Open Access

TRAP1 attenuates H9C2 myocardial cell injury induced by extracellular acidification via the inhibition of MPTP opening

  • Authors:
    • Lingxiao Zhang
    • Lerong Liu
    • Xia Li
    • Xing Zhang
    • Jiangpei Zhao
    • Yuanyuan Luo
    • Xiangyu Guo
    • Tongfeng Zhao
  • View Affiliations

  • Published online on: June 4, 2020     https://doi.org/10.3892/ijmm.2020.4631
  • Pages: 663-674
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Extracellular acidification leads to cardiac dysfunction in numerous diseases. Mitochondrial dysfunction plays an important role in this process. However, the mechanisms through which extracellular acidification induces mitochondrial dysfunction remain unclear. Tumor necrosis factor receptor‑associated protein 1 (TRAP1) maintains mitochondrial function and cell viability in tumor and non‑tumor cells. In the present study, extracellular acidification was found to induce H9C2 cell apoptosis, mitochondrial dysfunction and TRAP1 expression. The overexpression of TRAP1 attenuated H9C2 cell injury, while the silencing of TRAP1 exacerbated it. Moreover, mitochondrial permeability transition pore (MPTP) opening, which is associated with the mitochondrial apoptotic pathway and cell death, was also increased in acidic medium. The overexpression of TRAP1 inhibited MPTP opening, while the silencing of TRAP1 promoted it. The protective effect of TRAP1 on cardiomyocytes was abolished by the addition of a specific MPTP opening promoter. Similarly, a specific MPTP opening inhibitor reversed cell injury by silencing TRAP1. Taken together, the findings of the present study demonstrate that TRAP1 attenuates H9C2 cell injury induced by extracellular acidification by inhibiting MPTP opening.
View Figures
View References

Related Articles

Journal Cover

August-2020
Volume 46 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos
Zhang L, Liu L, Li X, Zhang X, Zhao J, Luo Y, et al: TRAP1 attenuates H9C2 myocardial cell injury induced by extracellular acidification via the inhibition of MPTP opening. Int J Mol Med 46: 663-674, 2020
APA
Zhang, L., Liu, L., Li, X., Zhang, X., Zhao, J., Luo, Y. ... Zhao, T. (2020). TRAP1 attenuates H9C2 myocardial cell injury induced by extracellular acidification via the inhibition of MPTP opening. International Journal of Molecular Medicine, 46, 663-674. https://doi.org/10.3892/ijmm.2020.4631
MLA
Zhang, L., Liu, L., Li, X., Zhang, X., Zhao, J., Luo, Y., Guo, X., Zhao, T."TRAP1 attenuates H9C2 myocardial cell injury induced by extracellular acidification via the inhibition of MPTP opening". International Journal of Molecular Medicine 46.2 (2020): 663-674.
Chicago
Zhang, L., Liu, L., Li, X., Zhang, X., Zhao, J., Luo, Y., Guo, X., Zhao, T."TRAP1 attenuates H9C2 myocardial cell injury induced by extracellular acidification via the inhibition of MPTP opening". International Journal of Molecular Medicine 46, no. 2 (2020): 663-674. https://doi.org/10.3892/ijmm.2020.4631