Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
September-2020 Volume 46 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September-2020 Volume 46 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Downregulation of microRNA‑143 promotes osteogenic differentiation of human adipose‑derived mesenchymal stem cells through the k‑Ras/MEK/ERK signaling pathway

  • Authors:
    • Yiming Zhang
    • Kaifeng Zhou
    • Liang Wu
    • Huijie Gu
    • Zhongyue Huang
    • Jun Xu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Minhang Hospital, Fudan University, Shanghai 201199, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 965-976
    |
    Published online on: June 19, 2020
       https://doi.org/10.3892/ijmm.2020.4651
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

MicroRNAs (miRNAs) are known to have regulatory roles in the osteogenic differentiation of various mesenchymal stem cells (MSCs), although their regulatory role on human adipose‑derived mesenchymal stem cells (hADSCs) remains unclear. The aim of the present study was to investigate the biological function and underlying molecular mechanism of miRNAs in regulating the osteogenic differentiation of hADSCs using microarray assay. hADSCs differentiated into osteoblasts under culture with osteogenic medium, with an increase observed in calcium deposits and alkaline phosphatase activity. The mRNA levels of bone sialoprotein, osteopontin and osteocalcin increased, whereas Runt‑related transcription factor‑2 expression decreased during osteogenic differentiation. In addition, miR‑143 was markedly downregulated during osteogenic differentiation, while miR‑143 overexpression inhibited and miR‑143 knockdown enhanced this process. miR‑143 overexpression also blocked extracellular signal‑regulated kinase 1/2 (ERK1/2) pathway activation, while miR‑143 inhibition enhanced it. The promoting effects of miR‑143 knockdown on the osteogenic differentiation of hADSCs were partly diminished by the mitogen‑activated protein kinase (MEK) inhibitors U0126 and PD98059. Bioinformatics analysis further revealed that miR‑143 targets k‑Ras and directly binds to the 3'‑untranslated region of its mRNA. Inhibition of miR‑143 enhanced the activation of the k‑Ras/MEK/ERK pathway during osteogenic differentiation, whereas miR‑143 overexpression had the opposite effect. Collectively, these results demonstrated that miR‑143 negatively regulates the osteogenic differentiation of hADSCs through the k‑Ras/MEK/ERK pathway, providing further insight into the underlying molecular mechanisms.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Locke M, Feisst V and Dunbar PR: Concise review: Human adipose-derived stem cells: Separating promise from clinical need. Stem Cells. 29:404–411. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Wang S, Qu X and Zhao RC: Mesenchymal stem cells hold promise for regenerative medicine. Front Med. 5:372–378. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Gimble JM and Guilak F: Differentiation potential of adipose derived adult stem (ADAS) cells. Curr Top Dev Biol. 58:137–160. 2003. View Article : Google Scholar

4 

Alvira-González J, Sánchez-Garcés MÀ, Cairó JR, Del Pozo MR, Sánchez CM and Gay-Escoda C: Assessment of bone regeneration using adipose-derived stem cells in critical-size alveolar ridge defects: An experimental study in a dog model. Int J Oral Maxillofac Implants. 31:196–203. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Sánchez-Garcés MÀ, Alvira-González J, Sánchez CM, Barbany Cairó JR, Del Pozo MR and Gay-Escoda C: Bone regeneration using adipose-derived stem cells with fibronectin in dehiscence-type defects associated with dental implants: An experimental study in a dog model. Int J Oral Maxillofac Implants. 32:e97–e106. 2017. View Article : Google Scholar : PubMed/NCBI

6 

Ambros V: The functions of animal microRNAs. Nature. 431:350–355. 2004. View Article : Google Scholar : PubMed/NCBI

7 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Jia J, Tian Q, Ling S, Liu Y, Yang S and Shao Z: miR-145 suppresses osteogenic differentiation by targeting Sp7. FEBS Lett. 587:3027–3031. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Wang SN, Zhao XQ, Yu B and Wang BW: miR-193a inhibits osteogenic differentiation of bone marrow-derived stroma cell via targeting HMGB1. Biochem Biophys Res Commun. 503:536–543. 2018. View Article : Google Scholar : PubMed/NCBI

