAs was upregulated, while the expression of 129 mRNAs was downregulated in GC tissues. C1QTNF6 was significantly upregulated by 1.59-fold in GC tissues. The influence of the C1QTNF6 gene on GC cell proliferation, migration and invasion in vitro was investigated in the present study.

C1QTNF6 was highly expressed in GC

Next, the present study aimed to determine the expression status of C1QTNF6 in GC. Initially, the protein expression of C1QTNF6 in 52 primary GC samples and paratumoral normal gastric tissues was examined by IHC (Fig. 2A). Notably, the protein expression of C1QTNF6 was observed among GC tissues with different degrees of differentiation, including in poorly- and moderately-differentiated adenocarcinoma tissues. C1QTNF6 was primarily expressed in the cytoplasm and exhibited a diffuse granular distribution. The expression rate of C1QTNF6 [80.7% (42/52)] in GC tissues was significantly higher than that in the normal gastric tissues [23.1% (12/52); P<0.05; Fig. 2B]. There was no significant difference in C1QTNF6 expression between GC samples with different pathological grades, depth of infiltration, lymph node metastasis, lymph vascular space involvement or nerve infiltration.

Additionally, the mRNA expression profile of C1QTNF6 was also assessed in GC cell lines. The results revealed high mRNA expression of C1QTNF6 in all four GC cell lines (SGC-7901, AGS, SGC-823 and MGC-803; Fig. 2C). In the subsequent experiments, in order to assess the in vitro effect of C1QTNF6 on cell proliferation, migration, invasion and apop-tosis, the AGS cell line was used for C1QTNF6-knockdown.

Protein detection of C1QTNF6-knockdown cell lines

In order to determine whether C1QTNF6 serves a role in the growth and proliferation of GC cells, the present study first established the C1QTNF6-knockdown AGS cell line. RT-qPCR confirmed the efficiency of C1QTNF6-knockdown (Fig. 3A). The expression of C1QTNF6 in knockdown cells was 52.8% lower than that in the shCtrl cells, which indicated the successful establishment of the C1QTNF6-knockdown cell line. The growth of siC1QTNF6 and shCtrl cells from days 1 to 5 was monitored using Celigo instrument and immunofluorescence microscopy. The fluorescence intensity (Fig. 3B) and cell number (Fig. 3C) of the C1QTNF6-knockdown AGS cell line were weaker than that of the shCtrl cells.

The effect of C1QTNF6 on AGS cell proliferation was further assessed by MTT assay or using the Roche BrDU kit. For the MTT assay, 3 days after the shRNA lentivirus infection, ~2,000 cells were seeded into 96-well plates and were allowed to grow for 5 days. The AGS cell proliferation rate was significantly suppressed following C1QTNF6 interference (Fig. 3D). The results of the BrDU assay demonstrated that on the 4th day of culture, the proliferation index of AGS cells was reduced by ~27% following C1QTNF6-interference (Fig. 3E), which suggested that the proliferative ability of cells was blocked as a result of C1QTNF6-knockdown.

Subsequently, the colony formation ability of the AGS cells was investigated; the results revealed that C1QTNF6-knockdown markedly decreased the colony formation efficiency of AGS cells (P=0.00329), compared with that of cells transfected with negative control plasmid (Fig. 3F).

C1QTNF6-knockdown inhibits AGS cell invasion and migration

To detect whether C1QTNF6 affects the cell invasion ability, a Transwell chamber migration assay was conducted using AGS cells infected with siC1QTNF6 or siCtrl lentivirus. As demonstrated in Fig. 4A, infection with siC1QTNF6 notably decreased the invasive ability of AGS cells. The inhibitory rate of siC1QTNF6 on AGS cell invasion was ~76% (Fig. 4B).

Wound-healing assay revealed that the migration ability of C1QTNF6-knockdown cells at 8 and 24 h of culture was decreased by ~52.33% (P=0.0001) and ~40% (P=0.003), respectively (Fig. 4C and D); these results indicated a significant difference between the shCtrl and the siC1QTNF6 groups.

