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Article

Knockdown of long non‑coding RNA HCP5 suppresses the malignant behavior of retinoblastoma by sponging miR‑3619‑5p to target HDAC9

  • Authors:
    • Yuguang Zhu
    • Fengqin Hao
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 261041, P.R. China
  • Article Number: 74
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    Published online on: March 3, 2021
       https://doi.org/10.3892/ijmm.2021.4907
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Abstract

A number of studies have verified the vital effects of long non‑coding RNAs on the malignant behaviour of retinoblastoma (RB). The objective of the present study was to examine the specific role and mechanisms of HLA complex P5 (HCP5) in RB. For this purpose, reverse transcription‑quantitative polymerase chain reaction was used to determine the expression of HCP5, microRNA (miRNA/miR)‑3619‑5p and histone deacetylase 9 (HDAC9). A 3‑(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2‑H-tetrazolium bromide assay was conducted to detect cell viability. Transwell assays were used to evaluate the abilities of cell migration and invasion. A mouse tumor model was established to explore the functions of HCP5 in RB in vivo. The interactions between HCP5, miR‑3619‑5p and HDAC9 were confirmed by a dual‑luciferase reporter assay. The protein expression of HDAC9 was determined by western blot analysis. The results revealed that the expression levels of HCP5 and HDAC9 were upregulated, whereas those of miR‑3619‑5p were downregulated in RB tissues and cell lines. The downregulation of HCP5 or the overexpression of miR‑3619‑5p suppressed RB cell proliferation, migration and invasion in vitro. Simultaneously, the knockdown of HCP5 suppressed tumor growth in mice in vivo. In addition, HCP5 was directly bound to miR‑3619‑5p and inversely correlated with miR‑3619‑5p. HDAC9 was found to be a target gene of and negatively regulated by miR‑3619‑5p. HCP5 expression also positively correlated with HDAC9 expression. Rescue experiments revealed that the overexpression of HDAC9 or the inhibition of miR‑3619‑5p reversed the inhibition of RB cell viability, migration and invasion induced by the knockdown of HCP5. On the whole, the present study demonstrates that the silencing of HCP5 exerts an anti‑tumor effect in RB by sponging miR‑3619‑5p to target HDAC9.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu Y and Hao F: Knockdown of long non‑coding RNA HCP5 suppresses the malignant behavior of retinoblastoma by sponging miR‑3619‑5p to target HDAC9. Int J Mol Med 47: 74, 2021.
APA
Zhu, Y., & Hao, F. (2021). Knockdown of long non‑coding RNA HCP5 suppresses the malignant behavior of retinoblastoma by sponging miR‑3619‑5p to target HDAC9. International Journal of Molecular Medicine, 47, 74. https://doi.org/10.3892/ijmm.2021.4907
MLA
Zhu, Y., Hao, F."Knockdown of long non‑coding RNA HCP5 suppresses the malignant behavior of retinoblastoma by sponging miR‑3619‑5p to target HDAC9". International Journal of Molecular Medicine 47.5 (2021): 74.
Chicago
Zhu, Y., Hao, F."Knockdown of long non‑coding RNA HCP5 suppresses the malignant behavior of retinoblastoma by sponging miR‑3619‑5p to target HDAC9". International Journal of Molecular Medicine 47, no. 5 (2021): 74. https://doi.org/10.3892/ijmm.2021.4907
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu Y and Hao F: Knockdown of long non‑coding RNA HCP5 suppresses the malignant behavior of retinoblastoma by sponging miR‑3619‑5p to target HDAC9. Int J Mol Med 47: 74, 2021.
APA
Zhu, Y., & Hao, F. (2021). Knockdown of long non‑coding RNA HCP5 suppresses the malignant behavior of retinoblastoma by sponging miR‑3619‑5p to target HDAC9. International Journal of Molecular Medicine, 47, 74. https://doi.org/10.3892/ijmm.2021.4907
MLA
Zhu, Y., Hao, F."Knockdown of long non‑coding RNA HCP5 suppresses the malignant behavior of retinoblastoma by sponging miR‑3619‑5p to target HDAC9". International Journal of Molecular Medicine 47.5 (2021): 74.
Chicago
Zhu, Y., Hao, F."Knockdown of long non‑coding RNA HCP5 suppresses the malignant behavior of retinoblastoma by sponging miR‑3619‑5p to target HDAC9". International Journal of Molecular Medicine 47, no. 5 (2021): 74. https://doi.org/10.3892/ijmm.2021.4907
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