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Strontium promotes osteogenic differentiation by activating autophagy via the the AMPK/mTOR signaling pathway in MC3T3‑E1 cells

  • Authors:
    • You Cheng
    • Lunhui Huang
    • Yichao Wang
    • Qianyu Huo
    • Yanhong Shao
    • Huijing Bao
    • Zhaoyang Li
    • Yunde Liu
    • Xue Li
  • View Affiliations / Copyright

    Affiliations: School of Medical Laboratory, Tianjin Medical University, Tianjin 300070, P.R. China, Department of Clinical Laboratory Medicine, Taizhou University Hospital, Taizhou, Zhejiang 318000, P.R. China, School of Materials Science and Engineering, Tianjin University, Tianjin 300350, P.R. China
    Copyright: © Cheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 652-660
    |
    Published online on: May 30, 2019
       https://doi.org/10.3892/ijmm.2019.4216
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Abstract

Strontium (Sr) is an alkaline earth metal that exerts the dual effect of improving bone formation and suppressing bone resorption, resulting in increased bone apposition rates and bone mineral density. However, the mechanisms through which Sr exerts these beneficial effects on bone have yet to be fully elucidated. The present study aimed to reveal the underlying molecular mechanisms associated with Sr‑induced osteogenic differentiation. The effects of Sr on cell proliferation and osteogenic differentiation were analyzed by MTT assay, RT‑qPCR, western blot analysis, alkaline phosphatase (ALP) and Alizarin red staining assays. The extent of autophagy was determined by monodansylcadaverine (MDC) staining and western blot analysis of two markers of cellular autophagic activity, the steatosis‑associated protein, sequestosome‑1 (SQSTM1/p62), and the two isoforms of microtubule‑associated protein 1 light chain 3 (LC3), LC‑3‑I/II. The expression levels of AMP‑activated protein kinase (AMPK) and mammalian target of rapamycin (mTOR) were also detected by western blot analysis. Sr at a concentration of 3 mM exerted the most pronounced effect on osteogenic differentiation, without any apparent cell toxicity. At the same time, cellular autophagy was active during this process. Subsequently, autophagy was blocked by 3‑methyladenine, and the enhancement of osteogenic differentiation in response to Sr was abrogated. Additionally, the phosphorylation level of AMPK was significantly increased, whereas that of mTOR was significantly decreased, in the Sr‑treated group. Taken together, the findings of the present study demonstrate that Sr stimulates AMPK‑activated autophagy to induce the osteogenic differentiation of MC3T3‑E1 cells.
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View References

1 

Chandran S, Babu SS, Vs HK, Varma HK and John A: Osteogenic efficacy of strontium hydroxyapatite micro-granules in osteopo-rotic rat model. J Biomater Appl. 31:499–509. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Querido W, Rossi AL and Farina M: The effects of strontium on bone mineral: A review on current knowledge and microanalytical approaches. Micron. 80:122–134. 2016. View Article : Google Scholar

3 

Scardueli CR, Bizelli-Silveira C, Marcantonio RAC, Marcantonio E Jr, Stavropoulos A and Spin-Neto R: Systemic administration of strontium ranelate to enhance the osseointegration of implants: Systematic review of animal studies. Int J Implant Dent. 4:212018. View Article : Google Scholar : PubMed/NCBI

4 

Reginster JY: Efficacy and safety of strontium ranelate in the treatment of knee osteoarthritis: Results of a double-blind randomised, placebo-controlled trial. Ann Rheum Dis. 73:e82014. View Article : Google Scholar

5 

Reginster JY, Beaudart C, Neuprez A and Bruyère O: Strontium ranelate in the treatment of knee osteoarthritis: New insights and emerging clinical evidence. Ther Adv Musculoskelet Dis. 5:268–276. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Chou J, Valenzuela SM, Santos J, Bishop D, Milthorpe B, Green DW, Otsuka M and Ben-Nissan B: Strontium- and magnesium-enriched biomimetic β-TCP macrospheres with potential for bone tissue morphogenesis. J Tissue Eng Regen Med. 8:771–778. 2014. View Article : Google Scholar

7 

Pasqualetti S, Banfi G and Mariotti M: The effects of strontium on skeletal development in zebrafish embryo. J Trace Elem Med Biol. 27:375–379. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Zhao S, Wang X, Li N, Chen Y, Su Y and Zhang J: Effects of strontium ranelate on bone formation in the mid-palatal suture after rapid maxillary expansion. Drug Des Devel Ther. 9:2725–2734. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Henriques Lourenço A, Neves N, Ribeiro-Machado C, Sousa SR, Lamghari M, Barrias CC, Trigo Cabral A, Barbosa MA and Ribeiro CC: Injectable hybrid system for strontium local delivery promotes bone regeneration in a rat critical-sized defect model. Sci Rep. 7:50982017. View Article : Google Scholar : PubMed/NCBI

