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Article Open Access

Early local neutralization of CC16 in sepsis‑induced ALI following blunt chest trauma leads to delayed mortality without benefitting overall survival

  • Authors:
    • Jan Tilmann Vollrath
    • Philipp Stoermann
    • Nils Becker
    • Sebastian Wutzler
    • Frank Hildebrand
    • Ingo Marzi
    • Borna Relja
  • View Affiliations / Copyright

    Affiliations: Department of Trauma, Hand and Reconstructive Surgery, Goethe University, D‑60590 Frankfurt, Germany, Department of Trauma, Hand and Orthopedic Surgery, Helios Horst Schmidt Clinic, D‑65199 Wiesbaden, Germany, Department of Trauma Surgery, RWTH University, D‑52062 Aachen, Germany
    Copyright: © Vollrath et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2207-2215
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    Published online on: October 22, 2020
       https://doi.org/10.3892/ijmm.2020.4767
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Abstract

Blunt thoracic trauma (TxT) is a common injury pattern in polytraumatized patients. When combined with a secondary trigger, TxT often results in acute lung injury (ALI), which negatively affects outcomes. Recent findings suggest that ALI is caused by both local and systemic inflammatory reactions. Club cell protein (CC)16 is an anti‑inflammatory peptide associated with lung injury following TxT. Recently, the anti‑inflammatory properties of endogenous CC16 in a murine model of TxT with subsequent cecal‑ligation and puncture (CLP) as the secondary hit were demonstrated by our group. The present study aimed to determine whether CC16 neutralization improves survival following ‘double‑hit’‑induced ALI. For this purpose, a total of 120 C57BL/6N mice were subjected to TxT, followed by CLP after 24 h. Sham‑operated animals underwent anesthesia without the induction of TxT + CLP. CC16 neutralization was performed by providing a CC16 antibody intratracheally following TxT (early) or following CLP (late). Survival was assessed in 48 animals for 6 days after CLP. Sacrifice was performed 6 or 24 h post‑CLP to evaluate the anti‑inflammatory effect of CC16. The results revealed that CC16 neutralization enhanced pro‑inflammatory CXCL1 levels, thereby confirming the anti‑inflammatory characteristics of CC16 in this model. Early CC16 neutralization immediately following TxT significantly prolonged survival within 60 h; however, the survival rate did not change until 6 days post‑trauma. Late CC16 neutralization did not provide any survival benefits. On the whole, the present study demonstrated that neutralizing CC16 confirmed its anti‑inflammatory potential in this double‑hit ALI model. Early CC16 neutralization prolonged survival within 60 h; however, no survival benefits were observed after 6 days post‑CLP in any group.
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Copy and paste a formatted citation
Spandidos Publications style
Vollrath JT, Stoermann P, Becker N, Wutzler S, Hildebrand F, Marzi I and Relja B: Early local neutralization of CC16 in sepsis‑induced ALI following blunt chest trauma leads to delayed mortality without benefitting overall survival. Int J Mol Med 46: 2207-2215, 2020.
APA
Vollrath, J.T., Stoermann, P., Becker, N., Wutzler, S., Hildebrand, F., Marzi, I., & Relja, B. (2020). Early local neutralization of CC16 in sepsis‑induced ALI following blunt chest trauma leads to delayed mortality without benefitting overall survival. International Journal of Molecular Medicine, 46, 2207-2215. https://doi.org/10.3892/ijmm.2020.4767
MLA
Vollrath, J. T., Stoermann, P., Becker, N., Wutzler, S., Hildebrand, F., Marzi, I., Relja, B."Early local neutralization of CC16 in sepsis‑induced ALI following blunt chest trauma leads to delayed mortality without benefitting overall survival". International Journal of Molecular Medicine 46.6 (2020): 2207-2215.
Chicago
Vollrath, J. T., Stoermann, P., Becker, N., Wutzler, S., Hildebrand, F., Marzi, I., Relja, B."Early local neutralization of CC16 in sepsis‑induced ALI following blunt chest trauma leads to delayed mortality without benefitting overall survival". International Journal of Molecular Medicine 46, no. 6 (2020): 2207-2215. https://doi.org/10.3892/ijmm.2020.4767
Copy and paste a formatted citation
x
Spandidos Publications style
Vollrath JT, Stoermann P, Becker N, Wutzler S, Hildebrand F, Marzi I and Relja B: Early local neutralization of CC16 in sepsis‑induced ALI following blunt chest trauma leads to delayed mortality without benefitting overall survival. Int J Mol Med 46: 2207-2215, 2020.
APA
Vollrath, J.T., Stoermann, P., Becker, N., Wutzler, S., Hildebrand, F., Marzi, I., & Relja, B. (2020). Early local neutralization of CC16 in sepsis‑induced ALI following blunt chest trauma leads to delayed mortality without benefitting overall survival. International Journal of Molecular Medicine, 46, 2207-2215. https://doi.org/10.3892/ijmm.2020.4767
MLA
Vollrath, J. T., Stoermann, P., Becker, N., Wutzler, S., Hildebrand, F., Marzi, I., Relja, B."Early local neutralization of CC16 in sepsis‑induced ALI following blunt chest trauma leads to delayed mortality without benefitting overall survival". International Journal of Molecular Medicine 46.6 (2020): 2207-2215.
Chicago
Vollrath, J. T., Stoermann, P., Becker, N., Wutzler, S., Hildebrand, F., Marzi, I., Relja, B."Early local neutralization of CC16 in sepsis‑induced ALI following blunt chest trauma leads to delayed mortality without benefitting overall survival". International Journal of Molecular Medicine 46, no. 6 (2020): 2207-2215. https://doi.org/10.3892/ijmm.2020.4767
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