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Astragaloside IV attenuates cerebral ischemia‑reperfusion injury in rats through the inhibition of calcium‑sensing receptor‑mediated apoptosis

  • Authors:
    • Shu‑Jin Du
    • Ying Zhang
    • Yan‑Meng Zhao
    • Ya‑Jie Dong
    • Jing‑Long Tang
    • Xiao‑Hong Zhou
    • Wei‑Juan Gao
  • View Affiliations / Copyright

    Affiliations: Hebei Key Laboratory of Chinese Medicine Research on Cardio‑Cerebrovascular Disease, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, P.R. China, Department of Pathophysiology, Chengde Medical College, Chengde, Hebei 067000, P.R. China, The Reproductive Genetics Laboratory, Jinan Adicon Clinical Labor, Binzhou, Shandong 256600, P.R. China
    Copyright: © Du et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 302-314
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    Published online on: November 2, 2020
       https://doi.org/10.3892/ijmm.2020.4777
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Abstract

Cerebral ischemia‑reperfusion injury (CIRI), caused by the reperfusion of blocked vessels following ischemic stroke, can lead to secondary brain injury. Throughout CIRI, apoptosis serves an important role. Astragaloside IV is a potential neuroprotectant that alleviates CIRI by inhibiting apoptosis. The calcium‑sensing receptor (CaSR) is a G‑protein‑coupled receptor, the activation of which aggravates ischemia‑reperfusion injury. The aim of the present study was to investigate whether the protective effect of Astragaloside IV on CIRI may be associated with the regulation of CaSR. A rat middle cerebral artery occlusion/reperfusion (MCAO/R) model and an oxygen and glucose deprivation/reoxygenation (OGD/R) model of pheochromocytoma (PC12) cells were used to study the neuronal injury induced by CIRI. Neurological function scores (NFS), 2,3,5‑triphe‑nylterazolium chloride and hematoxylin and eosin staining were used to determine brain damage in rats. Cell viability was measured to evaluate the injury of OGD/R PC12 cells. Western blotting was used to examine the expression of proteins associated with apoptosis and CaSR. The CaSR antagonist NPS‑2143 and agonist GdCl3 were used to further confirm the effects of CaSR during the process of apoptosis. The results demonstrated that Astragaloside IV alleviated CIRI by decreasing the NFS of rats, reducing the infarction volume of the brain and promoting the viability of PC12 cells, as well as inhibiting the expression of cleaved caspase‑3 and CaSR, which was induced by CIRI. The results of the present study suggested that the activation of CaSR may be involved in CIRI‑induced brain damage in rats, and that Astragaloside IV may alleviate CIRI by inhibiting CaSR activation‑induced apoptosis.
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Spandidos Publications style
Du SJ, Zhang Y, Zhao YM, Dong YJ, Tang JL, Zhou XH and Gao WJ: Astragaloside IV attenuates cerebral ischemia‑reperfusion injury in rats through the inhibition of calcium‑sensing receptor‑mediated apoptosis. Int J Mol Med 47: 302-314, 2021.
APA
Du, S., Zhang, Y., Zhao, Y., Dong, Y., Tang, J., Zhou, X., & Gao, W. (2021). Astragaloside IV attenuates cerebral ischemia‑reperfusion injury in rats through the inhibition of calcium‑sensing receptor‑mediated apoptosis. International Journal of Molecular Medicine, 47, 302-314. https://doi.org/10.3892/ijmm.2020.4777
MLA
Du, S., Zhang, Y., Zhao, Y., Dong, Y., Tang, J., Zhou, X., Gao, W."Astragaloside IV attenuates cerebral ischemia‑reperfusion injury in rats through the inhibition of calcium‑sensing receptor‑mediated apoptosis". International Journal of Molecular Medicine 47.1 (2021): 302-314.
Chicago
Du, S., Zhang, Y., Zhao, Y., Dong, Y., Tang, J., Zhou, X., Gao, W."Astragaloside IV attenuates cerebral ischemia‑reperfusion injury in rats through the inhibition of calcium‑sensing receptor‑mediated apoptosis". International Journal of Molecular Medicine 47, no. 1 (2021): 302-314. https://doi.org/10.3892/ijmm.2020.4777
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Spandidos Publications style
Du SJ, Zhang Y, Zhao YM, Dong YJ, Tang JL, Zhou XH and Gao WJ: Astragaloside IV attenuates cerebral ischemia‑reperfusion injury in rats through the inhibition of calcium‑sensing receptor‑mediated apoptosis. Int J Mol Med 47: 302-314, 2021.
APA
Du, S., Zhang, Y., Zhao, Y., Dong, Y., Tang, J., Zhou, X., & Gao, W. (2021). Astragaloside IV attenuates cerebral ischemia‑reperfusion injury in rats through the inhibition of calcium‑sensing receptor‑mediated apoptosis. International Journal of Molecular Medicine, 47, 302-314. https://doi.org/10.3892/ijmm.2020.4777
MLA
Du, S., Zhang, Y., Zhao, Y., Dong, Y., Tang, J., Zhou, X., Gao, W."Astragaloside IV attenuates cerebral ischemia‑reperfusion injury in rats through the inhibition of calcium‑sensing receptor‑mediated apoptosis". International Journal of Molecular Medicine 47.1 (2021): 302-314.
Chicago
Du, S., Zhang, Y., Zhao, Y., Dong, Y., Tang, J., Zhou, X., Gao, W."Astragaloside IV attenuates cerebral ischemia‑reperfusion injury in rats through the inhibition of calcium‑sensing receptor‑mediated apoptosis". International Journal of Molecular Medicine 47, no. 1 (2021): 302-314. https://doi.org/10.3892/ijmm.2020.4777
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