Open Access

Feasibility of tumor‑derived exosome enrichment in the onco‑hematology leukemic model of chronic myeloid leukemia

  • Authors:
    • Simona Bernardi
    • Chiara Foroni
    • Camilla Zanaglio
    • Federica Re
    • Nicola Polverelli
    • Alessandro Turra
    • Enrico Morello
    • Mirko Farina
    • Federica Cattina
    • Lisa Gandolfi
    • Tatiana Zollner
    • Eugenia Accorsi Buttini
    • Michele Malagola
    • Domenico Russo
  • View Affiliations

  • Published online on: October 14, 2019     https://doi.org/10.3892/ijmm.2019.4372
  • Pages: 2133-2144
  • Copyright: © Bernardi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Due to the discovery of their role in intra‑cellular communications, exosomes, which carry information specific to the cell of origin, have garnered considerable attention in cancer research. Moreover, there is evidence to suggest the possibility of isolating different exosome sub‑populations based on target antigens at the cell surface. Philadelphia chromosome‑positive (Ph+) chronic myeloid leukemia (CML) is a clonal myeloproliferative neoplasia characterized by the breakpoint cluster region‑proto‑oncogene 1 tyrosine‑protein kinase (BCR‑ABL1) fusion‑gene, derived from the t (9;22) translocation. Tyrosine kinase inhibitors (TKIs) target BCR‑ABL1 protein and induce major or deep molecular responses in the majority of patients. Despite the fact that several studies have demonstrated the persistence of leukemic cells in the bone marrow niche, even following treatment, TKIs prolong patient survival time and facilitate treatment‑free remission. These characteristics render CML a plausible model for investigating the feasibility of tumor‑derived exosome fraction enrichment. In the present study, patients in the chronic phase (CP) of CML were treated with TKIs, and the quantification of the BCR‑ABL1 exosomal transcript was performed using digital PCR (dPCR). The possibility of tumor‑derived exosomes enrichment was confirmed, and for the first time, to the best of our knowledge, the detection of the BCR‑ABL1 transcript highlighted the presence of active leukemic cells in patients with CP‑CML. According to these findings, tumor‑derived exosomes may be considered a novel tool for the identification of active leukemic cells, and for the assessment of innovative monitoring focused on the biological functions of exosomes in CML.
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December-2019
Volume 44 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Bernardi S, Foroni C, Zanaglio C, Re F, Polverelli N, Turra A, Morello E, Farina M, Cattina F, Gandolfi L, Gandolfi L, et al: Feasibility of tumor‑derived exosome enrichment in the onco‑hematology leukemic model of chronic myeloid leukemia. Int J Mol Med 44: 2133-2144, 2019
APA
Bernardi, S., Foroni, C., Zanaglio, C., Re, F., Polverelli, N., Turra, A. ... Russo, D. (2019). Feasibility of tumor‑derived exosome enrichment in the onco‑hematology leukemic model of chronic myeloid leukemia. International Journal of Molecular Medicine, 44, 2133-2144. https://doi.org/10.3892/ijmm.2019.4372
MLA
Bernardi, S., Foroni, C., Zanaglio, C., Re, F., Polverelli, N., Turra, A., Morello, E., Farina, M., Cattina, F., Gandolfi, L., Zollner, T., Buttini, E. A., Malagola, M., Russo, D."Feasibility of tumor‑derived exosome enrichment in the onco‑hematology leukemic model of chronic myeloid leukemia". International Journal of Molecular Medicine 44.6 (2019): 2133-2144.
Chicago
Bernardi, S., Foroni, C., Zanaglio, C., Re, F., Polverelli, N., Turra, A., Morello, E., Farina, M., Cattina, F., Gandolfi, L., Zollner, T., Buttini, E. A., Malagola, M., Russo, D."Feasibility of tumor‑derived exosome enrichment in the onco‑hematology leukemic model of chronic myeloid leukemia". International Journal of Molecular Medicine 44, no. 6 (2019): 2133-2144. https://doi.org/10.3892/ijmm.2019.4372