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Article

Akt3 is a target of miR-29c-3p and serves an important function in the pathogenesis of congenital heart disease

  • Authors:
    • Tao Chen
    • Shu‑Jun Li
    • Bin Chen
    • Qiong Huang
    • Xiang‑Ying Kong
    • Chen Shen
    • Hai‑Tao Gu
    • Xiao‑Wei Wang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiothoracic Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of Pediatrics, Children's Hospital of Anhui Medical University, Hefei, Anhui 230000, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 980-992
    |
    Published online on: November 30, 2018
       https://doi.org/10.3892/ijmm.2018.4008
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Abstract

Our previous studies identified that the expression of microRNA‑29c (miR‑29c‑3p) was significantly increased in the serum of pregnant women carrying fetuses with congenital heart disease (CHD) compared with in that of normal pregnant women. However, the mechanism by which miR‑29c‑3p affects development of the embryonic heart remained unclear. The aim of the present study was to investigate the effect and potential molecular mechanism of miR‑29c‑3p overexpression on P19 cell proliferation, apoptosis and differentiation. miR‑29c‑3p‑overexpression and protein kinase Bγ (Akt3)‑knockdown cell lines were constructed using transfection technology. The function of miR‑29c‑3p and Akt3 in cardiomyocyte development was investigated by determining the proliferation, apoptosis and differentiation of P19 cells, which can differentiate into cardiomyocytes induced by dimethylsulfoxide. Bioinformatic analysis and luciferase assays were performed to explore the association between Akt3 and miR‑29c‑3p. The results of the present study revealed that miR‑29c‑3p overexpression and Akt3 knockdown suppressed proliferation, and promoted apoptosis and differentiation in P19 cells. Akt3 was also demonstrated to be a target of miR‑29c‑3p. Therefore, overexpression of miR‑29c‑3p may inhibit proliferation, and promote apoptosis and differentiation in P19 cells by inhibiting the expression of Akt3. miR‑29c‑3p may be a potential therapeutic target for the treatment of CHD.
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Copy and paste a formatted citation
Spandidos Publications style
Chen T, Li SJ, Chen B, Huang Q, Kong XY, Shen C, Gu HT and Wang XW: Akt3 is a target of miR-29c-3p and serves an important function in the pathogenesis of congenital heart disease. Int J Mol Med 43: 980-992, 2019.
APA
Chen, T., Li, S., Chen, B., Huang, Q., Kong, X., Shen, C. ... Wang, X. (2019). Akt3 is a target of miR-29c-3p and serves an important function in the pathogenesis of congenital heart disease. International Journal of Molecular Medicine, 43, 980-992. https://doi.org/10.3892/ijmm.2018.4008
MLA
Chen, T., Li, S., Chen, B., Huang, Q., Kong, X., Shen, C., Gu, H., Wang, X."Akt3 is a target of miR-29c-3p and serves an important function in the pathogenesis of congenital heart disease". International Journal of Molecular Medicine 43.2 (2019): 980-992.
Chicago
Chen, T., Li, S., Chen, B., Huang, Q., Kong, X., Shen, C., Gu, H., Wang, X."Akt3 is a target of miR-29c-3p and serves an important function in the pathogenesis of congenital heart disease". International Journal of Molecular Medicine 43, no. 2 (2019): 980-992. https://doi.org/10.3892/ijmm.2018.4008
Copy and paste a formatted citation
x
Spandidos Publications style
Chen T, Li SJ, Chen B, Huang Q, Kong XY, Shen C, Gu HT and Wang XW: Akt3 is a target of miR-29c-3p and serves an important function in the pathogenesis of congenital heart disease. Int J Mol Med 43: 980-992, 2019.
APA
Chen, T., Li, S., Chen, B., Huang, Q., Kong, X., Shen, C. ... Wang, X. (2019). Akt3 is a target of miR-29c-3p and serves an important function in the pathogenesis of congenital heart disease. International Journal of Molecular Medicine, 43, 980-992. https://doi.org/10.3892/ijmm.2018.4008
MLA
Chen, T., Li, S., Chen, B., Huang, Q., Kong, X., Shen, C., Gu, H., Wang, X."Akt3 is a target of miR-29c-3p and serves an important function in the pathogenesis of congenital heart disease". International Journal of Molecular Medicine 43.2 (2019): 980-992.
Chicago
Chen, T., Li, S., Chen, B., Huang, Q., Kong, X., Shen, C., Gu, H., Wang, X."Akt3 is a target of miR-29c-3p and serves an important function in the pathogenesis of congenital heart disease". International Journal of Molecular Medicine 43, no. 2 (2019): 980-992. https://doi.org/10.3892/ijmm.2018.4008
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