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Article Open Access

Biliverdin administration regulates the microRNA-mRNA expressional network associated with neuroprotection in cerebral ischemia reperfusion injury in rats

  • Authors:
    • Zhi‑Yao Zou
    • Jia Liu
    • Cheng Chang
    • Jun‑Jie Li
    • Jing Luo
    • Yuan Jin
    • Zheng Ma
    • Ting‑Hua Wang
    • Jian‑Lin Shao
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650000, P.R. China, Experimental Animal Center, Kunming Medical University, Kunming, Yunnan 650000, P.R. China
    Copyright: © Zou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1356-1372
    |
    Published online on: January 14, 2019
       https://doi.org/10.3892/ijmm.2019.4064
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Abstract

Inflammatory response has an important role in the outcome of cerebral ischemia reperfusion injury (CIR). Biliverdin (BV) administration can relieve CIR in rats, but the mechanism remains unknown. The aim of the present study was to explore the expressional network of microRNA (miRNA)‑mRNA in CIR rats following BV administration. A rat middle cerebral artery occlusion model with BV treatment was established. After neurobehavior was evaluated by neurological severity scores (NSS), miRNA and mRNA expressional profiles were analyzed by microarray technology from the cerebral cortex subjected to ischemia and BV administration. Then, bioinformatics prediction was used to screen the correlation between miRNA and mRNA, and 20 candidate miRNAs and 33 candidate mRNAs were verified by reverse transcription‑quantitative polymerase chain reaction. Furthermore, the regulation relationship between ETS proto‑oncogene 1 (Ets1) and miRNA204‑5p was examined by luciferase assay. A total of 86 miRNAs were differentially expressed in the BV group compared with the other groups. A total of 10 miRNAs and 26 candidate genes were identified as a core ‘microRNA‑mRNA’ regulatory network that was linked with the functional improvement of BV administration in CIR rats. Lastly, the luciferase assay results confirmed that miRNA204‑5p directly targeted Ets1. The present findings suggest that BV administration may regulate multiple miRNAs and mRNAs to improve neurobehavior in CIR rats, by influencing cell proliferation, apoptosis, maintaining ATP homeostasis, and angiogenesis.
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Copy and paste a formatted citation
Spandidos Publications style
Zou ZY, Liu J, Chang C, Li JJ, Luo J, Jin Y, Ma Z, Wang TH and Shao JL: Biliverdin administration regulates the microRNA-mRNA expressional network associated with neuroprotection in cerebral ischemia reperfusion injury in rats. Int J Mol Med 43: 1356-1372, 2019.
APA
Zou, Z., Liu, J., Chang, C., Li, J., Luo, J., Jin, Y. ... Shao, J. (2019). Biliverdin administration regulates the microRNA-mRNA expressional network associated with neuroprotection in cerebral ischemia reperfusion injury in rats. International Journal of Molecular Medicine, 43, 1356-1372. https://doi.org/10.3892/ijmm.2019.4064
MLA
Zou, Z., Liu, J., Chang, C., Li, J., Luo, J., Jin, Y., Ma, Z., Wang, T., Shao, J."Biliverdin administration regulates the microRNA-mRNA expressional network associated with neuroprotection in cerebral ischemia reperfusion injury in rats". International Journal of Molecular Medicine 43.3 (2019): 1356-1372.
Chicago
Zou, Z., Liu, J., Chang, C., Li, J., Luo, J., Jin, Y., Ma, Z., Wang, T., Shao, J."Biliverdin administration regulates the microRNA-mRNA expressional network associated with neuroprotection in cerebral ischemia reperfusion injury in rats". International Journal of Molecular Medicine 43, no. 3 (2019): 1356-1372. https://doi.org/10.3892/ijmm.2019.4064
Copy and paste a formatted citation
x
Spandidos Publications style
Zou ZY, Liu J, Chang C, Li JJ, Luo J, Jin Y, Ma Z, Wang TH and Shao JL: Biliverdin administration regulates the microRNA-mRNA expressional network associated with neuroprotection in cerebral ischemia reperfusion injury in rats. Int J Mol Med 43: 1356-1372, 2019.
APA
Zou, Z., Liu, J., Chang, C., Li, J., Luo, J., Jin, Y. ... Shao, J. (2019). Biliverdin administration regulates the microRNA-mRNA expressional network associated with neuroprotection in cerebral ischemia reperfusion injury in rats. International Journal of Molecular Medicine, 43, 1356-1372. https://doi.org/10.3892/ijmm.2019.4064
MLA
Zou, Z., Liu, J., Chang, C., Li, J., Luo, J., Jin, Y., Ma, Z., Wang, T., Shao, J."Biliverdin administration regulates the microRNA-mRNA expressional network associated with neuroprotection in cerebral ischemia reperfusion injury in rats". International Journal of Molecular Medicine 43.3 (2019): 1356-1372.
Chicago
Zou, Z., Liu, J., Chang, C., Li, J., Luo, J., Jin, Y., Ma, Z., Wang, T., Shao, J."Biliverdin administration regulates the microRNA-mRNA expressional network associated with neuroprotection in cerebral ischemia reperfusion injury in rats". International Journal of Molecular Medicine 43, no. 3 (2019): 1356-1372. https://doi.org/10.3892/ijmm.2019.4064
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