Open Access

lncRNA/mRNA profiling of endometriosis rat uterine tissues during the implantation window

  • Authors:
    • Han Cai
    • Xinxin Zhu
    • Zhanfei Li
    • Yapei Zhu
    • Jinghe Lang
  • View Affiliations

  • Published online on: October 14, 2019     https://doi.org/10.3892/ijmm.2019.4370
  • Pages: 2145-2160
  • Copyright: © Cai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Endometriosis is associated with changes in long non‑coding RNA (lncRNA) and mRNA expression, but the exact changes during the implantation window are unknown. Therefore, this study aimed to explore the lncRNA and mRNA expression profiles in the uterus of rats with endometriosis during the implantation window. A total of 35 non‑pregnant female rats were randomized to the endometriosis (n=13), adipose tissue control (n=8) and blank control (n=14) groups. On the 5th day of pregnancy, the rats were sacrificed to obtain uterine tissues. lncRNA and mRNA were analyzed using gene chips. A total of five differentially expressed lncRNA and four mRNA were validated by reverse transcription‑quantitative (RT‑q)PCR. Immunohistochemistry and western blotting were used to determine the expression of the ADAM metallopeptidase with thrombospondin type 1 motif 7 (Adamts7), tumor protein p53 (Tp53), distal‑less homeobox 3 (Dlx3) and pyrimidinergic receptor P2Y6 (P2ry6) proteins. There were 115 upregulated lncRNAs, 51 downregulated lncRNAs, 97 upregulated mRNAs and 85 downregulated mRNAs in the endometriosis group. RT‑qPCR confirmed the trends for five lncRNAs and four mRNAs (Adamts7, Tp53, Dlx3 and P2ry6). The relative protein expression levels of Adamts7, P2ry6, Dlx3 and TP53 were significantly different in the endometriosis group (P<0.05 vs. controls). Bioinformatics predicted the co‑expression relationship of the selected five lncRNA and four mRNA. Gene ontology and the Kyoto Encyclopedia of Genes and Genomes predicted that Adamts7, P2ry6, Dlx3 and TP53 were involved in endometriosis‑related inflammation and reproductive pathways. In conclusion, the changes in the expression of lncRNAs, mRNAs and proteins (Adamts7, P2ry6, Dlx3 and TP53) may possibly affect endometrial receptivity in rats with endometriosis during the implantation window, probably resulting in implantation failure of the embryo.
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December-2019
Volume 44 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Cai H, Zhu X, Li Z, Zhu Y and Lang J: lncRNA/mRNA profiling of endometriosis rat uterine tissues during the implantation window. Int J Mol Med 44: 2145-2160, 2019
APA
Cai, H., Zhu, X., Li, Z., Zhu, Y., & Lang, J. (2019). lncRNA/mRNA profiling of endometriosis rat uterine tissues during the implantation window. International Journal of Molecular Medicine, 44, 2145-2160. https://doi.org/10.3892/ijmm.2019.4370
MLA
Cai, H., Zhu, X., Li, Z., Zhu, Y., Lang, J."lncRNA/mRNA profiling of endometriosis rat uterine tissues during the implantation window". International Journal of Molecular Medicine 44.6 (2019): 2145-2160.
Chicago
Cai, H., Zhu, X., Li, Z., Zhu, Y., Lang, J."lncRNA/mRNA profiling of endometriosis rat uterine tissues during the implantation window". International Journal of Molecular Medicine 44, no. 6 (2019): 2145-2160. https://doi.org/10.3892/ijmm.2019.4370