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TPX2 silencing exerts anti‑tumor effects on hepatocellular carcinoma by regulating the PI3K/AKT signaling pathway

  • Authors:
    • Dan‑Hong Huang
    • Jie Jian
    • Shuang Li
    • Yan Zhang
    • Li‑Zhen Liu
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, Jiading District Central Hospital of Shanghai University of Medicine and Health Sciences, Shanghai 201800, P.R. China, Department of Gastroenterology, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Department of Gastroenterology, Third Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330008, P.R. China, Department of Oncology, Jiading District Central Hospital of Shanghai University of Medicine and Health Sciences, Shanghai 201800, P.R. China
    Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2113-2122
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    Published online on: October 14, 2019
       https://doi.org/10.3892/ijmm.2019.4371
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Abstract

Hepatocellular carcinoma (HCC) is one of the primary causes of cancer‑associated deaths worldwide. Current treatment methods include surgical resection, chemotherapy and radiotherapy; however the curative rate remains low, thus novel treatments are required. The aim of the present study was to investigate the role of targeting protein for Xenopus kinesin‑like protein 2 (TPX2) in the growth of HCC and its underlying molecular mechanism. Immunohistochemistry staining, reverse transcription‑quantitative (RT‑q)PCR and western blotting were used to detect the expression of TPX2 mRNA and protein in liver cancer tissue samples, adjacent normal liver tissue samples, and the HCC cell lines Huh7, Hep3B, PLC/PRF/5 and MHCC97‑H. The recombinant plasmid pMagic4.1‑shRNA‑TPX2 was constructed and transfected into Huh7 and Hep3B HCC cells to silence TPX2 expression. The proliferation, apoptosis, migration and invasion of Huh7 cells and Hep3B cells were evaluated before and after TPX2 silencing. The mRNA and protein expression levels of multiple signaling pathway‑associated genes were detected by RT‑qPCR and western blotting. The expression levels of TPX2 mRNA and protein were significantly higher in HCC tissue samples compared with adjacent normal liver tissue sample. TPX2 mRNA and protein expression levels were detected in the different HCC cell lines. The recombinant plasmid pMagic4.1‑shRNA‑TPX2 was successfully transfected into Huh7 and Hep3B cells, resulting in TPX2 silencing. TPX2 knockdown significantly reduced cell proliferation, cell migration and cell invasion of Huh7 and Hep3B cells, whilst also increasing the rate of apoptosis in these cells. Following TPX2 silencing, the expression levels of PI3K, phospho‑AKT, Bcl‑2, c‑Myc and Cyclin D1 were significantly decreased, whereas the expression levels of P21 and P27 were significantly increased. In conclusion, TPX2 may suppress the growth of HCC by regulating the PI3K/AKT signaling pathway and thus, TPX2 may be a potential target for the treatment of liver cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Huang DH, Jian J, Li S, Zhang Y and Liu LZ: TPX2 silencing exerts anti‑tumor effects on hepatocellular carcinoma by regulating the PI3K/AKT signaling pathway. Int J Mol Med 44: 2113-2122, 2019.
APA
Huang, D., Jian, J., Li, S., Zhang, Y., & Liu, L. (2019). TPX2 silencing exerts anti‑tumor effects on hepatocellular carcinoma by regulating the PI3K/AKT signaling pathway. International Journal of Molecular Medicine, 44, 2113-2122. https://doi.org/10.3892/ijmm.2019.4371
MLA
Huang, D., Jian, J., Li, S., Zhang, Y., Liu, L."TPX2 silencing exerts anti‑tumor effects on hepatocellular carcinoma by regulating the PI3K/AKT signaling pathway". International Journal of Molecular Medicine 44.6 (2019): 2113-2122.
Chicago
Huang, D., Jian, J., Li, S., Zhang, Y., Liu, L."TPX2 silencing exerts anti‑tumor effects on hepatocellular carcinoma by regulating the PI3K/AKT signaling pathway". International Journal of Molecular Medicine 44, no. 6 (2019): 2113-2122. https://doi.org/10.3892/ijmm.2019.4371
Copy and paste a formatted citation
x
Spandidos Publications style
Huang DH, Jian J, Li S, Zhang Y and Liu LZ: TPX2 silencing exerts anti‑tumor effects on hepatocellular carcinoma by regulating the PI3K/AKT signaling pathway. Int J Mol Med 44: 2113-2122, 2019.
APA
Huang, D., Jian, J., Li, S., Zhang, Y., & Liu, L. (2019). TPX2 silencing exerts anti‑tumor effects on hepatocellular carcinoma by regulating the PI3K/AKT signaling pathway. International Journal of Molecular Medicine, 44, 2113-2122. https://doi.org/10.3892/ijmm.2019.4371
MLA
Huang, D., Jian, J., Li, S., Zhang, Y., Liu, L."TPX2 silencing exerts anti‑tumor effects on hepatocellular carcinoma by regulating the PI3K/AKT signaling pathway". International Journal of Molecular Medicine 44.6 (2019): 2113-2122.
Chicago
Huang, D., Jian, J., Li, S., Zhang, Y., Liu, L."TPX2 silencing exerts anti‑tumor effects on hepatocellular carcinoma by regulating the PI3K/AKT signaling pathway". International Journal of Molecular Medicine 44, no. 6 (2019): 2113-2122. https://doi.org/10.3892/ijmm.2019.4371
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