Open Access

Oral cancer cell‑derived exosomes modulate natural killer cell activity by regulating the receptors on these cells

  • Authors:
    • Xueqin Zhu
    • Xing Qin
    • Xiaoning Wang
    • Yingnan Wang
    • Wei Cao
    • Jianjun Zhang
    • Wantao Chen
  • View Affiliations

  • Published online on: September 24, 2020     https://doi.org/10.3892/ijmm.2020.4736
  • Pages: 2115-2125
  • Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Oral cancer (OC) is the most common type of head and neck malignant tumor. Tumor‑derived exosomes induce a complex extracellular environment that affects tumor immunity. In the present study, exosomes were isolated from OC cell lines (WSU‑HN4 and SCC‑9) by ultrafiltration and the protein content of these oral cancer‑derived exosomes (OCEXs) was analyzed by mass spectrometry, which revealed the enrichment of transforming growth factor (TGF)‑β1. Natural killer (NK) cells were examined by flow cytometry following co‑culture with OCEXs. The expression of killer cell lectin like receptor K1 (KLRK1; also known as NKG2D, as used herein) and natural cytotoxicity triggering receptor 3 (NCR3; also known as NKp30, as used herein) in NK cells was found to be significantly upregulated following co‑culture with the OCEXs for 1 day, whereas this expression decreased at 7 days. Killer cell lectin like receptor C1 (KLRC1; also known as NKG2A; as used herein) expression exhibited an opposite trend at 1 day. In addition, NK cell cytotoxicity against the OC cells was enhanced at 1 day, but was attenuated at 7 days. TGF‑β1 inhibited the function of NK cells at 7 days, whereas it had no obvious effects at 1 and 3 days. On the whole, the findings of the present study reveal changes in NK cell function and provide new insight into NK cell dysfunction.
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December-2020
Volume 46 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Zhu X, Qin X, Wang X, Wang Y, Cao W, Zhang J and Chen W: Oral cancer cell‑derived exosomes modulate natural killer cell activity by regulating the receptors on these cells. Int J Mol Med 46: 2115-2125, 2020
APA
Zhu, X., Qin, X., Wang, X., Wang, Y., Cao, W., Zhang, J., & Chen, W. (2020). Oral cancer cell‑derived exosomes modulate natural killer cell activity by regulating the receptors on these cells. International Journal of Molecular Medicine, 46, 2115-2125. https://doi.org/10.3892/ijmm.2020.4736
MLA
Zhu, X., Qin, X., Wang, X., Wang, Y., Cao, W., Zhang, J., Chen, W."Oral cancer cell‑derived exosomes modulate natural killer cell activity by regulating the receptors on these cells". International Journal of Molecular Medicine 46.6 (2020): 2115-2125.
Chicago
Zhu, X., Qin, X., Wang, X., Wang, Y., Cao, W., Zhang, J., Chen, W."Oral cancer cell‑derived exosomes modulate natural killer cell activity by regulating the receptors on these cells". International Journal of Molecular Medicine 46, no. 6 (2020): 2115-2125. https://doi.org/10.3892/ijmm.2020.4736