C1QTNF6-knockdown inhibits the AGS cell cycle

To investigate whether cell cycle alteration leads to decreased proliferation of siC1QTNF6 AGS cells, the cell cycle distribution was detected using a FACScan flow cytometer. Following infection with lentivirus for 5 days, there were fewer AGS cells in the S phase and more cells in the G2/M phase following C1QTNF6-knockdown (Fig. 5A and B). No significant difference was observed between the siC1QTNF6 and shCtrl groups with respect to the distribution of cells in the G1 phase, which suggested that the C1QTNF6 gene is associated with AGS cell cycle distribution.

C1QTNF6-knockdown promotes AGS cell apoptosis

In order to test the effect of C1QTNF6 on AGS cell apoptosis, flow cytometry was used to detect the apoptotic rate. Five days after transfection with lentivirus, more early apoptotic cells were observed in the siC1QTNF6 AGS group, compared with that in the shCtrl group (Fig. 6A). The apoptotic rate of siC1QTNF6 AGS cells was increased by ~62%, compared with that of the shCtrl cells (Fig. 6B), which suggested that the C1QTNF6 gene is significantly associated with AGS cell apoptosis.

Discussion

A previous study demonstrated that, under physiological conditions, CTRPs function as molecular mediators connecting inflammatory and metabolic diseases (18). In addition, CTRPs also function in tumor tissues. C1QTNF6 was revealed to be highly expressed in hepatocellular carcinoma (12), and associated with clinicopathological parameters, which suggested that C1QTNF6 is likely to be a diagnostic biomarker for hepatocellular carcinoma. Furthermore, CTRP8-induced human glioma cell migration can be inhibited by a small competitor peptide derived from C1QTNF6 (11). Taken together, these findings suggested an important role of C1QTNF6 in physiological and pathological states. The present study reported high expression levels of C1QTNF6 in GC, and C1QTNF6 was revealed to promote the proliferation and migration of GC cells in vitro and to serve a role in cell apoptosis. These results provided crucial evidence for further studies on reproduction and metastasis of GC cells in vivo. The present study provided novel evidence for the design of small molecular drugs targeting C1QTNF6 to treat GC. Furthermore, microarray assay was conducted, and numerous differentially expressed proteins were detected, which established the basis to determine the molecular mechanism of C1QTNF6 in the proliferation and migration of GC cells.

The present study demonstrated that C1QTNF6 expression in GC tissues and cell lines has an impact on cell proliferation, migration and invasion, which suggested that alternative expression of C1QTNF6 altered cancer cell life processes. However, Wang et al (19) demonstrated that downregulation of C1QTNF6 mRNA contributed toward invasiveness of the human breast cancer MCF-7 cell line. In this aforementioned study, C1QTNF6 was revealed to be directly targeted by miR-29b, which may be considered as a tumor promoter or suppressor, depending on the type of cell or tissue. Therefore, C1QTNF6 may also serve different roles in different cell types with respect to cell invasion or migration. In the present study, inhibition of C1QTNF6 induced G2-M cell cycle arrest and cell apoptosis, which suggested that C1QTNF6 sensitizes GC cells to apoptosis.

The GC AGS cells exhibited a notable decrease in wound healing ability following C1QTNF6-knockdown following 8 or 24 h culture. This may have been caused by inhibition of the expression of the potent anti-inflammatory cytokine, IL10, since C1QTNF6 can induce IL-10 expression in Raw264.7 cells (20). IL-10 was revealed to enhance wound healing of cutaneous flaps following ischemia reperfusion injury (21), and to promote regenerative healing in an adult model of scar formation (22). Additionally, numerous other factors may also be involved in regulating C1QTNF6-mediated wound healing.

