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miR‑134‑5p/Foxp2/Syn1 is involved in cognitive impairment in an early vascular dementia rat model

  • Authors:
    • Xin Liu
    • Ruilin Zhang
    • Zimei Wu
    • Wenwen Si
    • Zhenxing Ren
    • Saixia Zhang
    • Jianhong Zhou
    • Dongfeng Chen
  • View Affiliations / Copyright

    Affiliations: Department of Anatomy, The Research Center of Basic Integrative Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1729-1740
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    Published online on: September 5, 2019
       https://doi.org/10.3892/ijmm.2019.4331
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Abstract

Forkhead box P2 (Foxp2) is a transcription factor involved in vocal learning. However, the number of previous studies that have investigated the role of Foxp2 in early vascular dementia (VD) is limited. The aim of the present study was to determine whether microRNA (miR)‑134‑5p/Foxp2 contributes to cognitive impairment in a chronic ischemia‑induced early VD model. miR‑134‑5p was found to be significantly increased in the cortex in a rat VD model. Intracerebroventricular injection of miR‑134‑5p antagomir into VD rats prevented the loss of synaptic proteins and the development of cognitive impairment phenotypes. Histopathological analysis revealed that miR‑134‑5p aggravated cognitive impairment in VD rats through damage to cortical neurons and loss of synaptic proteins. Bioinformatics analysis predicted that miR‑134‑5p targets Foxp2 mRNA. Dual luciferase analysis and western blotting supported the prediction that miR‑134‑5p targets Foxp2. Furthermore, the silencing of Foxp2 significantly inhibited the effect of miR‑134‑5p on synaptic protein loss. Chromatin immunoprecipitation‑quantitative polymerase chain reaction analysis indicated that Foxp2 binds to the synapsin I (Syn1) promoter at ‑400/‑600 bp upstream of the transcription start site. In conclusion, the miR‑134‑5p/Foxp2/Syn1 axis was found to contribute to cognitive impairment in a chronic ischemia‑induced early VD model, which may enable the development of new therapeutic strategies for the prevention and treatment of VD.
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Copy and paste a formatted citation
Spandidos Publications style
Liu X, Zhang R, Wu Z, Si W, Ren Z, Zhang S, Zhou J and Chen D: miR‑134‑5p/Foxp2/Syn1 is involved in cognitive impairment in an early vascular dementia rat model. Int J Mol Med 44: 1729-1740, 2019.
APA
Liu, X., Zhang, R., Wu, Z., Si, W., Ren, Z., Zhang, S. ... Chen, D. (2019). miR‑134‑5p/Foxp2/Syn1 is involved in cognitive impairment in an early vascular dementia rat model. International Journal of Molecular Medicine, 44, 1729-1740. https://doi.org/10.3892/ijmm.2019.4331
MLA
Liu, X., Zhang, R., Wu, Z., Si, W., Ren, Z., Zhang, S., Zhou, J., Chen, D."miR‑134‑5p/Foxp2/Syn1 is involved in cognitive impairment in an early vascular dementia rat model". International Journal of Molecular Medicine 44.5 (2019): 1729-1740.
Chicago
Liu, X., Zhang, R., Wu, Z., Si, W., Ren, Z., Zhang, S., Zhou, J., Chen, D."miR‑134‑5p/Foxp2/Syn1 is involved in cognitive impairment in an early vascular dementia rat model". International Journal of Molecular Medicine 44, no. 5 (2019): 1729-1740. https://doi.org/10.3892/ijmm.2019.4331
Copy and paste a formatted citation
x
Spandidos Publications style
Liu X, Zhang R, Wu Z, Si W, Ren Z, Zhang S, Zhou J and Chen D: miR‑134‑5p/Foxp2/Syn1 is involved in cognitive impairment in an early vascular dementia rat model. Int J Mol Med 44: 1729-1740, 2019.
APA
Liu, X., Zhang, R., Wu, Z., Si, W., Ren, Z., Zhang, S. ... Chen, D. (2019). miR‑134‑5p/Foxp2/Syn1 is involved in cognitive impairment in an early vascular dementia rat model. International Journal of Molecular Medicine, 44, 1729-1740. https://doi.org/10.3892/ijmm.2019.4331
MLA
Liu, X., Zhang, R., Wu, Z., Si, W., Ren, Z., Zhang, S., Zhou, J., Chen, D."miR‑134‑5p/Foxp2/Syn1 is involved in cognitive impairment in an early vascular dementia rat model". International Journal of Molecular Medicine 44.5 (2019): 1729-1740.
Chicago
Liu, X., Zhang, R., Wu, Z., Si, W., Ren, Z., Zhang, S., Zhou, J., Chen, D."miR‑134‑5p/Foxp2/Syn1 is involved in cognitive impairment in an early vascular dementia rat model". International Journal of Molecular Medicine 44, no. 5 (2019): 1729-1740. https://doi.org/10.3892/ijmm.2019.4331
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