Open Access

Placental exosomes isolated from urine of patients with gestational diabetes exhibit a differential profile expression of microRNAs across gestation

  • Authors:
    • Ana Sofía Herrera-Van Oostdam
    • Juan Carlos Toro‑Ortíz
    • Jesús Adrián López
    • Daniel E. Noyola
    • David Alejandro García‑López
    • Noé Valentín Durán‑Figueroa
    • Eduardo Martínez‑Martínez
    • Diana P. Portales‑Pérez
    • Mariana Salgado‑Bustamante
    • Yamilé López‑Hernández
  • View Affiliations

  • Published online on: June 3, 2020     https://doi.org/10.3892/ijmm.2020.4626
  • Pages: 546-560
  • Copyright: © Herrera-Van Oostdam et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Placenta‑derived exosomes play an important role in cellular communication both in the mother and the fetus. Their concentration and composition are altered in several pregnancy disorders, such as gestational diabetes mellitus (GDM). The isolation and characterization of placental exosomes from serum, plasma and tissues from patients with GDM have been previously described; however, to the best of our knowledge, to date, there is no study available on placental exosomes isolated from urine of patients with GDM. In the present study, placental exosomes were purified from urine the 1st, 2nd and 3rd trimester of gestation. Placental exosomes were characterized by transmission electron microscopy in cryogenic mode and by western blot analysis, confirming the presence of exosomal vesicles. The expression profile of five microRNAs (miR‑516‑5p, miR‑517‑3p, miR‑518‑5p, miR‑222‑3p and miR‑16‑5p) was determined by RT‑qPCR. In healthy pregnant women, the expression of the miRNAs increased across gestation, apart from miR‑516‑5p, which was not expressed at the 2nd trimester. All the miRNAs examined were downregulated in patients with GDM at the 3rd trimester of gestation. The downregulated miRNAs affected several metabolic pathways closely associated with the pathophysiology of GDM. This provides further evidence of the regulatory role of miRNAs in the GDM. This also suggests that the of urinary exosomes may be an excellent source of biomarkers and therapeutic targets.
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August-2020
Volume 46 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos
Herrera-Van Oostdam AS, Toro‑Ortíz JC, López JA, Noyola DE, García‑López DA, Durán‑Figueroa NV, et al: Placental exosomes isolated from urine of patients with gestational diabetes exhibit a differential profile expression of microRNAs across gestation. Int J Mol Med 46: 546-560, 2020
APA
Herrera-Van Oostdam, A.S., Toro‑Ortíz, J.C., López, J.A., Noyola, D.E., García‑López, D.A., Durán‑Figueroa, N.V. ... López‑Hernández, Y. (2020). Placental exosomes isolated from urine of patients with gestational diabetes exhibit a differential profile expression of microRNAs across gestation. International Journal of Molecular Medicine, 46, 546-560. https://doi.org/10.3892/ijmm.2020.4626
MLA
Herrera-Van Oostdam, A. S., Toro‑Ortíz, J. C., López, J. A., Noyola, D. E., García‑López, D. A., Durán‑Figueroa, N. V., Martínez‑Martínez, E., Portales‑Pérez, D. P., Salgado‑Bustamante, M., López‑Hernández, Y."Placental exosomes isolated from urine of patients with gestational diabetes exhibit a differential profile expression of microRNAs across gestation". International Journal of Molecular Medicine 46.2 (2020): 546-560.
Chicago
Herrera-Van Oostdam, A. S., Toro‑Ortíz, J. C., López, J. A., Noyola, D. E., García‑López, D. A., Durán‑Figueroa, N. V., Martínez‑Martínez, E., Portales‑Pérez, D. P., Salgado‑Bustamante, M., López‑Hernández, Y."Placental exosomes isolated from urine of patients with gestational diabetes exhibit a differential profile expression of microRNAs across gestation". International Journal of Molecular Medicine 46, no. 2 (2020): 546-560. https://doi.org/10.3892/ijmm.2020.4626