The human C1QTNF family has 16 members that consist of 4 distinct domains, i.e., an N-terminal signal peptide, a short variable domain, a collagen-like domain, and a C-terminal C1q-like globular domain (23-25). A number of protein members are involved in the regulation of cell apoptosis. Administration of CTRP9 was demonstrated to attenuate cardiomyocyte apoptosis following acute myocardial infarction (26) or myocardial ischemia reperfusion (27), and to decrease the apoptotic rate of primary pulmonary artery epithelial cells due to pulmonary arterial hypertension (28). CTRP3 protected bone marrow derived mesenchymal stem cells from hypoxia-induced apoptosis through the phosphoinositide 3-kinase/protein kinase B signaling pathway (29). CTRP3 also serves a protective role in cardiac infarction through its anti-apoptotic effect in cardiomyocytes (30). CTRP4 was demonstrated to protect against apoptosis induced by chemotherapeutics and thereby promote tumor cell survival (3). The present study was the first to report the anti-apoptotic effect of C1QTNF6 in GC AGS cells; the results are consistent with those of previous studies (28-30), which suggested that C1QTNF family proteins primarily perform cytoprotective and anti-apoptotic functions. In addition, the results of the present study may be applied to a C1QTNF6-knockout animal model to extend and confirm the results derived from a cell culture model. A limitation of the present study is that the cellular protection mechanism of C1QTNF6 remains unclear. Further transcriptomic analysis in GC cells following inhibition of C1QTNF6 expression may provide further insight into the anti-apoptotic mechanism of this protein.

In conclusion, the imbalance of cell proliferation and apoptosis may contribute toward tumorigenesis, and changes in cell motility may lead to metastasis of cancer cells. C1QTNF6 affects the proliferation, migration, invasion and apoptosis of GC cells. With respect to the association between GC and C1QTNF6, the present study demonstrated the following: i) The C1QTNF6 gene is upregulated in GC tissues and cell lines; and ii) interference of C1QTNF6 expression influenced the proliferation, migration and invasion ability of GC cells in vitro. Therefore, the results of the present study provided novel evidence for GC therapy.

Acknowledgments

Not applicable.

Funding

The present study was supported by grants from Qingdao Municipal Science and Technology Bureau (grant no. 17-3-3-35-nsh).

Availability of data and materials

The datasets generated and/or analyzed during the present study are available from the corresponding author on reasonable request.

Authors’ contributions

X-JJ, DW and H-XQ designed the study. H-XQ, LC, X-YM, Z-JW, Y-XC and Y-PY collected and analyzed the data. H-XQ, LC and X-YM drafted and wrote the manuscript. X-JJ and DW revised the manuscript critically for intellectual content. All authors gave intellectual input to the study and approved the final version of the manuscript.

Ethics approval and consent to participate

The Institutional Review Board of Qingdao Municipal Hospital approved the study protocol. All procedures involving human participants were performed in accordance with the ethical standards of the Institutional and National Research Committee, and the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards. Written informed consent was obtained from all patients.

Patient consent for publication

Written informed consent was obtained from all patients.

Competing interests

The authors declare that they have no competing interests.

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January 2019
Volume 43 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Qu, H., Cui, L., Meng, X., Wang, Z., Cui, Y., Yu, Y. ... Jiang, X. (2019). C1QTNF6 is overexpressed in gastric carcinoma and contributes to the proliferation and migration of gastric carcinoma cells. International Journal of Molecular Medicine, 43, 621-629. https://doi.org/10.3892/ijmm.2018.3978
MLA
Qu, H., Cui, L., Meng, X., Wang, Z., Cui, Y., Yu, Y., Wang, D., Jiang, X."C1QTNF6 is overexpressed in gastric carcinoma and contributes to the proliferation and migration of gastric carcinoma cells". International Journal of Molecular Medicine 43.1 (2019): 621-629.
Chicago
Qu, H., Cui, L., Meng, X., Wang, Z., Cui, Y., Yu, Y., Wang, D., Jiang, X."C1QTNF6 is overexpressed in gastric carcinoma and contributes to the proliferation and migration of gastric carcinoma cells". International Journal of Molecular Medicine 43, no. 1 (2019): 621-629. https://doi.org/10.3892/ijmm.2018.3978