10 

Khan PK, Mahato A, Kundu B, Nandi SK, Mukherjee P, Datta S, Sarkar S, Mukherjee J, Nath S, Balla VK and Mandal C: Influence of single and binary doping of strontium and lithium on in vivo biological properties of bioactive glass scaffolds. Sci Rep. 6:329642016. View Article : Google Scholar : PubMed/NCBI

11 

Mizushima N and Levine B: Autophagy in mammalian development and differentiation. Nat Cell Biol. 12:823–830. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Wan Y, Zhuo N, Li Y, Zhao W and Jiang D: Autophagy promotes osteogenic differentiation of human bone marrow mesenchymal stem cell derived from osteoporotic vertebrae. Biochem Biophys Res Commun. 488:46–52. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Piemontese M, Onal M, Xiong J, Han L, Thostenson JD, Almeida M and O'Brien CA: Low bone mass and changes in the osteocyte network in mice lacking autophagy in the osteoblast lineage. Sci Rep. 6:242622016. View Article : Google Scholar : PubMed/NCBI

14 

Pantovic A, Krstic A, Janjetovic K, Kocic J, Harhaji-Trajkovic L, Bugarski D and Trajkovic V: Coordinated time-dependent modulation of AMPK/Akt/mTOR signaling and autophagy controls osteogenic differentiation of human mesenchymal stem cells. Bone. 52:524–531. 2013. View Article : Google Scholar

15 

Wu SB and Wei YH: AMPK-mediated increase of glycolysis as an adaptive response to oxidative stress in human cells: Implication of the cell survival in mitochondrial diseases. Biochim Biophys Acta. 1822:233–247. 2012. View Article : Google Scholar

16 

Kim J, Kundu M, Viollet B and Guan KL: AMPK and mTOR regulate autophagy through direct phosphorylation of Ulk1. Nat Cell Biol. 13:132–141. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

18 

Klionsky DJ, Abdalla FC, Abeliovich H, Abraham RT, Acevedo-Arozena A, Adeli K, Agholme L, Agnello M, Agostinis P, Aguirre-Ghiso JA, et al: Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy. 8:445–544. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Vinod V, Padmakrishnan CJ, Vijayan B and Gopala S: 'How can I halt thee?' The puzzles involved in autophagic inhibition. Pharmacol Res. 82:1–8. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Scaglione M, Fabbri L, Casella F and Guido G: Strontium ranelate as an adjuvant for fracture healing: Clinical, radiological, and ultrasound findings in a randomized controlled study on wrist fractures. Osteoporos Int. 27:211–218. 2016. View Article : Google Scholar

21 

Chao K, Xuxia W, Qianqian W, Yuanyuan H, Shuya Z and Jun Z: Effects of strontium ranelate on the rats' palatal suture after rapid maxillary expansion. Hua Xi Kou Qiang Yi Xue Za Zhi. 34:336–340. 2016.In Chinese.

22 

Querido W, Farina M and Anselme K: Strontium ranelate improves the interaction of osteoblastic cells with titanium substrates: Increase in cell proliferation, differentiation and matrix mineralization. Biomatter. 5:e10278472015. View Article : Google Scholar : PubMed/NCBI

23 

Caverzasio J and Thouverey C: Activation of FGF receptors is a new mechanism by which strontium ranelate induces osteoblastic cell growth. Cell Physiol Biochem. 27:243–250. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Geng Z, Wang X, Zhao J, Li Z, Ma L, Zhu S, Liang Y, Cui Z, He H and Yang X: The synergistic effect of strontium-substituted hydroxyapatite and microRNA-21 on improving bone remodeling and osseointegration. Biomater Sci. 6:2694–2703. 2018. View Article : Google Scholar : PubMed/NCBI

25 

Chen YP, Tan A, Ho WP, Chuang TY, Chen WC and Chen CH: Effectiveness of strontium ranelate in the treatment of rat model of legg-calve-perthes disease. Indian J Orthop. 52:380–386. 2018. View Article : Google Scholar : PubMed/NCBI

26 

Bakhit A, Kawashima N, Hashimoto K, Noda S, Nara K, Kuramoto M, Tazawa K and Okiji T: Strontium ranelate promotes odonto-/osteogenic differentiation/mineralization of dental papillae cells in vitro and mineralized tissue formation of the dental pulp in vivo. Sci Rep. 8:92242018. View Article : Google Scholar : PubMed/NCBI

