Kaiso regulates osteoblast differentiation and mineralization via the Itga10/PI3K/AKT signaling pathway

Tong,Wenwen; Li,Jia; Feng,Xinzhe; Wang,Chen; Xu,Yihong; He,Chongru; Xu,Weidong

doi:10.3892/ijmm.2021.4874

Kaiso regulates osteoblast differentiation and mineralization via the Itga10/PI3K/AKT signaling pathway

Authors:
- Wenwen Tong
- Jia Li
- Xinzhe Feng
- Chen Wang
- Yihong Xu
- Chongru He
- Weidong Xu
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Affiliations: Department of Joint Bone Disease Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China
Published online on: February 5, 2021 https://doi.org/10.3892/ijmm.2021.4874
Article Number: 41
Copyright: © Tong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Bone homeostasis is maintained by a dynamic balance between bone formation and bone resorption. The cellular activities of osteoblasts and osteoclasts are the primary factors that maintain this dynamic balance. The transcription factor Kaiso has been identified as a regulator of cell proliferation and differentiation in various cells. However, research into its role in bone homeostasis is currently lacking. In the present study, cell and animal experiments were conducted to investigate the role of Kaiso in bone homeostasis. The present study identified that Kaiso was downregulated during osteoblast differentiation in MC3T3‑E1 cells. Gain‑ and loss‑of‑function studies in MC3T3‑E1 cells demonstrated that Kaiso served a critical role in osteoblast differentiation in vitro. The findings were further confirmed in vivo. The results of the sequence analysis indicated that Kaiso influenced osteoblast differentiation and mineralization by regulating the PI3K/AKT signaling pathway. Moreover, integrin subunit α10 (Itga10) was identified as a direct target of Kaiso via chromatin immunoprecipitation and luciferase reporter assays. Collectively, these findings suggested that Kaiso regulated the differentiation of osteoblasts via the Itga10/PI3K/AKT pathway, which represents a therapeutic target for bone formation or bone resorption‑related diseases.

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