10 

Li C, Yang D, Cao X, Wang F, Jiang H, Guo H, Du L, Guo Q and Yin X: LFG-500, a newly synthesized flavonoid, attenuates lipopolysaccharide-induced acute lung injury and inflammation in mice. Biochem Pharmacol. 113:57–69. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Afzal F, Pratap J, Ito K, Ito Y, Stein JL, van Wijnen AJ, Stein GS, Lian JB and Javed A: Smad function and intranuclear targeting share a Runx2 motif required for osteogenic lineage induction and BMP2 responsive transcription. J Cell Physiol. 204:63–72. 2005. View Article : Google Scholar

12 

Da Forno PD, Pringle JH, Hutchinson P, Osborn J, Huang Q, Potter L, Hancox RA, Fletcher A and Saldanha GS: WNT5A expression increases during melanoma progression and correlates with outcome. Clin Cancer Res. 14:5825–5832. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Corrêa SA and Eales KL: The Role of p38 MAPK and its substrates in neuronal plasticity and neurodegenerative disease. J Signal Transduct. 2012:6490792012. View Article : Google Scholar : PubMed/NCBI

14 

Thouverey C and Caverzasio J: The p38alpha MAPK positively regulates osteoblast function and postnatal bone acquisition. Cell Mol Life Sci. 69:3115–3125. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Ying X, Cheng S, Wang W, Lin Z, Chen Q, Zhang W, Kou D, Shen Y, Cheng X, Peng L, Zi Xu H and Zhu Lu C: Effect of lactoferrin on osteogenic differentiation of human adipose stem cells. Int Orthop. 36:647–653. 2012. View Article : Google Scholar :

16 

Li Y, Wang J, Chen G, Feng S, Wang P, Zhu X and Zhang R: Quercetin promotes the osteogenic differentiation of rat mesen-chymal stem cells via mitogen-activated protein kinase signaling. Exp Ther Med. 9:2072–2080. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Zhou C, Zhang X, Xu L, Wu T, Cui L and Xu D: Taurine promotes human mesenchymal stem cells to differentiate into osteoblast through the ERK pathway. Amino Acids. 46:1673–1680. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Ye C, Chen M, Chen E, Li W, Wang S, Ding Q, Wang C, Zhou C, Tang L, Hou W, et al: Knockdown of FOXA2 enhances the osteogenic differentiation of bone marrow-derived mesenchymal stem cells partly via activation of the ERK signalling pathway. Cell Death Dis. 9:8362018. View Article : Google Scholar : PubMed/NCBI

19 

Wang H, Li C, Li J, Zhu Y, Jia Y, Zhang Y, Zhang X, Li W, Cui L, Li W and Liu Y: Naringin enhances osteogenic differentiation through the activation of ERK signaling in human bone marrow mesenchymal stem cells. Iran J Basic Med Sci. 20:408–414. 2017.PubMed/NCBI

20 

Boquest AC, Shahdadfar A, Brinchmann JE and Collas P: Isolation of stromal stem cells from human adipose tissue. Methods Mol Biol. 325:35–46. 2006.PubMed/NCBI

21 

Li B, Qu C, Chen C, Liu Y, Akiyama K, Yang R, Chen F, Zhao Y and Shi S: Basic fibroblast growth factor inhibits osteogenic differentiation of stem cells from human exfoliated deciduous teeth through ERK signaling. Oral Dis. 18:285–292. 2012. View Article : Google Scholar

22 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

23 

Mei LL, Wang WJ, Qiu YT, Xie XF, Bai J and Shi ZZ: miR-125b-5p functions as a tumor suppressor gene partially by regulating HMGA2 in esophageal squamous cell carcinoma. PLoS One. 12:e01856362017. View Article : Google Scholar : PubMed/NCBI

24 

Liu TM and Lee EH: Transcriptional regulatory cascades in Runx2-dependent bone development. Tissue Eng Part B Rev. 19:254–263. 2013. View Article : Google Scholar :