27 

Guo X, Wei S, Lu M, Shao Z, Lu J, Xia L, Lin K and Zou D: Dose-dependent effects of strontium ranelate on ovariectomy rat bone marrow mesenchymal stem cells and human umbilical vein endothelial cells. Int J Biol Sci. 12:1511–1522. 2016. View Article : Google Scholar : PubMed/NCBI

28 

Qi HH, Bao J, Zhang Q, Ma B, Gu GY, Zhang PL, Ou-Yang G, Wu ZM, Ying HJ and Ou-Yang PK: Wnt/β-catenin signaling plays an important role in the protective effects of FDP-Sr against oxidative stress induced apoptosis in MC3T3-E1 cell. Bioorg Med Chem Lett. 26:4720–4723. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Sun T, Li Z, Zhong X, Cai Z, Ning Z, Hou T, Xiong L, Feng Y, Leung F, Lu WW and Peng S: Strontium inhibits osteoclastogenesis by enhancing LRP6 and β-catenin-mediated OPG targeted by miR-181d-5p. J Cell Commun Signal. 13:85–97. 2019. View Article : Google Scholar

30 

Geng T, Sun S, Yu H, Guo H, Zheng M, Zhang S, Chen X and Jin Q: Strontium ranelate inhibits wear particle-induced aseptic loosening in mice. Braz J Med Biol Res. 51:e74142018. View Article : Google Scholar : PubMed/NCBI

31 

Reginster JY, Brandi ML, Cannata-Andia J, Cooper C, Cortet B, Feron JM, Genant H, Palacios S, Ringe JD and Rizzoli R: The position of strontium ranelate in today's management of osteoporosis. Osteoporos Int. 26:1667–1671. 2015. View Article : Google Scholar : PubMed/NCBI

32 

Cianferotti L, D'Asta F and Brandi ML: A review on strontium ranelate long-term antifracture efficacy in the treatment of postmenopausal osteoporosis. Ther Adv Musculoskelet Dis. 5:127–139. 2013. View Article : Google Scholar : PubMed/NCBI

33 

Atteritano M, Catalano A, Santoro D, Lasco A and Benvenga S: Effects of strontium ranelate on markers of cardiovascular risk in postmenopausal osteoporotic women. Endocrine. 53:305–312. 2016. View Article : Google Scholar

34 

Kim KH and Lee MS: Autophagy-a key player in cellular and body metabolism. Nat Rev Endocrinol. 10:322–337. 2014. View Article : Google Scholar : PubMed/NCBI

35 

Parzych KR and Klionsky DJ: An overview of autophagy: Morphology, mechanism, and regulation. Antioxid Redox Signal. 20:460–473. 2014. View Article : Google Scholar :

36 

Liu F, Fang F, Yuan H, Yang D, Chen Y, Williams L, Goldstein SA, Krebsbach PH and Guan JL: Suppression of autophagy by FIP200 deletion leads to osteopenia in mice through the inhibition of osteoblast terminal differentiation. J Bone Miner Res. 28:2414–2430. 2013. View Article : Google Scholar : PubMed/NCBI

37 

Gómez-Puerto MC, Verhagen LP, Braat AK, Lam EW, Coffer PJ and Lorenowicz MJ: Activation of autophagy by FOXO3 regulates redox homeostasis during osteogenic differentiation. Autophagy. 12:1804–1816. 2016. View Article : Google Scholar : PubMed/NCBI

38 

Kang X, Yang W, Feng D, Jin X, Ma Z, Qian Z, Xie T, Li H, Liu J, Wang R, et al: Cartilage- specific autophagy deficiency promotes ER stress and impairs chondrogenesis in PERK-ATF4-CHOP-dependent manner. J Bone Miner Res. 32:2128–2141. 2017. View Article : Google Scholar : PubMed/NCBI

39 

Sul OJ, Park HJ, Son HJ and Choi HS: Lipopolysaccharide (LPS)-induced autophagy is responsible for enhanced osteoclastogenesis. Mol Cells. 40:880–887. 2017.PubMed/NCBI

40 

Hu XK, Yin XH, Zhang HQ, Guo CF and Tang MX: Liraglutide attenuates the osteoblastic differentiation of MC3T3-E1 cells by modulating AMPK/mTOR signaling. Mol Med Rep. 14:3662–3668. 2016. View Article : Google Scholar : PubMed/NCBI

41 

Chen H, Liu X, Chen H, Cao J, Zhang L, Hu X and Wang J: Role of SIRT1 and AMPK in mesenchymal stem cells differentiation. Ageing Res Rev. 13:55–64. 2014. View Article : Google Scholar

42 

Wang YG, Qu XH, Yang Y, Han XG, Wang L, Qiao H, Fan QM, Tang TT and Dai KR: AMPK promotes osteogenesis and inhibits adipogenesis through AMPK- Gfi1- OPN axis. Cell Signal. 28:1270–1282. 2016. View Article : Google Scholar : PubMed/NCBI

