Open Access

Icaritin promotes the osteogenesis of bone marrow mesenchymal stem cells via the regulation of sclerostin expression

  • Authors:
    • Qiushi Wei
    • Bin Wang
    • Hailan Hu
    • Chuhai Xie
    • Long Ling
    • Jianliang Gao
    • Yanming Cao
  • View Affiliations

  • Published online on: January 20, 2020     https://doi.org/10.3892/ijmm.2020.4470
  • Pages: 816-824
  • Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Icaritin, a metabolite of icariin, is a potent promoter of bone marrow‑derived mesenchymal stem cells (BMSCs) osteogenesis, but the underlying mechanisms remain unclear. To examine the effects of icaritin on osteogenic differentiation, BMSCs were exposed to osteogenic induction medium with or without icaritin pretreatment in the present study. It was identified that icaritin (0.01‑1 µM) exhibited no cytotoxicity on the proliferative abilities of the BMSCs. Icaritin at 1 µM increased alkaline phosphatase activity, mineral deposition and osteoblast‑specific gene expression. Treatment with 1 µM Icaritin upregulated osteocalcin, RUNX family transcription factor 2, tissue‑nonspecific alkaline phosphatase and β‑catenin, and suppressed sclerostin (SOST) gene expression in different stages of osteogenic differentiation. It was also demonstrated that SOST overexpression inhibited icaritin‑induced osteogenesis. The western blot analysis data suggested that ICI 182780, which causes estrogen receptor α (ERα) degradation, reversed the icaritin‑induced decrease in SOST expression, which was inconsistent with the results of immunofluorescence analysis. In conclusion, icaritin was demonstrated to promote the osteogenesis of hBMSCs by downregulating SOST expression, and icaritin‑induced suppression of SOST was regulated in part via the Wnt/β‑catenin/ERα axis.
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March-2020
Volume 45 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Wei Q, Wang B, Hu H, Xie C, Ling L, Gao J and Cao Y: Icaritin promotes the osteogenesis of bone marrow mesenchymal stem cells via the regulation of sclerostin expression. Int J Mol Med 45: 816-824, 2020
APA
Wei, Q., Wang, B., Hu, H., Xie, C., Ling, L., Gao, J., & Cao, Y. (2020). Icaritin promotes the osteogenesis of bone marrow mesenchymal stem cells via the regulation of sclerostin expression. International Journal of Molecular Medicine, 45, 816-824. https://doi.org/10.3892/ijmm.2020.4470
MLA
Wei, Q., Wang, B., Hu, H., Xie, C., Ling, L., Gao, J., Cao, Y."Icaritin promotes the osteogenesis of bone marrow mesenchymal stem cells via the regulation of sclerostin expression". International Journal of Molecular Medicine 45.3 (2020): 816-824.
Chicago
Wei, Q., Wang, B., Hu, H., Xie, C., Ling, L., Gao, J., Cao, Y."Icaritin promotes the osteogenesis of bone marrow mesenchymal stem cells via the regulation of sclerostin expression". International Journal of Molecular Medicine 45, no. 3 (2020): 816-824. https://doi.org/10.3892/ijmm.2020.4470