Open Access

Kaiso regulates osteoblast differentiation and mineralization via the Itga10/PI3K/AKT signaling pathway

  • Authors:
    • Wenwen Tong
    • Jia Li
    • Xinzhe Feng
    • Chen Wang
    • Yihong Xu
    • Chongru He
    • Weidong Xu
  • View Affiliations

  • Published online on: February 5, 2021     https://doi.org/10.3892/ijmm.2021.4874
  • Article Number: 41
  • Copyright: © Tong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Bone homeostasis is maintained by a dynamic balance between bone formation and bone resorption. The cellular activities of osteoblasts and osteoclasts are the primary factors that maintain this dynamic balance. The transcription factor Kaiso has been identified as a regulator of cell proliferation and differentiation in various cells. However, research into its role in bone homeostasis is currently lacking. In the present study, cell and animal experiments were conducted to investigate the role of Kaiso in bone homeostasis. The present study identified that Kaiso was downregulated during osteoblast differentiation in MC3T3‑E1 cells. Gain‑ and loss‑of‑function studies in MC3T3‑E1 cells demonstrated that Kaiso served a critical role in osteoblast differentiation in vitro. The findings were further confirmed in vivo. The results of the sequence analysis indicated that Kaiso influenced osteoblast differentiation and mineralization by regulating the PI3K/AKT signaling pathway. Moreover, integrin subunit α10 (Itga10) was identified as a direct target of Kaiso via chromatin immunoprecipitation and luciferase reporter assays. Collectively, these findings suggested that Kaiso regulated the differentiation of osteoblasts via the Itga10/PI3K/AKT pathway, which represents a therapeutic target for bone formation or bone resorption‑related diseases.
View Figures
View References

Related Articles

Journal Cover

April-2021
Volume 47 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Tong W, Li J, Feng X, Wang C, Xu Y, He C and Xu W: Kaiso regulates osteoblast differentiation and mineralization via the Itga10/PI3K/AKT signaling pathway. Int J Mol Med 47: 41, 2021.
APA
Tong, W., Li, J., Feng, X., Wang, C., Xu, Y., He, C., & Xu, W. (2021). Kaiso regulates osteoblast differentiation and mineralization via the Itga10/PI3K/AKT signaling pathway. International Journal of Molecular Medicine, 47, 41. https://doi.org/10.3892/ijmm.2021.4874
MLA
Tong, W., Li, J., Feng, X., Wang, C., Xu, Y., He, C., Xu, W."Kaiso regulates osteoblast differentiation and mineralization via the Itga10/PI3K/AKT signaling pathway". International Journal of Molecular Medicine 47.4 (2021): 41.
Chicago
Tong, W., Li, J., Feng, X., Wang, C., Xu, Y., He, C., Xu, W."Kaiso regulates osteoblast differentiation and mineralization via the Itga10/PI3K/AKT signaling pathway". International Journal of Molecular Medicine 47, no. 4 (2021): 41. https://doi.org/10.3892/ijmm.2021.4874