Loss of FZO1 gene results in changes of cell dynamics in fission yeast

Yuan,Rongmei; Ding,Xiang; Tan,Xiumei; Hou,Yiling

doi:10.3892/ijmm.2020.4752

Loss of FZO1 gene results in changes of cell dynamics in fission yeast

Authors:
- Rongmei Yuan
- Xiang Ding
- Xiumei Tan
- Yiling Hou
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Affiliations: Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), College of Life Sciences, China West Normal University, Nanchong, Sichuan 637009, P.R. China, College of Environmental Science and Engineering, China West Normal University, Nanchong, Sichuan 637009, P.R. China
Published online on: October 12, 2020 https://doi.org/10.3892/ijmm.2020.4752
Pages: 2194-2206
Copyright: © Yuan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Mitochondrial fission and fusion dynamics are critical cellular processes, and abnormalities in these processes are associated with severe human disorders, such as Beckwith‑Wiedemann syndrome, neurodegenerative diseases, Charcot‑Marie‑Tooth disease type 6, multiple symmetric lipomatosis and microcephaly. Fuzzy onions protein 1 (Fzo1p) regulates mitochondrial outer membrane fusion. In the present study, Schizosaccharomyces pombe (S. pombe) was used to explore the effect of FZO1 gene deletion on cell dynamics in mitosis. The mitochondrial morphology results showed that the mitochondria appeared to be fragmented and tubular in wild‑type cells; however, they were observed to accumulate in fzo1Δ cells. The FZO1 gene deletion was demonstrated to result in slow proliferation, sporogenesis defects, increased microtubule (MT) number and actin contraction defects in S. pombe. The FZO1 gene deletion also affected the rate of spindle elongation and phase time at the metaphase and anaphase, as well as spindle MT organization. Live‑cell imaging was performed on mutant strains to observe three distinct kinetochore behaviors (normal, lagging and mis‑segregation), as well as abnormal spindle breakage. The FZO1 gene deletion resulted in coenzyme and intermediate metabolite abnormalities as determined via metabolomics analysis. It was concluded that the loss of FZO1 gene resulted in deficiencies in mitochondrial dynamics, which may result in deficiencies in spindle maintenance, chromosome segregation, spindle breakage, actin contraction, and coenzyme and intermediate metabolite levels.

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