25 

Lee MJ, Chen HT, Ho ML, Chen CH, Chuang SC, Huang SC, Fu YC, Wang GJ, Kang L and Chang JK: PPARγ silencing enhances osteogenic differentiation of human adipose-derived mesenchymal stem cells. J Cell Mol Med. 17:1188–1193. 2013.PubMed/NCBI

26 

Ying X, Chen X, Cheng S, Guo X, Chen H and Xu HZ: Phosphoserine promotes osteogenic differentiation of human adipose stromal cells through bone morphogenetic protein signalling. Cell Biol Int. 38:309–317. 2014. View Article : Google Scholar

27 

Li E, Zhang J, Yuan T and Ma B: MiR-143 suppresses osteogenic differentiation by targeting Osterix. Mol Cell Biochem. 390:69–74. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Ge C, Xiao G, Jiang D and Franceschi RT: Critical role of the extracellular signal-regulated kinase-MAPK pathway in osteoblast differentiation and skeletal development. J Cell Biol. 176:709–718. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Greenblatt MB, Shim JH and Glimcher LH: Mitogen-activated protein kinase pathways in osteoblasts. Annu Rev Cell Dev Biol. 29:63–79. 2013. View Article : Google Scholar : PubMed/NCBI

30 

Artigas N, Ureña C, Rodriguez-Carballo E, Rosa JL and Ventura F: Mitogen-activated protein kinase (MAPK)-regulated interactions between Osterix and Runx2 are critical for the transcriptional osteogenic program. J Biol Chem. 289:27105–27117. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Park S, Arai Y, Kim BJ, Bello A, Ashraf S, Park H, Park KS and Lee SH: SPRY4 suppression facilitates osteogenic differentiation in adipose-derived mesenchymal stem cells. Tissue Eng Part A. 25:1645–1657. 2019. View Article : Google Scholar

32 

Yang X, Yang Y, Zhou S, Gong X, Dai Q, Zhang P and Jiang L: Puerarin stimulates osteogenic differentiation and bone formation through the ERK1/2 and p38-MAPK signaling pathways. Curr Mol Med. 17:488–496. 2018. View Article : Google Scholar

33 

Wei K, Xie Y, Chen T, Fu B, Cui S, Wang Y, Cai G and Chen X: ERK1/2 signaling mediated naringin-induced osteogenic differentiation of immortalized human periodontal ligament stem cells. Biochem Biophys Res Commun. 489:319–325. 2017. View Article : Google Scholar : PubMed/NCBI

34 

Wei F, Liu Y, Bellail AC, Olson JJ, Sun SY, Lu G, Ding L, Yuan C, Wang G and Hao C: K-Ras mutation-mediated IGF-1-induced feedback ERK activation contributes to the rapalog resistance in pancreatic ductal adenocarcinomas. Cancer Lett. 322:58–69. 2012. View Article : Google Scholar : PubMed/NCBI

35 

Kim YJ, Bae SW, Yu SS, Bae YC and Jung JS: miR-196a regulates proliferation and osteogenic differentiation in mesenchymal stem cells derived from human adipose tissue. J Bone Miner Res. 24:816–825. 2009. View Article : Google Scholar

36 

Luzi E, Marini F, Sala SC, Tognarini I, Galli G and Brandi ML: Osteogenic differentiation of human adipose tissue-derived stem cells is modulated by the miR-26a targeting of the SMAD1 transcription factor. J Bone Miner Res. 23:287–295. 2008. View Article : Google Scholar : PubMed/NCBI

37 

Zeng Y, Qu X, Li H, Huang S, Wang S, Xu Q, Lin R, Han Q, Li J and Zhao RC: MicroRNA-100 regulates osteogenic differentiation of human adipose-derived mesenchymal stem cells by targeting BMPR2. FEBS Lett. 586:2375–2381. 2012. View Article : Google Scholar : PubMed/NCBI