43 

Kim EK, Lim S, Park JM, Seo JK, Kim JH, Kim KT, Ryu SH and Suh PG: Human mesenchymal stem cell differentiation to the osteogenic or adipogenic lineage is regulated by AMP-activated protein kinase. J Cell Physiol. 227:1680–1687. 2012. View Article : Google Scholar

44 

Jang WG, Kim EJ, Bae IH, Lee KN, Kim YD, Kim DK, Kim SH, Lee CH, Franceschi RT, Choi HS and Koh JT: Metformin induces osteoblast differentiation via orphan nuclear receptor SHP-mediated transactivation of Runx2. Bone. 48:885–893. 2011. View Article : Google Scholar

45 

Jang WG, Kim EJ, Lee KN, Son HJ and Koh JT: AMP-activated protein kinase (AMPK) positively regulates osteoblast differentiation via induction of Dlx5-dependent Runx2 expression in MC3T3E1 cells. Biochem Biophys Res Commun. 404:1004–1009. 2011. View Article : Google Scholar

46 

Heras-Sandoval D, Pérez-Rojas JM, Hernández-Damián J and Pedraza-Chaverri J: The role of PI3K/AKT/mTOR pathway in the modulation of autophagy and the clearance of protein aggregates in neurodegeneration. Cell Signal. 26:2694–2701. 2014. View Article : Google Scholar : PubMed/NCBI

47 

Chen J and Long F: mTORC1 signaling promotes osteoblast differentiation from preosteoblasts. PLoS One. 10:e1306272015.

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Copy and paste a formatted citation
Spandidos Publications style
Cheng Y, Huang L, Wang Y, Huo Q, Shao Y, Bao H, Li Z, Liu Y and Li X: Strontium promotes osteogenic differentiation by activating autophagy via the the AMPK/mTOR signaling pathway in MC3T3‑E1 cells. Int J Mol Med 44: 652-660, 2019.
APA
Cheng, Y., Huang, L., Wang, Y., Huo, Q., Shao, Y., Bao, H. ... Li, X. (2019). Strontium promotes osteogenic differentiation by activating autophagy via the the AMPK/mTOR signaling pathway in MC3T3‑E1 cells. International Journal of Molecular Medicine, 44, 652-660. https://doi.org/10.3892/ijmm.2019.4216
MLA
Cheng, Y., Huang, L., Wang, Y., Huo, Q., Shao, Y., Bao, H., Li, Z., Liu, Y., Li, X."Strontium promotes osteogenic differentiation by activating autophagy via the the AMPK/mTOR signaling pathway in MC3T3‑E1 cells". International Journal of Molecular Medicine 44.2 (2019): 652-660.
Chicago
Cheng, Y., Huang, L., Wang, Y., Huo, Q., Shao, Y., Bao, H., Li, Z., Liu, Y., Li, X."Strontium promotes osteogenic differentiation by activating autophagy via the the AMPK/mTOR signaling pathway in MC3T3‑E1 cells". International Journal of Molecular Medicine 44, no. 2 (2019): 652-660. https://doi.org/10.3892/ijmm.2019.4216
Copy and paste a formatted citation
x
Spandidos Publications style
Cheng Y, Huang L, Wang Y, Huo Q, Shao Y, Bao H, Li Z, Liu Y and Li X: Strontium promotes osteogenic differentiation by activating autophagy via the the AMPK/mTOR signaling pathway in MC3T3‑E1 cells. Int J Mol Med 44: 652-660, 2019.
APA
Cheng, Y., Huang, L., Wang, Y., Huo, Q., Shao, Y., Bao, H. ... Li, X. (2019). Strontium promotes osteogenic differentiation by activating autophagy via the the AMPK/mTOR signaling pathway in MC3T3‑E1 cells. International Journal of Molecular Medicine, 44, 652-660. https://doi.org/10.3892/ijmm.2019.4216
MLA
Cheng, Y., Huang, L., Wang, Y., Huo, Q., Shao, Y., Bao, H., Li, Z., Liu, Y., Li, X."Strontium promotes osteogenic differentiation by activating autophagy via the the AMPK/mTOR signaling pathway in MC3T3‑E1 cells". International Journal of Molecular Medicine 44.2 (2019): 652-660.
Chicago
Cheng, Y., Huang, L., Wang, Y., Huo, Q., Shao, Y., Bao, H., Li, Z., Liu, Y., Li, X."Strontium promotes osteogenic differentiation by activating autophagy via the the AMPK/mTOR signaling pathway in MC3T3‑E1 cells". International Journal of Molecular Medicine 44, no. 2 (2019): 652-660. https://doi.org/10.3892/ijmm.2019.4216
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