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miR‑539 suppresses the proliferation, migration, invasion and epithelial mesenchymal transition of pancreatic cancer cells through targeting SP1

  • Authors:
    • Liang Xue
    • Yan Shen
    • Zhenglong Zhai
    • Shusen Zheng
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China
    Copyright: © Xue et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1771-1782
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    Published online on: April 1, 2020
       https://doi.org/10.3892/ijmm.2020.4561
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Abstract

MicroRNA (miR)‑539 has inhibitory effects on certain types of cancer, but its role in pancreatic cancer (PCa) remains unclear. The present study investigated the effects of miR‑539 on PCa, and aimed to determine possible therapeutic targets for the treatment of PCa. The expression of miR‑539 in PCa tissues, paired normal adjacent tissues and PCa cell lines (CAPAN‑2, BxPC3, CFPAC1, SW1990 and PANC1), and human non‑cancerous pancreatic cells (hTRET‑HPNE) was determined and compared. The effects of upregulation and downregulation of miR‑539 on proliferation, apoptosis, cell cycle, invasion, migration and epithelial‑mesenchymal transition (EMT) of PCa cells were investigated. Additionally, the target gene of miR‑539 was predicted and its effects on PCa cells were further investigated. The results revealed low expression of miR‑539 in PCa tissues and cell lines. Additionally, increasing miR‑539 expression inhibited the proliferation, migration, invasion and EMT of PCa cells and induced apoptosis by blocking G1 phase of the cell cycle, while reducing miR‑539 expression had the opposite results. Furthermore, specificity protein 1 (SP1) was found to be the target gene of miR‑539. SP1 promoted the proliferation, migration, invasion and EMT transformation of PCa cells, but these effects were reversed by high expression of miR‑539. Additionally, miR‑539 suppressed the proliferation, metastasis, invasion and EMT transformation of PCa cells through targeting SP1. Therefore, miR‑539 overexpression may contribute toward development of novel therapeutic strategies for PCa in the future.
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Copy and paste a formatted citation
Spandidos Publications style
Xue L, Shen Y, Zhai Z and Zheng S: miR‑539 suppresses the proliferation, migration, invasion and epithelial mesenchymal transition of pancreatic cancer cells through targeting SP1. Int J Mol Med 45: 1771-1782, 2020.
APA
Xue, L., Shen, Y., Zhai, Z., & Zheng, S. (2020). miR‑539 suppresses the proliferation, migration, invasion and epithelial mesenchymal transition of pancreatic cancer cells through targeting SP1. International Journal of Molecular Medicine, 45, 1771-1782. https://doi.org/10.3892/ijmm.2020.4561
MLA
Xue, L., Shen, Y., Zhai, Z., Zheng, S."miR‑539 suppresses the proliferation, migration, invasion and epithelial mesenchymal transition of pancreatic cancer cells through targeting SP1". International Journal of Molecular Medicine 45.6 (2020): 1771-1782.
Chicago
Xue, L., Shen, Y., Zhai, Z., Zheng, S."miR‑539 suppresses the proliferation, migration, invasion and epithelial mesenchymal transition of pancreatic cancer cells through targeting SP1". International Journal of Molecular Medicine 45, no. 6 (2020): 1771-1782. https://doi.org/10.3892/ijmm.2020.4561
Copy and paste a formatted citation
x
Spandidos Publications style
Xue L, Shen Y, Zhai Z and Zheng S: miR‑539 suppresses the proliferation, migration, invasion and epithelial mesenchymal transition of pancreatic cancer cells through targeting SP1. Int J Mol Med 45: 1771-1782, 2020.
APA
Xue, L., Shen, Y., Zhai, Z., & Zheng, S. (2020). miR‑539 suppresses the proliferation, migration, invasion and epithelial mesenchymal transition of pancreatic cancer cells through targeting SP1. International Journal of Molecular Medicine, 45, 1771-1782. https://doi.org/10.3892/ijmm.2020.4561
MLA
Xue, L., Shen, Y., Zhai, Z., Zheng, S."miR‑539 suppresses the proliferation, migration, invasion and epithelial mesenchymal transition of pancreatic cancer cells through targeting SP1". International Journal of Molecular Medicine 45.6 (2020): 1771-1782.
Chicago
Xue, L., Shen, Y., Zhai, Z., Zheng, S."miR‑539 suppresses the proliferation, migration, invasion and epithelial mesenchymal transition of pancreatic cancer cells through targeting SP1". International Journal of Molecular Medicine 45, no. 6 (2020): 1771-1782. https://doi.org/10.3892/ijmm.2020.4561
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