38 

Zhang P, Yang W, Wang G and Li Y: miR-143 suppresses the osteogenic differentiation of dental pulp stem cells by inactivation of NF-κB signaling pathway via targeting TNF-α. Arch Oral Biol. 87:172–179. 2018. View Article : Google Scholar : PubMed/NCBI

39 

Jeon YM, Kook SH, Rho SJ, Lim SS, Choi KC, Kim HS, Kim JG and Lee JC: Fibroblast growth factor-7 facilitates osteogenic differentiation of embryonic stem cells through the activation of ERK/Runx2 signaling. Mol Cell Biochem. 382:37–45. 2013. View Article : Google Scholar : PubMed/NCBI

40 

Mei Y, Bian C, Li J, Du Z, Zhou H, Yang Z and Zhao RC: miR-21 modulates the ERK-MAPK signaling pathway by regulating SPRY2 expression during human mesenchymal stem cell differentiation. J Cell Biochem. 114:1374–1384. 2013. View Article : Google Scholar

41 

Dong YZ and Hu T: Effects of miR-143 overexpression on proliferation, apoptosis, EGFR and downstream signaling pathways in PC9/GR cell line. Eur Rev Med Pharmacol Sci. 22:1709–1716. 2018.PubMed/NCBI

42 

Castellano E and Santos E: Functional specificity of ras isoforms: So similar but so different. Genes Cancer. 2:216–231. 2011. View Article : Google Scholar : PubMed/NCBI

43 

Cheng D, Zhao L, Xu Y, Ou R, Li G, Yang H and Li W: K-Ras promotes the non-small lung cancer cells survival by cooperating with sirtuin 1 and p27 under ROS stimulation. Tumour Biol. 36:7221–7232. 2015. View Article : Google Scholar : PubMed/NCBI

44 

Georgieva M, Krasteva M, Angelova E, Ralchev K, Dimitrov V, Bozhimirov S, Georgieva E and Berger MR: Analysis of the K-ras/B-raf/Erk signal cascade, p53 and CMAP as markers for tumor progression in colorectal cancer patients. Oncol Rep. 20:3–11. 2008.PubMed/NCBI

45 

Feng L, Xue D, Chen E, Zhang W, Gao X, Yu J, Feng Y and Pan Z: HMGB1 promotes the secretion of multiple cytokines and potentiates the osteogenic differentiation of mesenchymal stem cells through the Ras/MAPK signaling pathway. Exp Ther Med. 12:3941–3947. 2016. View Article : Google Scholar

46 

Peng S, Zhou G, Luk KD, Cheung KM, Li Z, Lam WM, Zhou Z and Lu WW: Strontium promotes osteogenic differentiation of mesenchymal stem cells through the Ras/MAPK signaling pathway. Cell Physiol Biochem. 23:165–174. 2009. View Article : Google Scholar : PubMed/NCBI

47 

Qin HX, Cui HK, Pan Y, Hu RL, Zhu LH and Wang SJ: miR-143 inhibits cell proliferation through targeted regulating the expression of K-ras gene in HeLa cells. Zhonghua Zhong Liu Za Zhi. 38:893–897. 2016.In Chinese. PubMed/NCBI

48 

Takai T, Tsujino T, Yoshikawa Y, Inamoto T, Sugito N, Kuranaga Y, Heishima K, Soga T, Hayashi K, Miyata K, et al: Synthetic miR-143 exhibited an anti-cancer effect via the down-regulation of K-RAS networks of renal cell cancer cells in vitro and in vivo. Mol Ther. 27:1017–1027. 2019. View Article : Google Scholar : PubMed/NCBI

49 

Caplan AI: Review: Mesenchymal stem cells: Cell-based reconstructive therapy in orthopedics. Tissue Eng. 11:1198–1211. 2005. View Article : Google Scholar : PubMed/NCBI

50 

Arinzeh TL: Mesenchymal stem cells for bone repair: Preclinical studies and potential orthopedic applications. Foot Ankle Clin. 10:651–665. viii2005. View Article : Google Scholar : PubMed/NCBI

