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Article

Downregulation of miR‑141 deactivates hepatic stellate cells by targeting the PTEN/AKT/mTOR pathway

  • Authors:
    • Haijun Liang
    • Xinwei Wang
    • Changyun Si
    • Yuxiu Duan
    • Baoxin Chen
    • Haixia Liang
    • Daokun Yang
  • View Affiliations / Copyright

    Affiliations: Department of Infectious Disease, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China, Department of Neurology, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China
  • Pages: 406-414
    |
    Published online on: April 15, 2020
       https://doi.org/10.3892/ijmm.2020.4578
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Abstract

The activation of hepatic stellate cells (HSCs) caused by stimulating factors or fibrogenic cytokines is the critical stage of liver fibrosis. Recent studies have demonstrated the influence of microRNAs (miRNAs or miRs) on HSC activation and transformation; however, the function and underlying mechanisms of miRNAs in HSC activation have not yet been completely clarified. In the present study, transforming growth factor β1 (TGF‑β1) was used to treat human HSC lines (HSC‑T6 and LX2 cells) to simulate the activation of HSCs in vivo and whether the expression of miRNAs in HSCs was affected by TGF‑β1 treatment was examined using a miRNA microarray. It was observed that miR‑141 was one of the most upregulated miRNAs during HSC activation. Functional analyses revealed that miR‑141 knockdown suppressed the viability of HSCs and inhibited the expression levels of pro‑fibrotic markers. In addition, phosphatase and tensin homolog (PTEN), a well‑known suppressor of the AKT/mammalian target of rapamycin (mTOR) pathway, was found to be directly targeted by miR‑141 in HSCs. More importantly, the knockdown of PTEN markedly reversed the suppressive effects of miR‑141 inhibition on the viability of and the expression levels of pro‑fibrotic markers during HSC activation. Finally, it was observed that the downregulation of miR‑141 blocked the TGF‑β1‑induced activation of the AKT/mTOR pathway in HSCs. On the whole, the findings of the present study indicate that miR‑141 inhibition suppresses HSC activation via the AKT/mTOR pathway by targeting PTEN, highlighting that miR‑141 may serve as a novel therapeutic target for liver fibrosis.
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Copy and paste a formatted citation
Spandidos Publications style
Liang H, Wang X, Si C, Duan Y, Chen B, Liang H and Yang D: Downregulation of miR‑141 deactivates hepatic stellate cells by targeting the PTEN/AKT/mTOR pathway. Int J Mol Med 46: 406-414, 2020.
APA
Liang, H., Wang, X., Si, C., Duan, Y., Chen, B., Liang, H., & Yang, D. (2020). Downregulation of miR‑141 deactivates hepatic stellate cells by targeting the PTEN/AKT/mTOR pathway. International Journal of Molecular Medicine, 46, 406-414. https://doi.org/10.3892/ijmm.2020.4578
MLA
Liang, H., Wang, X., Si, C., Duan, Y., Chen, B., Liang, H., Yang, D."Downregulation of miR‑141 deactivates hepatic stellate cells by targeting the PTEN/AKT/mTOR pathway". International Journal of Molecular Medicine 46.1 (2020): 406-414.
Chicago
Liang, H., Wang, X., Si, C., Duan, Y., Chen, B., Liang, H., Yang, D."Downregulation of miR‑141 deactivates hepatic stellate cells by targeting the PTEN/AKT/mTOR pathway". International Journal of Molecular Medicine 46, no. 1 (2020): 406-414. https://doi.org/10.3892/ijmm.2020.4578
Copy and paste a formatted citation
x
Spandidos Publications style
Liang H, Wang X, Si C, Duan Y, Chen B, Liang H and Yang D: Downregulation of miR‑141 deactivates hepatic stellate cells by targeting the PTEN/AKT/mTOR pathway. Int J Mol Med 46: 406-414, 2020.
APA
Liang, H., Wang, X., Si, C., Duan, Y., Chen, B., Liang, H., & Yang, D. (2020). Downregulation of miR‑141 deactivates hepatic stellate cells by targeting the PTEN/AKT/mTOR pathway. International Journal of Molecular Medicine, 46, 406-414. https://doi.org/10.3892/ijmm.2020.4578
MLA
Liang, H., Wang, X., Si, C., Duan, Y., Chen, B., Liang, H., Yang, D."Downregulation of miR‑141 deactivates hepatic stellate cells by targeting the PTEN/AKT/mTOR pathway". International Journal of Molecular Medicine 46.1 (2020): 406-414.
Chicago
Liang, H., Wang, X., Si, C., Duan, Y., Chen, B., Liang, H., Yang, D."Downregulation of miR‑141 deactivates hepatic stellate cells by targeting the PTEN/AKT/mTOR pathway". International Journal of Molecular Medicine 46, no. 1 (2020): 406-414. https://doi.org/10.3892/ijmm.2020.4578
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