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Feasibility of tumor‑derived exosome enrichment in the onco‑hematology leukemic model of chronic myeloid leukemia

  • Authors:
    • Simona Bernardi
    • Chiara Foroni
    • Camilla Zanaglio
    • Federica Re
    • Nicola Polverelli
    • Alessandro Turra
    • Enrico Morello
    • Mirko Farina
    • Federica Cattina
    • Lisa Gandolfi
    • Tatiana Zollner
    • Eugenia Accorsi Buttini
    • Michele Malagola
    • Domenico Russo
  • View Affiliations / Copyright

    Affiliations: Chair of Hematology, Unit of Blood Diseases and Stem Cell Transplantation, Department of Clinical and Experimental Sciences, University of Brescia, Italy
    Copyright: © Bernardi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2133-2144
    |
    Published online on: October 14, 2019
       https://doi.org/10.3892/ijmm.2019.4372
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Abstract

Due to the discovery of their role in intra‑cellular communications, exosomes, which carry information specific to the cell of origin, have garnered considerable attention in cancer research. Moreover, there is evidence to suggest the possibility of isolating different exosome sub‑populations based on target antigens at the cell surface. Philadelphia chromosome‑positive (Ph+) chronic myeloid leukemia (CML) is a clonal myeloproliferative neoplasia characterized by the breakpoint cluster region‑proto‑oncogene 1 tyrosine‑protein kinase (BCR‑ABL1) fusion‑gene, derived from the t (9;22) translocation. Tyrosine kinase inhibitors (TKIs) target BCR‑ABL1 protein and induce major or deep molecular responses in the majority of patients. Despite the fact that several studies have demonstrated the persistence of leukemic cells in the bone marrow niche, even following treatment, TKIs prolong patient survival time and facilitate treatment‑free remission. These characteristics render CML a plausible model for investigating the feasibility of tumor‑derived exosome fraction enrichment. In the present study, patients in the chronic phase (CP) of CML were treated with TKIs, and the quantification of the BCR‑ABL1 exosomal transcript was performed using digital PCR (dPCR). The possibility of tumor‑derived exosomes enrichment was confirmed, and for the first time, to the best of our knowledge, the detection of the BCR‑ABL1 transcript highlighted the presence of active leukemic cells in patients with CP‑CML. According to these findings, tumor‑derived exosomes may be considered a novel tool for the identification of active leukemic cells, and for the assessment of innovative monitoring focused on the biological functions of exosomes in CML.
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Spandidos Publications style
Bernardi S, Foroni C, Zanaglio C, Re F, Polverelli N, Turra A, Morello E, Farina M, Cattina F, Gandolfi L, Gandolfi L, et al: Feasibility of tumor‑derived exosome enrichment in the onco‑hematology leukemic model of chronic myeloid leukemia. Int J Mol Med 44: 2133-2144, 2019.
APA
Bernardi, S., Foroni, C., Zanaglio, C., Re, F., Polverelli, N., Turra, A. ... Russo, D. (2019). Feasibility of tumor‑derived exosome enrichment in the onco‑hematology leukemic model of chronic myeloid leukemia. International Journal of Molecular Medicine, 44, 2133-2144. https://doi.org/10.3892/ijmm.2019.4372
MLA
Bernardi, S., Foroni, C., Zanaglio, C., Re, F., Polverelli, N., Turra, A., Morello, E., Farina, M., Cattina, F., Gandolfi, L., Zollner, T., Buttini, E. A., Malagola, M., Russo, D."Feasibility of tumor‑derived exosome enrichment in the onco‑hematology leukemic model of chronic myeloid leukemia". International Journal of Molecular Medicine 44.6 (2019): 2133-2144.
Chicago
Bernardi, S., Foroni, C., Zanaglio, C., Re, F., Polverelli, N., Turra, A., Morello, E., Farina, M., Cattina, F., Gandolfi, L., Zollner, T., Buttini, E. A., Malagola, M., Russo, D."Feasibility of tumor‑derived exosome enrichment in the onco‑hematology leukemic model of chronic myeloid leukemia". International Journal of Molecular Medicine 44, no. 6 (2019): 2133-2144. https://doi.org/10.3892/ijmm.2019.4372
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Spandidos Publications style
Bernardi S, Foroni C, Zanaglio C, Re F, Polverelli N, Turra A, Morello E, Farina M, Cattina F, Gandolfi L, Gandolfi L, et al: Feasibility of tumor‑derived exosome enrichment in the onco‑hematology leukemic model of chronic myeloid leukemia. Int J Mol Med 44: 2133-2144, 2019.
APA
Bernardi, S., Foroni, C., Zanaglio, C., Re, F., Polverelli, N., Turra, A. ... Russo, D. (2019). Feasibility of tumor‑derived exosome enrichment in the onco‑hematology leukemic model of chronic myeloid leukemia. International Journal of Molecular Medicine, 44, 2133-2144. https://doi.org/10.3892/ijmm.2019.4372
MLA
Bernardi, S., Foroni, C., Zanaglio, C., Re, F., Polverelli, N., Turra, A., Morello, E., Farina, M., Cattina, F., Gandolfi, L., Zollner, T., Buttini, E. A., Malagola, M., Russo, D."Feasibility of tumor‑derived exosome enrichment in the onco‑hematology leukemic model of chronic myeloid leukemia". International Journal of Molecular Medicine 44.6 (2019): 2133-2144.
Chicago
Bernardi, S., Foroni, C., Zanaglio, C., Re, F., Polverelli, N., Turra, A., Morello, E., Farina, M., Cattina, F., Gandolfi, L., Zollner, T., Buttini, E. A., Malagola, M., Russo, D."Feasibility of tumor‑derived exosome enrichment in the onco‑hematology leukemic model of chronic myeloid leukemia". International Journal of Molecular Medicine 44, no. 6 (2019): 2133-2144. https://doi.org/10.3892/ijmm.2019.4372
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