51 

Bhansali S, Dutta P, Kumar V, Yadav MK, Jain A, Mudaliar S, Bhansali S, Sharma RR, Jha V, Marwaha N, et al: Efficacy of autologous bone marrow-derived mesenchymal stem cell and mononuclear cell transplantation in type 2 diabetes mellitus: A randomized, placebo-controlled comparative study. Stem Cells Dev. 26:471–481. 2017. View Article : Google Scholar

52 

Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, Benhaim P, Lorenz HP and Hedrick MH: Multilineage cells from human adipose tissue: Implications for cell-based therapies. Tissue Eng. 7:211–228. 2001. View Article : Google Scholar : PubMed/NCBI

53 

Tobita M, Orbay H and Mizuno H: Adipose-derived stem cells: Current findings and future perspectives. Discov Med. 11:160–170. 2011.PubMed/NCBI

54 

Sempere JM, Martinez-Peinado P, Arribas MI, Reig JA, De La Sen ML, Zubcoff JJ, Fraga MF, Fernández AF, Santana A and Roche E: Single cell-derived clones from human adipose stem cells present different immunomodulatory properties. Clin Exp Immunol. 176:255–265. 2014. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Zhou K, Wu L, Gu H, Huang Z and Xu J: Downregulation of microRNA‑143 promotes osteogenic differentiation of human adipose‑derived mesenchymal stem cells through the k‑Ras/MEK/ERK signaling pathway. Int J Mol Med 46: 965-976, 2020.
APA
Zhang, Y., Zhou, K., Wu, L., Gu, H., Huang, Z., & Xu, J. (2020). Downregulation of microRNA‑143 promotes osteogenic differentiation of human adipose‑derived mesenchymal stem cells through the k‑Ras/MEK/ERK signaling pathway. International Journal of Molecular Medicine, 46, 965-976. https://doi.org/10.3892/ijmm.2020.4651
MLA
Zhang, Y., Zhou, K., Wu, L., Gu, H., Huang, Z., Xu, J."Downregulation of microRNA‑143 promotes osteogenic differentiation of human adipose‑derived mesenchymal stem cells through the k‑Ras/MEK/ERK signaling pathway". International Journal of Molecular Medicine 46.3 (2020): 965-976.
Chicago
Zhang, Y., Zhou, K., Wu, L., Gu, H., Huang, Z., Xu, J."Downregulation of microRNA‑143 promotes osteogenic differentiation of human adipose‑derived mesenchymal stem cells through the k‑Ras/MEK/ERK signaling pathway". International Journal of Molecular Medicine 46, no. 3 (2020): 965-976. https://doi.org/10.3892/ijmm.2020.4651
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Zhou K, Wu L, Gu H, Huang Z and Xu J: Downregulation of microRNA‑143 promotes osteogenic differentiation of human adipose‑derived mesenchymal stem cells through the k‑Ras/MEK/ERK signaling pathway. Int J Mol Med 46: 965-976, 2020.
APA
Zhang, Y., Zhou, K., Wu, L., Gu, H., Huang, Z., & Xu, J. (2020). Downregulation of microRNA‑143 promotes osteogenic differentiation of human adipose‑derived mesenchymal stem cells through the k‑Ras/MEK/ERK signaling pathway. International Journal of Molecular Medicine, 46, 965-976. https://doi.org/10.3892/ijmm.2020.4651
MLA
Zhang, Y., Zhou, K., Wu, L., Gu, H., Huang, Z., Xu, J."Downregulation of microRNA‑143 promotes osteogenic differentiation of human adipose‑derived mesenchymal stem cells through the k‑Ras/MEK/ERK signaling pathway". International Journal of Molecular Medicine 46.3 (2020): 965-976.
Chicago
Zhang, Y., Zhou, K., Wu, L., Gu, H., Huang, Z., Xu, J."Downregulation of microRNA‑143 promotes osteogenic differentiation of human adipose‑derived mesenchymal stem cells through the k‑Ras/MEK/ERK signaling pathway". International Journal of Molecular Medicine 46, no. 3 (2020): 965-976. https://doi.org/10.3892/ijmm.2020.4651
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team