C1QTNF6 is overexpressed in gastric carcinoma and contributes to the proliferation and migration of gastric carcinoma cells

  • Authors:
    • Hai‑Xia Qu
    • Lin Cui
    • Xin‑Ying Meng
    • Zhi‑Jie Wang
    • Yan‑Xin Cui
    • Yun‑Peng Yu
    • Dong Wang
    • Xiang‑Jun Jiang
  • View Affiliations

  • Published online on: November 6, 2018     https://doi.org/10.3892/ijmm.2018.3978
  • Pages: 621-629
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

In the present study, proteins differentially expressed between gastric cancer tissue and para‑tumoral normal gastric tissues were screened, and the function of the highly expressed protein C1QTNF6 in gastric carcinoma was investigated. The differential expression of mRNAs extracted from the tumor and adjacent tissues was analyzed using GeneChip assay. An AGS si‑C1QTNF6 cell line was constructed using shRNA‑C1QTNF6 lentivirus. The cell invasion and migration ability of C1QTNF6‑knockdown cells were determined by Transwell chamber migration and wound healing assays, respectively. The effects of C1QTNF6 on AGS cell cycle distribution and apoptosis were detected using a FACScan flow cytometer. The results demonstrated that the expression of 109 genes was increased and the expression of 129 was decreased in tumor tissues. Among these genes, the C1QTNF6 gene was highly expressed in tumor tissues and the AGS7901 cell line. C1QTNF6‑knockdown decreased the cell growth, and the proliferative and migration ability, as well as increasing the apoptosis of gastric carcinoma cells. In addition, the number of AGS cells in the G2/M phase was significantly increased after 5 days of C1QTNF6‑shRNA lentivirus infection. The results of the present study indicated that C1QTNF6 serves an important role in the development of gastric carcinoma. C1QTNF6 is involved in promoting the proliferation and migration, and in reducing the apoptosis of gastric carcinoma cells. These results provided a potential therapeutic target for the treatment of gastric carcinoma.
View Figures
View References

Related Articles

Journal Cover

January 2019
Volume 43 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
APA
Qu, H., Cui, L., Meng, X., Wang, Z., Cui, Y., Yu, Y. ... Jiang, X. (2019). C1QTNF6 is overexpressed in gastric carcinoma and contributes to the proliferation and migration of gastric carcinoma cells. International Journal of Molecular Medicine, 43, 621-629. https://doi.org/10.3892/ijmm.2018.3978
MLA
Qu, H., Cui, L., Meng, X., Wang, Z., Cui, Y., Yu, Y., Wang, D., Jiang, X."C1QTNF6 is overexpressed in gastric carcinoma and contributes to the proliferation and migration of gastric carcinoma cells". International Journal of Molecular Medicine 43.1 (2019): 621-629.
Chicago
Qu, H., Cui, L., Meng, X., Wang, Z., Cui, Y., Yu, Y., Wang, D., Jiang, X."C1QTNF6 is overexpressed in gastric carcinoma and contributes to the proliferation and migration of gastric carcinoma cells". International Journal of Molecular Medicine 43, no. 1 (2019): 621-629. https://doi.org/10.3892/ijmm.2018.3978