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Article Open Access

lncRNA SNHG1 negatively regulates miRNA‑101‑3p to enhance the expression of ROCK1 and promote cell proliferation, migration and invasion in osteosarcoma

  • Authors:
    • Rui Deng
    • Jinyuan Zhang
    • Jianting Chen
  • View Affiliations / Copyright

    Affiliations: Department of Spinal Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China
    Copyright: © Deng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1157-1166
    |
    Published online on: December 20, 2018
       https://doi.org/10.3892/ijmm.2018.4039
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Abstract

Osteosarcoma (OS) is a rare malignant bone tumor that commonly occurs in children and adolescents and causes pain and swelling of the long bones of the legs and arms. Long non‑coding RNA (lncRNA) and micro (mi)RNA‑101 are important in the initialization and progression of OS. However, the mechanism underlying the role of the lncRNA and miRNA‑101 in OS remains to be fully elucidated. In the present study, through reverse transcription‑quantitative polymerase chain reaction analysis, it was first found that the lncRNA SNHG1 was upregulated and miRNA‑101‑3p was downregulated in OS tissues and cell lines. Second, the knockdown of lncRNA SNHG1 induced cell apoptosis and maintained the cell cycle at the G0/G1 phase, which decreased the overall cell viability. Furthermore, according to a dual‑luciferase assay and western blot analysis, miRNA‑101‑3p was found to be a target of the lncRNA SNHG1 in OS, which further regulated the expression of Rho‑associated coiled‑coil‑containing protein kinase 1 (ROCK1). It was found that the phosphoinositide 3‑kinase/ATK pathway was inactivated and that epithelial‑mesenchymal transition was activated in OS cell lines with overexpression of the lncRNA SNHG1. Taken together, in OS cell lines, the lncRNA SNHG1 acted as an oncogene, and miRNA‑101‑3p was considered a tumor suppressor. The lncRNA SNHG1 promoted OS cell proliferation, migration and invasion by downregulating the expression of miRNA‑101‑3p, which enhanced the expression of ROCK1.
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Copy and paste a formatted citation
Spandidos Publications style
Deng R, Zhang J and Chen J: lncRNA SNHG1 negatively regulates miRNA‑101‑3p to enhance the expression of ROCK1 and promote cell proliferation, migration and invasion in osteosarcoma. Int J Mol Med 43: 1157-1166, 2019.
APA
Deng, R., Zhang, J., & Chen, J. (2019). lncRNA SNHG1 negatively regulates miRNA‑101‑3p to enhance the expression of ROCK1 and promote cell proliferation, migration and invasion in osteosarcoma. International Journal of Molecular Medicine, 43, 1157-1166. https://doi.org/10.3892/ijmm.2018.4039
MLA
Deng, R., Zhang, J., Chen, J."lncRNA SNHG1 negatively regulates miRNA‑101‑3p to enhance the expression of ROCK1 and promote cell proliferation, migration and invasion in osteosarcoma". International Journal of Molecular Medicine 43.3 (2019): 1157-1166.
Chicago
Deng, R., Zhang, J., Chen, J."lncRNA SNHG1 negatively regulates miRNA‑101‑3p to enhance the expression of ROCK1 and promote cell proliferation, migration and invasion in osteosarcoma". International Journal of Molecular Medicine 43, no. 3 (2019): 1157-1166. https://doi.org/10.3892/ijmm.2018.4039
Copy and paste a formatted citation
x
Spandidos Publications style
Deng R, Zhang J and Chen J: lncRNA SNHG1 negatively regulates miRNA‑101‑3p to enhance the expression of ROCK1 and promote cell proliferation, migration and invasion in osteosarcoma. Int J Mol Med 43: 1157-1166, 2019.
APA
Deng, R., Zhang, J., & Chen, J. (2019). lncRNA SNHG1 negatively regulates miRNA‑101‑3p to enhance the expression of ROCK1 and promote cell proliferation, migration and invasion in osteosarcoma. International Journal of Molecular Medicine, 43, 1157-1166. https://doi.org/10.3892/ijmm.2018.4039
MLA
Deng, R., Zhang, J., Chen, J."lncRNA SNHG1 negatively regulates miRNA‑101‑3p to enhance the expression of ROCK1 and promote cell proliferation, migration and invasion in osteosarcoma". International Journal of Molecular Medicine 43.3 (2019): 1157-1166.
Chicago
Deng, R., Zhang, J., Chen, J."lncRNA SNHG1 negatively regulates miRNA‑101‑3p to enhance the expression of ROCK1 and promote cell proliferation, migration and invasion in osteosarcoma". International Journal of Molecular Medicine 43, no. 3 (2019): 1157-1166. https://doi.org/10.3892/ijmm.2018.4039
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